Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-23T11:11:43.560Z Has data issue: false hasContentIssue false

Efficient Analysis of Single Molecule Spectroscopic Data via MATLAB

Published online by Cambridge University Press:  21 December 2012

Caleb M. Hill
Affiliation:
The University of Alabama, Box 870336, Tuscaloosa, AL35487
Shanlin Pan
Affiliation:
The University of Alabama, Box 870336, Tuscaloosa, AL35487
Get access

Abstract

A MATLAB program is developed to help analyze single molecule spectroscopic data obtained with a standard inverted optical microscope. The described software has provisions to mitigate the effects of high background signals present in such data sets and greatly streamlines their analysis. Efficient single molecule image analysis and statistical blinking analysis are enabled with these programs to support single molecule imaging in an inverted configuration.

Type
Articles
Copyright
Copyright © Materials Research Society 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Huser, Thomas, Yan, Ming and Rothberg, Lewis J., Proceedings of the National Academy of Sciences 97, 11187 (2000).CrossRefGoogle Scholar
Schindler, Florian, Lupton, John M., Muller, Josef, Feldmann, Jochen and Scherf, Ullrich, Nat. Mater. 5, 141 (2006).CrossRefGoogle Scholar
Feist, Florian A., Tommaseo, Giovanni and Basché, Thomas, J. Phys. Chem. C 113, 11484 (2009).CrossRefGoogle Scholar
Park, So-Jung, Gesquiere, Andre J., Yu, Ji and Barbara, Paul F., J. Am. Chem. Soc. 126, 4116 (2004).CrossRefGoogle Scholar
Palacios, E., Fan, Fu-Ren F., Bard, Allen J. and Barbara, Paul F., J. Am. Chem. Soc. 128, 9028 (2006).CrossRefGoogle Scholar
Palacios, Rodrigo E., Fan, Fu-Ren F., Grey, John K., Suk, Jungdon, Bard, Allen J. and Barbara, Paul F., Nat. Mater. 6, 680 (2007).CrossRefGoogle Scholar
Palacios, Rodrigo E., Chang, Wei-Shun, Grey, John K., Chang, Ya-Lan, Miller, William L., Lu, Chun-Yaung, Henkelman, Graeme, Zepeda, Danny, Ferraris, John and Barbara, Paul F., J. Phys. Chem. B 113, 14619 (2009).CrossRefGoogle Scholar
Biju, Vasudevanpillai, Micic, Miodrag, Hu, Dehong and Lu, H. P., J. Am. Chem. Soc. 126, 9374 (2004).CrossRefGoogle Scholar
Wang, Yuanmin, Wang, Xuefei, Kumar Ghosh, Sujit and Lu, H. P., J. Am. Chem. Soc. 131, 1479 (2009).CrossRefGoogle Scholar
Tachikawa, Takashi, Cui, Shi-Cong, Fujitsuka, Mamoru and Majima, Tetsuro, Phys. Chem. Chem. Phys. 14, 4244 (2012).CrossRefGoogle Scholar
Vogelsang, Jan and Lupton, John M., J. Phys. Chem. Lett. 3, 1503 (2012).CrossRefGoogle Scholar
Wollman, Roy and Stuurman, Nico, Journal of Cell Science 120, 3715 (2007).CrossRefGoogle Scholar