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Decorin-containing collagen hydrogels as dimensionally stable scaffolds to study the effects of compressive mechanical loading on angiogenesis

Published online by Cambridge University Press:  20 July 2017

Marissa A. Ruehle ,
Laxminarayanan Krishnan ,
Steven A. LaBelle ,
Nick J. Willett ,
Jeffrey A. Weiss  and
Robert E. Guldberg
Marissa A. Ruehle
Affiliation:
Georgia Institute of Technology, Petit Institute for Bioengineering and Biosciences, Atlanta, GA, USA Emory University, Atlanta, GA, USA
Laxminarayanan Krishnan
Affiliation:
Georgia Institute of Technology, Petit Institute for Bioengineering and Biosciences, Atlanta, GA, USA
Steven A. LaBelle
Affiliation:
University of Utah, Salt Lake City, UT, USA
Nick J. Willett
Affiliation:
Georgia Institute of Technology, Petit Institute for Bioengineering and Biosciences, Atlanta, GA, USA Emory University, Atlanta, GA, USA Atlanta Veteran's Affairs Medical Center, Decatur, GA, USA
Jeffrey A. Weiss
Affiliation:
University of Utah, Salt Lake City, UT, USA
Robert E. Guldberg*
Affiliation:
Georgia Institute of Technology, Petit Institute for Bioengineering and Biosciences, Atlanta, GA, USA
*
Address all correspondence to R. E. Guldberg at [email protected]
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Abstract

Angiogenesis is a critical component during wound healing, and the process is sensitive to mechanical stimuli. Current in vitro culture environments used to investigate three-dimensional microvascular growth often lack dimensional stability and the ability to withstand compression. We investigated the ability of decorin (DCN), a proteoglycan known to modulate collagen fibrillogenesis, incorporated into a collagen hydrogel to increase construct dimensional stability while maintaining vascular growth. DCN did not affect microvascular growth parameters, while increasing the compressive modulus of collagen gels and significantly reducing the contraction of 3% collagen gels after 16 days in culture.

Type
Biomaterials for 3D Cell Biology Research Letters
Information
MRS Communications , Volume 7 , Issue 3 , September 2017 , pp. 466 - 471
Copyright
Copyright © Materials Research Society 2017 

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