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Grafting of glycerol methacrylate onto silicone rubber using γ-rays: derivatization to 2-oxoethyl methacrylate and immobilization of lysozyme

Published online by Cambridge University Press:  13 February 2018

G.G. Flores-Rojas ,
F. López-Saucedo ,
M. Quezada-Miriel  and
E. Bucio
G.G. Flores-Rojas*
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
F. López-Saucedo
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
M. Quezada-Miriel
Affiliation:
Instituo de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
E. Bucio*
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
*
Address all correspondence to G.G. Flores-Rojas and E. Bucio at [email protected], [email protected]
Address all correspondence to G.G. Flores-Rojas and E. Bucio at [email protected], [email protected]
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Abstract

The goal of this work was the modification of silicone rubber (SR) by radiation grafting of glycerol methacrylate (GlyMA) which was limited just on the surface, allowing the control of hydrophilicity and swelling properties. The grafted SRs were activated by derivatization of GlyMA to 2-oxoethyl methacrylate using sodium periodate, enabling the chemical immobilization of lysozyme by covalent bonds. The presence of lysozyme was confirmed by non-specific assay and by the enzymatic activity at 30 °C with Micrococcus lysodeikticus (coccus, Gram-positive). The materials were characterized by Fourier transform infrared spectroscopy-attenuated total reflectance, thermogravimetric analysis, water contact angle, and by mechanical properties as well as scanning electron microscope.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 1 , March 2018 , pp. 199 - 206
Copyright
Copyright © Materials Research Society 2018 

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