The seasonal organization patterns of demersal assemblages (fish and cephalopods) in the Gulf of Lions were investigated on the basis of a set of bottom trawl surveys. The use of Partial Triadic Analysis (PTA) provided a detailed representation of: (1) the common part of the spatial organization of demersal assemblages at seasonal scale; and (2) the seasonal variability of each species around this common structure. During the course of the study, demersal assemblages exhibited a strong seasonal stability in their organization patterns and only few species showed a strong seasonal variation in their spatial distribution. Demersal assemblages of the Gulf of Lions were structured primarily on the basis of a non depth coast–open sea gradient, which occurred from the coast to the midshelf region. The influence of depth on the structure of fish assemblages appeared to be only of limited importance and mainly restricted to the continental slope area. The PTA combined with contouring technics could be used to improve our knowledge of community structuring factors, and offers a theoretical framework to assess the reproducibility of multispecies structures.