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Effect of the spacer arm on non-specific binding in membrane affinity chromatography

Published online by Cambridge University Press:  18 January 2018

Eleonora Lalli ,
Giulio C. Sarti  and
Cristiana Boi
Eleonora Lalli
Affiliation:
Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Alma Mater Studiorum-Università di Bologna, via Terracini 28, 40131 Bologna, Italy
Giulio C. Sarti
Affiliation:
Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Alma Mater Studiorum-Università di Bologna, via Terracini 28, 40131 Bologna, Italy
Cristiana Boi*
Affiliation:
Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Alma Mater Studiorum-Università di Bologna, via Terracini 28, 40131 Bologna, Italy
*
Address all correspondence to Cristiana Boi at [email protected]
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Abstract

The preparation, screening, and characterization of affinity membranes require a deep knowledge of the behavior of all components of the affinity material. Several studies report the effect of different spacers in combination with the ligand molecule, but the effect of the spacer arm “per se” is generally disregarded. The effect of the spacer 1,2-diaminoethane on non-specific protein adsorption was recently investigated and the results were compared with the ones obtained with A2P affinity membranes. The results show that this spacer has indeed an important effect and that similar specific studies need to be performed for every spacer molecule.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 1 , March 2018 , pp. 65 - 70
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Copyright
Copyright © Materials Research Society 2018 

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