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Comparison of the antigenicity of protoscoleces and microvesicles of Echinococcus multilocularis prepared from rats

Published online by Cambridge University Press:  12 April 2024

A. Ito ,
T. Kanazawa ,
M. Nakao ,
Y. Sako ,
Y. Ishikawa  and
K. Nakaya
A. Ito*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
T. Kanazawa
Affiliation:
Department of Tropical Medicine and Parasitology, University of Occupational and Environmental Health, Japan, Kita-Kyushu 807-8555, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
Y. Sako
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
Y. Ishikawa
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa 078-8510, Japan
*
*Fax: +81 166 68 2429 E-mail: [email protected]
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Abstract

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Rats are known to be relatively resistant to infection with Echinococcus multilocularis. However, when rats are inoculated with the parasite tissues, E. multilocularis proliferates slowly at first but after 6 months the cysts increase in size considerably and contain large numbers of protoscoleces. As rats survive for 18 months or longer, approximately 100 ml of packed protoscoleces can be produced from each rat. A comparison of the antigenicity of the protoscoleces and microvesicles by immunoblot methods showed that both Em18 and Em16 are shared components between both protoscoleces and microvesicles, although the latter have some additional antigenic components. In antigens prepared from protoscoleces, the banding patterns around Em18 were much simpler than those from microvesicles. Therefore, for serodiagnosis of E. multilocularis, antigens should be carefully prepared from protoscoleces rather than microvesicles from the rat.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 4 , December 2001 , pp. 355 - 358
Copyright
Copyright © Cambridge University Press 2001

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