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Single-pot biofabrication of living fibers for tissue engineering applications

Published online by Cambridge University Press:  01 June 2018

Paulomi Ghosh ,
Arun Prabhu Rameshbabu ,
Dipankar Das ,
Bhuvaneshwaran Subramanian ,
Sintu Kumar Samanta ,
Sabyasachi Roy ,
Sagar Pal ,
Sudip Kumar Ghosh  and
Santanu Dhara
Paulomi Ghosh*
Affiliation:
Biomaterials and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India; and CSIR–Indian Institute of Chemical Biology, Kolkata, West Bengal–700032, India
Arun Prabhu Rameshbabu
Affiliation:
Biomaterials and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
Dipankar Das
Affiliation:
Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad–826004, India
Bhuvaneshwaran Subramanian
Affiliation:
Biomaterials and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
Sintu Kumar Samanta
Affiliation:
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
Sabyasachi Roy
Affiliation:
Department of Gynaecology, Midnapore Medical College, Paschim Medinipur–721101, India
Sagar Pal
Affiliation:
Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad–826004, India; and Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
Sudip Kumar Ghosh*
Affiliation:
Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad–826004, India; and Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
Santanu Dhara*
Affiliation:
Biomaterials and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur–721302, India
*
a)Address all correspondence to these authors. e-mail: [email protected]
b)e-mail: [email protected]
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Abstract

In this work, morphology, viability, and metabolism of the amniotic mesenchymal stem cells conditioned with different citric acid (CA)/media ratios were investigated using rhodamine-phalloidin/4′,6-diamidino-2-phenylindole staining, live/dead assay, proliferating cell nuclear antigen, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay). The cells cultured in 25:75 CA/media displayed well spread actin filaments with a prominent nucleus and evidenced optimum viability. The gelation kinetics of chitosan solution in CA/media (25:75) was monitored via dynamic time sweep analysis on a rheometer. The chemical cross-linking of chitosan with CA was confirmed by nuclear magnetic resonance studies. Subsequently, chitosan solution was extruded in CA/media bath containing cells under benign conditions to form cell-laden fibers (living fibers). The prelabeled cells imaged immediately after fiber formation confirmed the attachment of the cells on the fibers. This approach has several advantages including instantaneous gelation, tunable mechanical properties, and adjustable biodegradability that can provide a platform technology for creating viable three dimensional (3D) building blocks for tissue engineering applications.

Keywords

biomaterialpolymer
Type
Invited Article
Information
Journal of Materials Research , Volume 33 , Issue 14: Focus Issue: 3D Printing of Biomaterials , 27 July 2018 , pp. 2019 - 2028
Copyright
Copyright © Materials Research Society 2018 

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Footnotes

c)

These authors contributed equally to this work.

References

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