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Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels

Published online by Cambridge University Press:  11 August 2017

Ioannis Azoidis ,
Joel Metcalfe ,
James Reynolds ,
Shirley Keeton ,
Sema S. Hakki ,
Jonathan Sheard  and
Darius Widera Open the ORCID record for Darius Widera [Opens in a new window]
Ioannis Azoidis
Affiliation:
Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Whiteknights Campus, Reading RG6 6AP, UK
Joel Metcalfe
Affiliation:
Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Whiteknights Campus, Reading RG6 6AP, UK
James Reynolds
Affiliation:
Lein Applied Diagnostics Ltd, Reading Enterprise Centre, Whiteknights Road, Reading RG6 6BU, UK
Shirley Keeton
Affiliation:
Cell Migration Lab, School of Biological Sciences, University of Reading, Whiteknights Campus, Reading RG6 6AP, UK
Sema S. Hakki
Affiliation:
Selcuk University Faculty of Dentistry Department of Periodontology Campus, 42079 Konya, Turkey
Jonathan Sheard
Affiliation:
Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Whiteknights Campus, Reading RG6 6AP, UK Sheard BioTech Limited, Suite LP36133, 20-22, Wenlock Road, London N17GU, UK
Darius Widera*
Affiliation:
Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Whiteknights Campus, Reading RG6 6AP, UK
*
Address all correspondence to Darius Widera at [email protected]
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Abstract

Human mesenchymal stem cells (MSCs) are the most intensely studied and clinically used adult stem cell type. Conventional long-term cultivation of MSCs as a monolayer is known to result in a reduction of their functionality and viability. In addition, large volumes of cell culture medium are required to obtain cell quantities needed for their clinical use. In this proof of concept study, we cultivated human MSCs within a three-dimensional nanofibrillar cellulose (NFC) hydrogel. We show that NFC is biocompatible with human MSCs, and represents a feasible approach to upscaling of their culture.

Type
Biomaterials for 3D Cell Biology Research Letters
Information
MRS Communications , Volume 7 , Issue 3 , September 2017 , pp. 458 - 465
Copyright
Copyright © Materials Research Society 2017 

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