Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-29T07:51:47.972Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of a Porous Silicon Product for Small Molecule Mass Spectrometry

Published online by Cambridge University Press:  21 March 2011

Grace Credo ,
Hillary Hewitson ,
Christopher Benevides  and
Edouard S. P. Bouvier
Grace Credo
Affiliation:
Advanced Separations Technology Group (R&D Engineering)
Hillary Hewitson
Affiliation:
Life Sciences R&D, Waters Corporation, Milford, Massachusetts, 01757, U.S.A.
Christopher Benevides
Affiliation:
Life Sciences R&D, Waters Corporation, Milford, Massachusetts, 01757, U.S.A.
Edouard S. P. Bouvier
Affiliation:
Advanced Separations Technology Group (R&D Engineering)
Get access

Abstract

Previous work has demonstrated the utility of desorption/ionization on silicon (DIOS) time-of-flight mass spectrometry (TOFMS) in drug molecule and peptide detection[1-7]. In this work, the utility of DIOS for small molecule detection is established using commercially available porous silicon (por Si)-based target plates for MS. Since the morphology and handling of the substrates can have dramatic effects on the MS characteristics, the development of consistent manufacturing methods and characterization protocols has been central to the production of reproducible target plates[7]. Using sample substrates manufactured in-house, we show that 1) small molecules and protein digests were detected without matrix-related peaks, 2) por Si morphology was optimized for small molecule detection, 3) reproducible DIOS plates were produced, 4) although the target plates were shown to be sensitive to contamination, a consistent cleaning procedure was developed to remove contaminants, and 5) stability and shelf life were characterized as a function of surface derivatization. Dynamic range, sensitivity, quantitation, speed of analysis, solution composition, and automated deposition have also been evaluated and are described in related work[7-9]. Potential applications include high-throughput small molecule assays for drug discovery[10a] and high sensitivity (sub-femtomole) peptide identification for proteomics[10b].

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 808: Symposium A – Amorphous and Nanocrystaline Silicon Science and Technology-2004 , 2004 , A4.28
Copyright
Copyright © Materials Research Society 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Wei, J., Buriak, J. M., Siuzdak, G., Nature 399, 243–246 (1999).Google Scholar
2. Shen, Z., Thomas, J. J., Averbuj, C., Broo, K. M., Engelhard, M., Crowell, J. E., Finn, M. G., Siuzdak, G., Anal. Chem. 73, 612–619 (2001).Google Scholar
3. Thomas, J. J., Shen, Z., Crowell, J. E., Finn, M. G., Siuzdak, G., Proc. Natl. Acad. Sci. 98, 4932–4937 (2001).Google Scholar
4. Kruse, R.A., Li, X., Bohn, P.W., and Sweedler, J.V., Anal. Chem. 73, 3639–3645 (2001).Google Scholar
5. Tuomikoski, S., Huikko, K., Grigoras, K., Ostman, P., Kostiainen, R., Baumann, M., Abian, J., Kotiaho, T., Franssila, S., Lab Chip 2, 247–253 (2002).Google Scholar
6. Huikko, K., Ostman, P., Sauber, C., Mandel, F., Grigoras, K., Franssila, S., Kotiaho, T., and Kostiainen, R., Rapid Comm. Mass Spec. 17, 1339–1343 (2003).Google Scholar
7.(a) Credo, G. M., Hewitson, H. B., Fountain, K. J., Gilar, M., Finch, J. W., Stumpf, C. L., Benevides, C. C., Bouvier, E. S. P., Compton, B. J., Shen, Z., Siuzdak, G., poster, 51st ASMS Conf., 2003 (Montreal, Canada). (b) G. M. Credo, H.B. Hewitson, D.B. Wall, J.W. Finch, C.C. Benevides, E.S.P. Bouvier, B.J. Compton, Z. Shen, G. Siuzdak, poster, IMSC Conf., 2003 (Edinburgh, UK).Google Scholar
8. Wall, D. B., Finch, J. W., Cohen, S. C., submitted.Google Scholar
9. Wall, D. B., Finch, J. W., Cohen, S. C., in progress.Google Scholar
10.(a) Shen, Z., Go, E. P., Gamez, A., Apon, J. V., Fokin, V., Greig, M., Ventura, M., Crowell, J. E., Blixt, O., Paulson, J. C., Stevens, R., Finn, M. G., Siuzdak, G., in press. (b) S. Trauger, E. P. Go, J. V. Apon, M. G. Finn, B. Compton, Z. Shen, G. Siuzdak, submitted. 11. A review of small molecule MALDI analysis: L. C. Cohen, Gusev, A. I., Anal. Bioanal. Chem. 373, 571-586 (2002).Google Scholar
12. Lehmann, V., Electrochemistry of Silicon, Wiley-VCH, Weinheim (Germany), 2002.Google Scholar