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PEDOT:PSS microelectrode arrays for hippocampal cell culture electrophysiological recordings

Published online by Cambridge University Press:  30 May 2017

Dimitrios A. Koutsouras ,
Adel Hama ,
Jolien Pas ,
Paschalis Gkoupidenis ,
Bruno Hivert ,
Catherine Faivre-Sarrailh ,
Eric Di Pasquale ,
Róisín M. Owens  and
George G. Malliaras
Dimitrios A. Koutsouras
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
Adel Hama
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
Jolien Pas
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
Paschalis Gkoupidenis
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
Bruno Hivert
Affiliation:
Aix Marseille University, CNRS, CRN2M, Marseille, France CSO@MyEnterix, 13344 Marseille, France
Catherine Faivre-Sarrailh
Affiliation:
Aix Marseille University, CNRS, CRN2M, Marseille, France CSO@MyEnterix, 13344 Marseille, France
Eric Di Pasquale
Affiliation:
Aix Marseille University, CNRS, CRN2M, Marseille, France CSO@MyEnterix, 13344 Marseille, France
Róisín M. Owens
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
George G. Malliaras*
Affiliation:
Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541 Gardanne, France
*
Address all Correspondence to George G. Malliaras at [email protected]
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Abstract

In vitro electrophysiology using microelectrode arrays (MEAs) plays an important role in understanding fundamental biologic processes, screening potential drugs and assessing the toxicity of chemicals. Low electrode impedance and ability to sustain viable cultures are the key technology requirements. We show that MEAs consisting of poly(3,4-ethylenedioxythiophene) doped with poly(styrene sulfonate) (PEDOT:PSS) and coated with poly-L-lysine satisfy these requirements. Hippocampal cell cultures, maintained for 3–6 weeks on these MEAs, give high quality recordings of neural activity. This enables the observation of drug-induced activity changes, which paves the way for using these devices in in vitro drug screening and toxicology applications.

Type
Research Letters
Information
MRS Communications , Volume 7 , Issue 2 , June 2017 , pp. 259 - 265
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

*

This author was an editor of this journal during the review and decision stage. For the MRC policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs.org/editor-manuscripts/.

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