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Purification and partial characterization of proteinase activity in eggs of Dicrocoelium dendriticum

Published online by Cambridge University Press:  12 April 2024

C. Giménez-Pardo ,
C. de Armas-Serra  and
F. Rodríguez-Caabeiro
C. Giménez-Pardo*
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
C. de Armas-Serra
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
F. Rodríguez-Caabeiro
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
*
*Fax: +34 1 885 46 63 E-mail: [email protected]
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Abstract

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A preliminary purification has been carried out by continuous elution electrophoresis of a 49.5 kDa protease of crude extracts from Dicrocoelium dendriticum eggs. The enzyme showed a high capacity to degrade the collagen derivative azocoll at acidic pH. Although it is necessary to carry out further experiments to confirm any physiological role, this protease could be implicated in penetration mechanisms.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 1 , January 2001 , pp. 57 - 59
Copyright
Copyright © Cambridge University Press 2001

References

Armas-Serra, C. de., Giménez-Pardo, C. & Rodríguez-Caabeiro, F. (1993) Presence of proteolytic enzymes in Dicrocoelium dendriticum eggs. International Journal for Parasitology 23, 681–684.CrossRefGoogle Scholar
Armas-Serra, C. de, Giménez-Pardo, C., Jiménez-González, A., Bernadina, W.E. & Rodriguez-Caabeiro, F. (1995) Purification and preliminary characterization of a protease from the excretion-secretion products of Trichinella spiralis muscle-stage larvae. Veterinary Parasitology 59, 157–168.CrossRefGoogle ScholarPubMed
Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Criado-Fornelio, A., Armas-Serra, C. de, Gimenez-Pardo, C., Casado-Escribano, N., Jiménez-González, A. & Rodriguez-Caabeiro, F. (1992) Proteolytic enzymes from Trichinella spiralis larvae. Veterinary Parasitology 45, 133–140.CrossRefGoogle ScholarPubMed
Hunter, C., Lockwood, B.C. & Coombs, G.M. (1992) Hydrolysis of L-leucine methyl ester by Leishmania mexicana mexicana amastigote cysteine proteinases. International Journal for Parasitology 22, 711–717.CrossRefGoogle ScholarPubMed
McKerrow, J.H. & Doenhoff, M.J. (1988) Schistosoma proteases. Parasitology Today 4, 334–340.CrossRefGoogle Scholar
Monroy, F.G., Cayzer, C.J.R., Adams, J.H. & Dobson, C. (1989) Proteolytic enzymes in excretory/secretory products from adult Nematospiroides dubius . International Journal of Parasitology 19, 129–131.CrossRefGoogle ScholarPubMed
Pino-Heiss, S., Brown, M. & McKerrow, J.H. (1985) Schistosoma mansoni: degradation of host extracellular matrix by eggs and miracidia. Experimental Parasitology 59, 217–221.CrossRefGoogle ScholarPubMed
Sung, C.K. & Dresden, M.H. (1986) Cysteinyl proteinases of Schistosoma mansoni eggs: purification and partial characterization. Journal of Parasitology 72, 891–900.CrossRefGoogle ScholarPubMed
Vazquez-López, C., Armas-Serra, C., de Pérez-Serrano, J., Giménez-Pardo, C. & RodrÍguez-Caabeiro, F. (2000) Localization of a 24-kDa collagenase in the Gymnorhynchus gigas plerocercoid. Journal of Helminthology 74, 183–187.CrossRefGoogle ScholarPubMed
Weber, K. & Osborn, M. (1969) The reliability of molecular weight determinations by dedecyl-sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 224, 4406–4412.CrossRefGoogle Scholar
Yoshino, T.P., Lodes, M.J., Rege, A.A. & Chapell, C.L. (1993) Proteinase activity in miracidia transformation excretory-secretory products and primary sporocysts of Schistosoma mansoni . Journal of Parasitology 79, 23–31.CrossRefGoogle ScholarPubMed