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Selective immobilization of bacterial light-harvesting proteins and their photoelectric responses

Published online by Cambridge University Press:  17 August 2018

Rei Furukawa ,
Masaharu Kondo ,
Shunsuke Yajima ,
Kaori Harada ,
Kenji V.P. Nagashima ,
Morio Nagata ,
Kouji Iida ,
Takehisa Dewa  and
Mamoru Nango
Rei Furukawa*
Affiliation:
The University of Electro-Communications, Chofugaoka 1-5-1, Chofu, Tokyo 182-8585, Japan
Masaharu Kondo
Affiliation:
Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi 466-8555, Japan
Shunsuke Yajima
Affiliation:
Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi 466-8555, Japan
Kaori Harada
Affiliation:
Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi 466-8555, Japan
Kenji V.P. Nagashima
Affiliation:
Kanagawa University, Tsuchiya 2946, Hiratsuka, Kanagawa 259-1293, Japan
Morio Nagata
Affiliation:
Tokyo University of Science, 12-1 Ichigayafunagawara-cho, Shinjuku-ku, Tokyo 162-0826, Japan
Kouji Iida
Affiliation:
Nagoya Municipal Industrial Research Institute, Atsuta-ku, Nagoya 456-0058, Japan
Takehisa Dewa
Affiliation:
Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi 466-8555, Japan
Mamoru Nango
Affiliation:
Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi 466-8555, Japan OCARINA, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan
*
Address all correspondence to Rei Furukawa at [email protected]
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Abstract

With the aim of understanding the excitation energy transfer mechanism in natural photosynthetic membranes, light-harvesting (LH)2 and LH1-reaction center, which are pigment-protein complexes separated from Rhodobacter sphaeroides, were aligned on a planar electrode surface in stripe patterns at 5 µm intervals. Observation of the absorption spectrum and fluorescence microphotographs revealed selective immobilization and conservation of the pigments. Photocurrent signals were obtained when the electrode was illuminated at either 880 or 800 nm. The fabricated structure was confirmed to function as a natural photosynthetic membrane with the highest photocurrent signal being obtained when using a co-immobilized substrate under excitation at 800 nm.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 3 , September 2018 , pp. 1124 - 1128
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Copyright
Copyright © Materials Research Society 2018 

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