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Fluorescence loss of commercial aqueous quantum dots during preparation for bioimaging

Published online by Cambridge University Press:  29 April 2019

Kil Ho Lee ,
Thomas Porter  and
Jessica O. Winter
Kil Ho Lee
Affiliation:
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 W. Woodruff Ave. Columbus, OH 43210, USA
Thomas Porter
Affiliation:
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 W. Woodruff Ave. Columbus, OH 43210, USA
Jessica O. Winter*
Affiliation:
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 W. Woodruff Ave. Columbus, OH 43210, USA Department of Biomedical Engineering, The Ohio State University, 151 W. Woodruff Ave. Columbus, OH 43210, USA
*
Address all correspondence to Jessica O. Winter at [email protected]
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Abstract

Quantum dots (QDs) are increasingly employed in biologic imaging applications; however, anecdotal reports suggest difficulties in QD bioconjugation. Further, the stability of commercial QDs during bioconjugation has not been systematically evaluated. Thus, we examined fluorescence losses resulting from aggregation and declining photoluminescence quantum yield (QY) for commercial CdSe/ZnS QD products from four different vendors. QDs were most stable in the aqueous media in which they were supplied. The largest QY declines were observed during centrifugal filtration, whereas the largest declines in colloidal stability occurred in 2-(N-morpholino)ethanesulfonic acid (MES) buffer. These results enable optimization of bioconjugation protocols.

Type
Research Letters
Information
MRS Communications , Volume 9 , Issue 2 , June 2019 , pp. 702 - 709
Copyright
Copyright © Materials Research Society 2019 

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Footnotes

*

These authors have contributed equally to this work.

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