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In vitro effects of isoprinosine and a dipeptide methyl ester on Echinococcus multilocularis protoscoleces

Published online by Cambridge University Press:  12 April 2024

P. Lawton ,
N. Walchshofer  and
M.E. Sarciron
P. Lawton*
Affiliation:
Département de Parasitologie et Mycologie Médicale
N. Walchshofer
Affiliation:
Laboratoire de Chimie Organique, Faculté de Pharmacie, 8 avenue Rockefeller, F-69373 Lyon Cedex 08, France
M.E. Sarciron
Affiliation:
Département de Parasitologie et Mycologie Médicale
*
*Fax: +33 4 78 77 71 58 E-mail: [email protected]
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Abstract

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A protoscoleces/vesicles in vitro maintenance test with assessment of viability by eosin exclusion was used to evaluate the quantitative and qualitative activities of isoprinosine, its active component inosine and the dipeptide methylester L-Phe-Phe-OMe on isolated protoscoleces of Echinococcus multilocularis for 24 and 48 h. Isoprinosine and inosine showed dose- and time-dependent activity, the latter displaying a more rapid effect than the former. A high activity was shown with L-Phe-Phe-OMe, when compared to praziquantel. Ultrastructural alterations were much more striking with L-Phe-Phe-OMe, with an effect similar to that of praziquantel, whereas the chemotherapeutic activity of inosine and isoprinosine appeared to be directed against a metabolic target, with a lethal effect not immediately visible at the ultrastructural level. Thus, the previously reported in vivo activities of these drugs result largely from a direct effect on the parasite.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 3 , September 2001 , pp. 251 - 257
Copyright
Copyright © Cambridge University Press 2001

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