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Antibody-conjugated near-infrared luminescent silicon quantum dots for biosensing

Published online by Cambridge University Press:  26 July 2019

Hiroto Yanagawa ,
Asuka Inoue ,
Hiroshi Sugimoto Open the ORCID record for Hiroshi Sugimoto [Opens in a new window] ,
Masahiko Shioi Open the ORCID record for Masahiko Shioi [Opens in a new window]  and
Minoru Fujii Open the ORCID record for Minoru Fujii [Opens in a new window]
Hiroto Yanagawa
Affiliation:
Technology Innovation Division, Panasonic Corporation, Yagumo-naka-machi, Moriguchi, Osaka 570-8501, Japan Department of Electrical and Electronic Engineering, Graduate School of Engineering, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
Asuka Inoue
Affiliation:
Department of Electrical and Electronic Engineering, Graduate School of Engineering, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
Hiroshi Sugimoto
Affiliation:
Department of Electrical and Electronic Engineering, Graduate School of Engineering, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
Masahiko Shioi
Affiliation:
Life Solutions Company, Panasonic Corporation, Kadoma, Osaka 571-8686, Japan
Minoru Fujii*
Affiliation:
Department of Electrical and Electronic Engineering, Graduate School of Engineering, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
*
Address all correspondence to Minoru Fujii at [email protected]
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Abstract

A process for bioconjugation of an IgG antibody and silicon quantum dots (Si-QDs) having the luminescence in the near-infrared (NIR) range was developed. For the bioconjugation, the surface of water-soluble all-inorganic Si-QDs was functionalized by using silane-coupling agents. In amino-functionalized Si-QDs, successful conjugation was achieved without strongly affecting the luminescence property. Detailed analyses revealed that Si-QDs are bound covalently to both the light and heavy chains of an IgG antibody. It was also confirmed that the binding property of an IgG antibody with antigen nucleoprotein was not ruined by the process. The successful conjugation of an IgG antibody and NIR luminescent Si-QDs paves the way for designing environmentally friendly bio-sensing and -imaging processes.

Type
Research Letters
Information
MRS Communications , Volume 9 , Issue 3 , September 2019 , pp. 1079 - 1086
Copyright
Copyright © The Author(s) 2019 

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