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Influence of diisocyanate reactivity and water solubility on the formation and the mechanical properties of gelatin-based networks in water

Published online by Cambridge University Press:  28 June 2013

Tim Gebauer ,
Axel T. Neffe  and
Andreas Lendlein
Tim Gebauer
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513 Teltow, Germany Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm, Germany Helmholtz Virtual Institute – Multifunctional Biomaterials for Medicine, Teltow and Berlin, Germany
Axel T. Neffe
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513 Teltow, Germany Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm, Germany Helmholtz Virtual Institute – Multifunctional Biomaterials for Medicine, Teltow and Berlin, Germany
Andreas Lendlein
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513 Teltow, Germany Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm, Germany Helmholtz Virtual Institute – Multifunctional Biomaterials for Medicine, Teltow and Berlin, Germany
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Abstract

Gelatin can be covalently crosslinked in aqueous solution by application of diisocyanates like L-lysine diisocyanate ethyl ester in order to form hydrogels. Reaction of isocyanate groups with water is however a limiting factor in hydrogel network formation and can strongly influence the outcome of the crosslinking process. Here, diisocyanates with different water solubility and reactivity were applied for the formation of gelatin-based hydrogel networks and the mechanical properties of the hydrogels were investigated to gain a better understanding of starting material/ hydrogel property relations. L-Lysin diisocyanate ethyl ester (LDI), 2,4-toluene diisocyanate (TDI), 1,4-butane diisocyanate (BDI), and isophorone diisocyanate (IPDI) were selected, having different solubility in water ranging from 10-4 to 10-2 mol·L-1. BDI and LDI were estimated to have average reactive isocyanates groups, whereas TDI is highly reactive and IPDI has low reactivity. Formed hydrogels showed different morphologies and were partially very inhomogeneous. Gelation time (1 to 50 minutes), water uptake (300 to 900 wt.-%), and mechanical properties determined by tensile tests (E-moduli 35 to 370 kPa) and rheology (Shear moduli 4.5 to 19.5 kPa) showed that high water solubility as well as high reactivity leads to the formation of poorly crosslinked or inhomogeneous materials. Nevertheless, diisocyanates with lower solubility in water and low reactivity are able to form stable, homogeneous hydrogel networks with gelatin in water.

Keywords

biomaterialchemical reactionreactivity
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium NN – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 15 - 20
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Nielsen, L.E., J. Macromol. Sci. - Revs. Macromol. Chem., 1969, 3, 69 CrossRefGoogle Scholar
Van Vlierberghe, S., Dubruel, P., Schacht, E., Biomacromol., 2011, 12, 1387 CrossRefGoogle Scholar
Olde Damink, L.H.H., Dijkstra, P.J., van Luyn, M.J.A., van Wachem, P.B., Nieuwenhuis, P., Feijen, J., J. Mater. Sci.: Mat. Med., 1995, 6, 429 Google Scholar
Nowatzki, P.J., Tirrell, D.A., Biomaterials, 2004, 25, 1261 CrossRefGoogle Scholar
Digenis, G.A., Gold, T.B., Shah, V.P., J. Pharm. Sci., 1994, 83, 915 CrossRefGoogle Scholar
Tronci, G., Neffe, A.T., Pierce, B.F., Lendlein, A., J. Mater. Chem., 2010, 20, 8875 CrossRefGoogle Scholar
Pierce, B.F., Pittermann, E., Ma, N., Gebauer, T., Neffe, A.T., Hölscher, M., Jung, F., Lendlein, A., Macromol. Biosci., 2012, 12, 312 CrossRefGoogle Scholar
Arnold, R.G., Nelson, J.A., Verbanc, J.J., Chem. Revs., 1957, 57, 47 CrossRefGoogle Scholar
Ulrich, H., J. Polymer Sci.: Macromol. Rev., 1976, 11, 93 Google Scholar
Ionescu, M., Chemistry and Technology of Polyols for Polyurethanes, Repra Technology Ltd., Shawsbury, UK, 2005 Google Scholar
Neffe, A.T., Tronci, G., Alteheld, A., Lendlein, A., Macromol. Chem. Phys., 2010, 211, 182 CrossRefGoogle Scholar
Orakdogen, N., Okay, O., Polymer Bulletin, 2006, 57, 631 CrossRefGoogle Scholar
Abdurrahmanoglu, S., Okay, O., J. Macromol. Sci., Part A: Pure and Applied Chem., 2008, 45, 769 CrossRefGoogle Scholar