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A novel, green, and biocompatible graphene-based carbonaceous material for immobilization of cytochrome c

Published online by Cambridge University Press:  09 November 2018

Kuo Gai ,
Maoping Kang ,
Qian Huang ,
Sainan Zheng ,
Lei Zhang ,
Chaoliang Zhang  and
Liying Hao Open the ORCID record for Liying Hao [Opens in a new window]
Kuo Gai
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Maoping Kang
Affiliation:
Department of Mining Engineering, Shanxi Institute of Energy, Taiyuan 030600, People’s Republic of China
Qian Huang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Sainan Zheng
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Lei Zhang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Chaoliang Zhang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Liying Hao*
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

The immobilization of cytochrome c (cyt c) on tea polyphenol functionalized and reduced graphene oxide (TPG) was carried out by a simple adsorption process. Intriguingly, TPG with large surface area exhibited excellent adsorption behaviors and good biocompatibility. The adsorbed materials were characterized by various methods including scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). And the effects of adsorption behavior of cyt c were discussed in detail. The results showed the adsorption behavior was dependent on the pH value and showed a high adsorption capacity as high as 1.414 × 104 mg/g and was friendly to normal cells (mouse fibroblast cell line, L929). In conclusion, we proposed the introduction of TPG as novel material and used the adsorption method to immobilize cyt c, which would provide a novel material and simple method for the enrichment of protein.

Keywords

TPGcytochrome cadsorption
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 24 , 28 December 2018 , pp. 4270 - 4277
Copyright
Copyright © Materials Research Society 2018 

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Footnotes

b)

These authors contributed equally to this work.

References

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