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Advanced Characterization Techniques for Nanoparticles for Cancer Research: Applications of SEM and NanoSIMS for Locating Au Nanoparticles in Cells

Published online by Cambridge University Press:  13 May 2013

Paul J Kempen ,
Chuck Hitzman ,
Laura S Sasportas ,
Sanjiv S Gambhir  and
Robert Sinclair
Paul J Kempen
Affiliation:
Department of Material Science and Engineering, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4034 U.S.A.
Chuck Hitzman
Affiliation:
Stanford Nanocharacterization Laboratory, Stanford University, 476 Lomita Mall, Stanford, CA 94305-4035 U.S.A.
Laura S Sasportas
Affiliation:
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 318 Campus Drive, Stanford, CA 94305-5427 U.S.A.
Sanjiv S Gambhir
Affiliation:
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 318 Campus Drive, Stanford, CA 94305-5427 U.S.A.
Robert Sinclair
Affiliation:
Department of Material Science and Engineering, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4034 U.S.A.
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Abstract

The ability of nano secondary ion mass spectrometry (NanoSIMS) to locate and analyze Raman active gold core nanoparticles (R-AuNPs) in a biological system is compared with the standard analysis using the scanning electron microscope (SEM). The same cell with R-AuNPs on and inside the macrophage was analyzed with both techniques to directly compare them. SEM analysis showed a large number of nanoparticles within the cell. Subsequent NanoSIMS analysis showed fewer R-AuNPs with lower spatial resolution. SEM was determined to be superior to NanoSIMS for the analysis of inorganic nanoparticles in complex biological systems.

Keywords

scanning electron microscopy (SEM)secondary ion mass spectroscopy (SIMS)biological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium MM – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 157 - 163
Copyright
Copyright © Materials Research Society 2013 

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References

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