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Separation of the submicelles from micellar casein by high performance gel chromatography on a TSK-GEL G4000SW column

Published online by Cambridge University Press:  01 June 2009

Tomotada Ono ,
Satoshi Odagiri  and
Toshio Takagi
Tomotada Ono
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate 020, Japan
Satoshi Odagiri
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate 020, Japan
Toshio Takagi
Affiliation:
Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan
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Summary

Bovine caseins from skim-milk (micellar casein and acid precipitated casein) were separated into 4 discrete components by high performance gel chromatography on a TSK-GEL G4000SW columm. The resolution was better than that obtained by conventional gel chromatography which resulted in separation into 2 components at best. Our results showed that the second and third components consisted of αs1- and κ-caseins and αs1- and β-caseins respectively. They are presumed to be submicellar fractions. Samples prepared by removal of Ca phosphate from large and small micelles were rich in the third and the second components respectively. It is suggested that casein micelles consist of αs1- and κ-casein complexes and complexes and polymers of αs1- and β-casein which are situated preferentially on the periphery and in the interior of the micelles respectively.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 1 , February 1983 , pp. 37 - 44
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

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