Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-04T21:12:45.074Z Has data issue: false hasContentIssue false
  • English
  • Français

Fibrinogen Adsorption on Hydroxyapatite, Carbonate Apatite and Gold Surfaces In Situ Detected by Quartz Crystal Microbalance with Resistance Technique

Published online by Cambridge University Press:  18 May 2012

Hiroshi Yonekura ,
Motohiro Tagaya ,
Tomohiko Yoshioka ,
Toshiyuki Ikoma  and
Junzo Tanaka
Hiroshi Yonekura
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Motohiro Tagaya
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Tomohiko Yoshioka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Toshiyuki Ikoma
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Junzo Tanaka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Get access

Abstract

When a biomaterial is implanted into the body, blood proteins adsorb on its surface and subsequently cells adhere via the protein adlayer. Thus, the understanding of protein adsorption and conformational change on the biomaterial surfaces is of great importance to control the biocompatibility such as antithrombotic properties and cell adhesion behaviors. In this study, we synthesized hydroxyapatite (HAp) and carbonate apatite (CAp) by a wet method. Then we successfully fabricated the HAp and CAp sensors for QCM-R by an electrophoretic deposition method. Adsorption behavior of proteins on the bone substitute material can be monitored by using these apatite sensors. Bovine serum albumin and fibrinogen were employed for the model proteins, and monitored the adsorption behavior on the HAp, CAp and reference gold (Au) sensors by the QCM-R technique. As a result, we revealed that fibrinogen and bovine serum albumin adsorbs on the gold surface by hydrophobic interaction, and adsorbs on the HAp and CAp surfaces mainly by electrostatic force. Besides, we revealed that fibrinogen adsorbs on the Au surface more rigid than on the HAp and CAp surfaces while bovine serum albumin adsorbs on the HAp and CAp surface more rigidly than on the Au surface.

Keywords

biomaterialsurface chemistryCa
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm10-25
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Dorozhkin, Sergey V., J Mater Sci (2007) 42, 1061–1095.CrossRefGoogle Scholar
2. Tonegawa, Toru, Ikoma, Toshiyuki, Yoshioka, Tomohiko, Hanagata, Nobutaka, Tanaka, Junzo, J Mater Sci (2010) 45: 2419–2426.CrossRefGoogle Scholar
3. Su, Xiao-Li, Li, Yanbin, “A QCM immunosensor for Salmonella detection with simultaneous measurements of resonant frequency and motional resistance,” Biosensors and Bioelectronics 21 (2005) 840–848.CrossRefGoogle ScholarPubMed
4. Marx, Kenneth A, Zhou, Tiean, Montrone, Anne, Mclntosh, Donna, Braunhut, Susan J., “Quartz crystal microbalance biosensor study of endothelial cells and their extracellular matrix following cell removal: Evidence for transient cellular stress and viscoelastic changes during detachment and the elastic behavior of the pure matrix,” Analytical Biochemistry 343 (2005) 23–24.CrossRefGoogle ScholarPubMed
5. Monkawa, Akira, Ikoma, Toshiyuki, Yunoki, Shunji, Yoshioka, Tomohiko, Tanaka, Junzo, Chakarov, Dinko, Kasemo, Bengt, “Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique,” Biomaterials 27 (2006) 5748–5754.10.1016/j.biomaterials.2006.07.029CrossRefGoogle Scholar