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Comparing Biocompatibility of Nanocrystalline Titanium and Titanium-Oxide with Microcrystalline Titanium

Published online by Cambridge University Press:  15 July 2013

Raheleh Miralami ,
Laura Koepsell ,
Thyagaseely Premaraj ,
Bongok Kim ,
Geoffrey M. Thiele ,
J. Graham Sharp ,
Kevin L. Garvin  and
Fereydoon Namavar
Raheleh Miralami
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE
Laura Koepsell
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE
Thyagaseely Premaraj
Affiliation:
Department of Growth & Development -, University of Nebraska-Lincoln, Lincoln, NE
Bongok Kim
Affiliation:
Department of Adult Restorative Dentistry, University of Nebraska - Lincoln, Lincoln, NE
Geoffrey M. Thiele
Affiliation:
Department of Internal Medicine – Rheumatology, UNMC, Omaha, NE
J. Graham Sharp
Affiliation:
Department of Genetics, Cell Biology & Anatomy, UNMC, Omaha, NE
Kevin L. Garvin
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE
Fereydoon Namavar*
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE
*
*Corresponding Author [email protected]
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Abstract

Titanium (Ti) is the material of choice for orthopaedic applications because it is biocompatible and encourages osteoblast ingrowth. It was shown that the biocompatibility of Ti metal is due to the presence of a thin native sub-stoichiometric titanium oxide layer which enhances the adsorption of mediating proteins on the surface [1]. The present studies were devised to evaluate the adhesion, survival, and growth of cells on the surface of new engineered nano-crystal films of titanium and titanium oxides and compare them with orthopaedic-grade titanium with microcrystals. The engineered nano-crystal films with hydrophilic properties are produced by employing an ion beam assisted deposition (IBAD) technique. IBAD combines physical vapor deposition with concurrent ion beam bombardment in a high vacuum environment to produce films (with 3 to 70 nm grain size) with superior properties. These films are “stitched” to the artificial orthopaedic implant materials with characteristics that affect the wettability and mechanical properties of the coatings.

To characterize the biocompatibility of these nano-engineered surfaces, we have studied osteoblast function including cell adhesion, growth, and differentiation on different nanostructured samples. Cell responses to surfaces were examined using SAOS-2 osteoblast-like cells. We also studied a correlation between the surface nanostructures and the cell growth by characterizing the SAOS-2 cells with immunofluorescence and measuring the amount alizarin red concentration produced after 7 and 14 days. The number of adherent cells was determined by means of nuclei quantification on the nanocrystalline Ti, TiO2, and microcrystalline Ti and analysis was performed with Image J. Our experimental results indicated that nanocrystalline TiO2 is superior to both nano and microcrystalline Ti in supporting growth, adhesion, and proliferation. Improving the quality of surface oxide, i.e. fabricating stoichiometric oxides as well as nanoengineering the surface topology, is crucial for increasing the biocompatibility of Ti implant materials.

Keywords

ion-beam assisted depositionbiomedicalcrystalline
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium NN – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 91 - 96
Copyright
Copyright © Materials Research Society 2013 

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References

Namavar, F., Sabirianov, R., et al. , presented at the 2012 Annual Meeting of the Orthopaedic Research Society, San Francisco, Feb 2012 (unpublished).Google Scholar
Lord, M., Foss, M., Besenbacher, F., Nano Today 5, 66–78 (2010).CrossRefGoogle Scholar
Webster, T.J., Ergun, C., et al. , J. Biomed. Mater. Res. A 67(3), 975–80 (2003).CrossRefGoogle Scholar
Svehla, M., Morberg, P., Bruce, W., Zicat, B., et al. , J. Arthroplasty 17(3), 304–11 (2002).CrossRefGoogle Scholar
Klokkevold, P.R., Johnson, P., Dadgostari, S., et al. , Clin. Oral. Implants. Res. 12, (4), 350 (2001).CrossRefGoogle Scholar
Brunette, D.M., Chehroudi, B., J. Biomech. Eng. 121(1), 49–57(1999).CrossRefGoogle Scholar
Namavar, F., Cheung, C.L., Sabirianov, R.F., et al. , NanoLetters 8 (4) 988 (2008).CrossRefGoogle Scholar
Kalbacova, M., Rezek, B., Baresova, V., et al. , Acta Biomater. 5(8) 3076–85 (2009).CrossRefGoogle Scholar
Kim, J.B., Leucht, P., Luppen, C.A., et al. , Bone 41(1) 39–51 (2007).CrossRefGoogle ScholarPubMed
Jager, M., et al. , J. Biomed. Biotechnol. 8, 69036 (2007).Google Scholar
Stevens, M.M., George, J.H., Science 310 (5751) 1135–8 (2005).CrossRefGoogle Scholar
Le Guehennec, L., Lopez-Heredia, M.A., Enkel, B., et al. , Acta Biomater. 4(3) 535–43 (2008).CrossRefGoogle Scholar
Francois, P., Vaudaux, P., Taborelli, M., et al. , Clin. Oral Implants Res. 8(3) 217–25 (1997).CrossRefGoogle Scholar
Wu, Y., Zitelli, J.P., TenHuisen, K.S., Yu, X., Libera, M.R., Biomaterials. 32(4) 951–60 (2011).CrossRefGoogle Scholar
Boyan, B.D., Boneweld, L.F., Paschalis, E.P., et al. , Calcif. Tissue Int. 71(6) 519–29 (2002).CrossRefGoogle Scholar
Babchanko, O., Kromka, A., et al. , Physica. Status Solidi A 206(9) 2033–7 (2009).CrossRefGoogle Scholar
Sasmazel, H.T., Int. J. Biol. Macromol. 49, 838–846 (2011).CrossRefGoogle Scholar
Mendonca, G., Mendonca, D.B., Aragao, F.J., et al. , Biomaterials 29(28) 3822–3835 (2008).CrossRefGoogle Scholar
Namavar, F., Sabirianov, R.F., et al. in Engineered Nanostructured Coatings for Enhanced Protein Adsorption and Cell Growth (Mater. Res. Soc. Symp. Proc. 1418, Boston, MA 2011).Google Scholar
Sabirianov, R.F., Rubinstein, A., Namavar, F., Phys. Chem. Chem. Phys. 13 (14) 6597 (2011).CrossRefGoogle Scholar