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Mitochondrion-selective hemicyanine dyes suitable for fiber laser excitation two-photon microscopy

Published online by Cambridge University Press:  03 September 2018

H. Moritomo ,
S. Onishi ,
N. Asamura ,
K. Matsumoto ,
Y. Suzuki  and
J. Kawamata
H. Moritomo
Affiliation:
Department of Integrated Science and Technology, National Institute of Technology Tsuyama College, 624-1 Numa, Tsuyama, Okayama 708-8509, Japan
S. Onishi
Affiliation:
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan
N. Asamura
Affiliation:
Department of Biology and Chemistry, Faculty of Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan
K. Matsumoto
Affiliation:
Department of Biology and Chemistry, Faculty of Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan
Y. Suzuki
Affiliation:
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan Department of Biology and Chemistry, Faculty of Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan
J. Kawamata*
Affiliation:
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan Department of Biology and Chemistry, Faculty of Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8512, Japan
*
Address all correspondence to J. Kawamata at [email protected]
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Abstract

We investigate the two-photon absorption characteristics of hemicyanine dyes that exhibit a one-photon absorption at around 500 nm. The dyes exhibited two-photon-induced fluorescence upon irradiation with an Yb-doped femtosecond fiber laser operating at 1030 nm. Among the dyes, 4-[4-[4-(dimethylamino)phenyl]-1,3-butadienyl]-1-ethyl-pyridinium perchlorate exhibited the most efficient two-photon-induced fluorescence at 1030 nm. Since these dyes possess cationic moiety, the dyes accumulated in the mitochondria of a living cell. Two-photon images of mitochondria were obtained by staining living HEK293 cells with these dyes. When 4-[4-[4-(dimethylamino)phenyl]-1,3-butadienyl]-1-ethyl-pyridinium perchlorate was employed, a two-photon-induced fluorescence image could be obtained even when a 3 mW fiber laser beam was used as the excitation source.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 3 , September 2018 , pp. 1064 - 1069
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Copyright
Copyright © Materials Research Society 2018 

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