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Comparison of the antigenicity of protoscoleces and microvesicles of Echinococcus multilocularis prepared from rats

Published online by Cambridge University Press:  12 April 2024

A. Ito ,
T. Kanazawa ,
M. Nakao ,
Y. Sako ,
Y. Ishikawa  and
K. Nakaya
A. Ito*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
T. Kanazawa
Affiliation:
Department of Tropical Medicine and Parasitology, University of Occupational and Environmental Health, Japan, Kita-Kyushu 807-8555, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
Y. Sako
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
Y. Ishikawa
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa 078-8510, Japan
*
*Fax: +81 166 68 2429 E-mail: [email protected]
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Abstract

Rats are known to be relatively resistant to infection with Echinococcus multilocularis. However, when rats are inoculated with the parasite tissues, E. multilocularis proliferates slowly at first but after 6 months the cysts increase in size considerably and contain large numbers of protoscoleces. As rats survive for 18 months or longer, approximately 100 ml of packed protoscoleces can be produced from each rat. A comparison of the antigenicity of the protoscoleces and microvesicles by immunoblot methods showed that both Em18 and Em16 are shared components between both protoscoleces and microvesicles, although the latter have some additional antigenic components. In antigens prepared from protoscoleces, the banding patterns around Em18 were much simpler than those from microvesicles. Therefore, for serodiagnosis of E. multilocularis, antigens should be carefully prepared from protoscoleces rather than microvesicles from the rat.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 4 , December 2001 , pp. 355 - 358
Copyright
Copyright © Cambridge University Press 2001

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References

Craig, P.S., Rogan, M.T. & Allan, J.C. (1996) Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Advances in Parasitology 38, 169–250.CrossRefGoogle Scholar
Craig, P.S., Deshan, L., Macpherson, C.N.L., Dazhong, S., Reynold, D., Barnish, G., Gottstein, B. & Zhirong, W. (1992) A large focus of human alveolar echinococcosis in central China. Lancet 340, 826–831.CrossRefGoogle Scholar
Craig, P.S., Giraudoux, P., Shi, D., Bartholomot, B., Barnish, G., Delattre, P., Quere, J.P., Harrage, S., Bao, G., Wang, Y., Lu, F. Ito, A., Vuitton, D.A. (2000) An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China. Acta Tropica 77, 167–177.CrossRefGoogle Scholar
Gottstein, B., Jackquir, P., Bresson-Hadni, S. & Eckert, J. (1993) Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen. Journal of Clinical Microbiology 31, 373–376.CrossRefGoogle ScholarPubMed
Ito, A., Nakao, M., Kutsumi, H., Lightowlers, M.W., Itoh, M. & Sato, S. (1993) Serodiagnosis of alveolar hydatid disease by Western blotting. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 170–172.CrossRefGoogle ScholarPubMed
Ito, A., Okamoto, M., Kariwa, H., Ishiguro, T., Hashimoto, A. & Nakao, M. (1996) Antibody responses against Echinococcus multilocularis antigens in naturally infected Rattus norvegicus . Journal of Helminthology 70, 355–357.CrossRefGoogle ScholarPubMed
Ito, A. Ma, L., Paul, M., Stefaniak, J., Pawlowski, Z.S. (1998) Evaluation of Em18- Em16-, antigen B-Western blots, Em2plus-ELISA and four other tests for differential serodiagnosis of alveolar and cystic echinococcosis patients in Poland. Parasitology International 47, 95–99.CrossRefGoogle Scholar
Ito, A., Ma, L., Schantz, P.M., Gottstein, B., Liu, Y.H., Chai, J.J., Abdel-Hafez, A.K., Altintas, N., Joshi, D.D., Lightowlers, M.W., Pawlowski, Z.S. (1999) Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (Em18). American Journal of Tropical Medicine and Hygiene 60, 188–192.CrossRefGoogle Scholar
Kanazawa, T., Asahi, H. & Mochida, K. (1995) Simple techiniques for preparation of small vesicles from Echinococcus multilocularis metacestodes and colorimetric quantitation of the viability of germinal cells. Japanese Journal of Parasitology 44, 441–446.Google Scholar
Ma, L., Ito, A., Liu, Y.H., Wang, X.G., Yao, Y.Q., Yu, D.G. Chen, Y.T. (1997) Alveolar echinococcosis: Em2plus-ELISA and Em18-Western blots for follow-up after treatment with albendazole. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 476–478.CrossRefGoogle Scholar
Nakaya, K., Nakao, M. & Ito, A. (1997) Echinococcus multilocularis: mouse strain difference in hydatid development. Journal of Helminthology 71, 53–56.CrossRefGoogle ScholarPubMed
Nirmalan, N. & Craig, P.S. (1997) Immunoblot evaluation of the species-specificity of Em18 and Em16 antigens for serodiagnosis of human alveolar echinococcosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 484–486.CrossRefGoogle Scholar
Schantz, P.M., Chai, J.J., Craig, P.S., Eckert, J., Jenkins, D.J., Macpherson, C.N.L., Thakur, A. (1995) Epidemiology and control of hydatid disease. pp. 233–331 in Thompson, R.C.A. & Lymbery, A.J. (Eds.) Echinococcus and hydatid disease. Wallingford, Oxon, CAB International.Google Scholar