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Efficient Production of Fluorescent Polydiacetylene-Containing Liposomes for Pathogen Detection and Identification

Published online by Cambridge University Press:  09 October 2013

Cassandra J. Wright-Walker ,
Caroline E. Hansen ,
Michael A. Evans ,
Emily S. Nyers  and
Timothy W. Hanks
Cassandra J. Wright-Walker
Affiliation:
Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, U.S.A.
Caroline E. Hansen
Affiliation:
Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, U.S.A.
Michael A. Evans
Affiliation:
Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, U.S.A.
Emily S. Nyers
Affiliation:
Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, U.S.A.
Timothy W. Hanks
Affiliation:
Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, U.S.A.
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Abstract

Amphiphilic diacetylenes (DAs) can self-assemble into photopolymerizable liposomes that can be used to construct effective pathogen sensors. Here, modified commercial inkjet printers are used to disperse DAs into water, facilitating self-assembly. The liposomes are of similar size, but are significantly less polydisperse than liposomes formed using conventional sonication methods. The process is efficient, readily scalable and tolerant of structural modification. The derivitization of approximately 5% of the DA head groups and the incorporation of fluorophores into the hydrophobic bilayer allows for the preparation of novel multifluorophore PDA sensing systems that can provide enhanced bacterial discrimination in a single experiment by way of a fluorescent fingerprint.

Keywords

sensornanostructureink-jet printing
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium QQ – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 201 - 206
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Reppy, M. A. and Pindzola, B. A., “Biosensing with polydiacetylene materials: structures, optical properties and applications,” Chem. Commun., 4317–4338 (2007).CrossRefGoogle ScholarPubMed
Lasic, D. D., “Chapter 10 Applications of liposomes,” in Handbook of Biological Physics, Anonymous (North-Holland), pp. 491–519 (1995).CrossRefGoogle Scholar
Taylor, T. M., Weiss, J., Davidson, P. M., and Bruce, B. D., “Liposomal Nanocapsules in Food Science and Agriculture,” Crit. Rev. Food Sci. Nutr. 45, 587–605 (2005).CrossRefGoogle ScholarPubMed
Hauschild, S., Lipprandt, U., Rumplecker, A., Borchert, U., Rank, A., Schubert, R., and Förster, S., “Direct Preparation and Loading of Lipid and Polymer Vesicles Using Inkjets,” Small 1, 1177–1180 (2005).CrossRefGoogle ScholarPubMed
Owczarczak, A. B., Shuford, S. O., Wood, S. T., Deitch, S., and Dean, D., “Creating transient cell membrane pores using a standard inkjet printer.” J Vis Exp. e3681, doi:10.3791/3681 (2012).Google ScholarPubMed
Rangin, M. and Basu, A., “Lipopolysaccharide Identification with Functionalized Polydiacetylene Liposome Sensors.” J. Am. Chem. Soc. 126, 5038–5039 (2004).CrossRefGoogle ScholarPubMed