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Purification and identification of TGF-β2-related growth factor from bovine colostrum

Published online by Cambridge University Press:  01 June 2009

Yukiko Tokuyama  and
Haruhiko Tokuyama
Yukiko Tokuyama
Affiliation:
Department of Nuclear Medicine, Faculty of Medicine, Kanazawa University, 13–1 Takaramachi, Kanazawa, Ishikawa-ken 920, Japan
Haruhiko Tokuyama
Affiliation:
Department of Molecular Immunology, Cancer Research Institute, Kanazawa University, 13–1 Takaramachi, Kanazawa, Ishikawa-ken 920, Japan
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Summary

Bovine colostrum contains transforming growth factor (TGF)-β-like activity. High levels of this activity are found in early colostrum (within 12 h after parturition); however, it decreases rapidly after 30 h. In this study, the activity in early colostrum was purified by a sequence of decaseination, DEAE-Sephacel chromatography, Sephadex G-100 gel filtration, precipitation at neutral pH, further gel filtration and reversed-phase FPLC. A homodimeric 23 kDa protein was identified that retained the TGF-β-like activity. Its activity was completely neutralized by a specific anti-TGF-β2 antibody. These results indicate that the TGF-β-like growth factor found in bovine colostrum is chemically and antigenically related to TGF-β2. The physiological roles of the TGF-β2-related growth factor are discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 1 , February 1993 , pp. 99 - 109
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

REFERENCES

Altman, D. J., Schneider, S. L., Thompson, D. A., Cheng, H.-L. & Tomasi, T. B. 1990 A transforming growth factor β2 (TCF-β2)-like immunosuppressive factor in amniotic fluid and localization of TGF-β2 mRNA in the pregnant uterus. Journal of Experimental Medicine 172 1391–1401CrossRefGoogle Scholar
Assoian, R. K. 1987 Purification of type-β transforming growth factor from human platelets. Methods in Enzymology 146 153–163CrossRefGoogle ScholarPubMed
Assoian, R. K., Komoriya, A., Meyers, C. A., Miller, D. M. & Sporn, M. B. 1983 Transforming growth factor-β in human platelets. Identification of a major storage site, purification, and characterization. Journal of Biological Chemistry 258 7155–7160CrossRefGoogle Scholar
Clark, D. A., Flanders, K. C., Banwatt, D., Millar-Book, W., Manuel, J., Stedronska-Clark, J. & Rowley, B. 1990 Murine pregnancy decidua produces a unique immunosuppressive molecule related to transforming growth factor β-2. Journal of Immunology 144 3008–3014CrossRefGoogle ScholarPubMed
Coffman, R. L., Lebman, D. A. & Shradeh, B. 1989 Transforming growth factor β specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes. Journal of Experimental Mediane 170 1039–1044CrossRefGoogle ScholarPubMed
Daniel, C. W., Silberstein, C. B., Van Horn, K., Strickland, P. & Robinson, S. 1989 TGF-β1-induced inhibition of nousc mammary ductal growth: developmental specificity and characterization. Developmental Biology 135 20–30CrossRefGoogle Scholar
Danielpour, D., Dart, L. L., Flanders, K. C., Roberts, A. B. & Sporn, M. B. 1989 Immunodetection and quantitation of the two forms of transforming growth factor-beta (TGF-β1 and TGF-β2) secreted by cells in culture. Journal of Cellular Physiology 138 79–86CrossRefGoogle Scholar
Danielpour, D., Kim, K. V., Dart, L. L., Watanabe, S., Roberts, A. B. & Sporn, M. B. 1990 Evidence for differential regulation of TGF-β1 and TGF-β2 expression in vivo by sandwich enzyme-linked imimmosorbent assays. Anna's of the New York Academy of Sciences 593 300–305CrossRefGoogle Scholar
Derynck, R., Lindquist, P. B., Lee, A., Wen, D., Tamm, J., Graycar, J. L., Rhee, L., Mason, A. J., Miller, D. A., Coffey, R. J., Moses, H. L. & Chen, E. Y. 1988 A new type of transforming growth factor-β, TGF- β3. EMBO Journal 7 3737–3743CrossRefGoogle Scholar
Espevik, T., Figari, I. S., Ranges, G. E. & Palladino, M. A. 1988 Transforming growth factor-β1 (TGF-β1) and recombinant human tumor necrosis factor-α reciprocally regulate the generation of lymphokine-activated killer cell activity. Comparison between natural porcine platelet-derived TGF-β1 and TGF-β2, and recombinant human TGF-β1. Journal of Immunology 140 2312–2316CrossRefGoogle Scholar
Fontana, A., Frei, K., Boomer, S., Hofer, E., Schreier, M. H., Palladino, M. A. & Zinkbrnagel, R. M. 1989 Transforming growth factor-β inhibits the generation of cytotoxic T cells in virus-infected mice. Journal of Immunology 143 3230–3234CrossRefGoogle ScholarPubMed
Francis, G. L., Upton, F. M., Ballard, F. J., McNeil, K. A. & Wallace, J. C. 1988 Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form. Biochemical Journal 251 95–103CrossRefGoogle ScholarPubMed
Goldman, A. S., Hampono, A. J. & Goldblum, R. M. 1985 Host defenses: development and maternal contributions. Advances in Pediatrics 32 71–100CrossRefGoogle ScholarPubMed
Heremans, J. F. 1974 Immunoglobulin A. In The Antigens, vol. 2, pp. 365–522 (Ed. Sela, M.). New York: Academic PressCrossRefGoogle Scholar
Ho, P. C. & Lawton, J. W. M. 1978 Human colostral cells: phagocytosis and killing of E. coli and C. albicans. Journal of Pediatrics 93 910–915CrossRefGoogle ScholarPubMed
Jin, Y., Cox, D. A., Knecht, R., Raschdorf, F. & Cerletti, N. 1991 Separation, purification, and sequence identification of TGF-β1 and TGF-β2 from bovine milk. Journal of Protein Chemistry 10 565–575CrossRefGoogle Scholar
Kohl, S., Pickering, L. K., Cleary, T. G., Steinmetz, K. D. & Loo, L. S. 1980 Human colostral cytotoxicity. II. Relative defects in colostral leukocyte cytotoxicity and inhibition of peripheral blood leukocyte cytotoxicity by colostrum. Journal of Infectious Diseases 142 884–891CrossRefGoogle ScholarPubMed
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Massagué, J. 1987 The TGF-β family of growth and differentiation factors. Cell 49 437–438CrossRefGoogle ScholarPubMed
Miller, D. A., Lee, A., Pelton, R. W., Chen, E. Y., Moses, H. L. & Derynck, R. 1989 Murine transforming growth factor-β2 cDNA sequence and expression in adult tissues and embryos. Molecular Endocrinology 3 1108–1114CrossRefGoogle Scholar
Noda, K., Umeda, M. & Ono, T. 1984 Transforming growth factor activity in human colostrum. GANN 75 109–112Google ScholarPubMed
Pickering, L. K., Cleary, T. G., Kohl, S. & Getz, S. 1980 Polymorphonuclear leukocytes of human colostrum. 1. Oxidative metabolism and kinetics of killing of radiolabeled Staphylococcus aureus. Journal of Infectious Diseases 142 685–693CrossRefGoogle ScholarPubMed
Roberts, A. B., Frolik, C. A., Anzano, M. A. & Sporn, M. B. 1983 Transforming growth factors from neoplastic and nonneoplastic tissues. Federation Proceedings 42 2621–2626Google ScholarPubMed
Rook, A. H., Kehrl, J. H., Wakefield, L. M., Roberts, A. B., Sporn, M. B., Burlington, D. B., Lane, H. C. & Fauci, A. S. 1986 Effects of transforming growth factor β on the functions of natural killer cells: depressed cytolytic activity and blunting of Interferon responsiveness. Journal of Immunology 136 39163920CrossRefGoogle ScholarPubMed
Shing, Y. W. & Klagsbrun, M. 1984 Human and bovine milk contain different sets of growth factors. Endocrinology 115 273–282CrossRefGoogle ScholarPubMed
Silberstein, G. B., Strickland, P., Coleman, S. & Daniel, C. W. 1990 Epithelium-dependent extracellular matrix synthesis in transforming growth factor-β1-growth-inhibited mouse mammary gland. Journal of Cell Biology 110 2209–2219CrossRefGoogle ScholarPubMed
Sonoda, E., Matsumoto, R., Hitoshi, Y., Ishh, T., Sugimoto, M., Araki, S., Tominaga, A., Yamaguchi, N., & Takatsu, K. 1989 Transforming growth factor β induces IgA production and acts additively with interleukin 5 for IgA production. Journal of Experimental Medicine 170 1415–1420CrossRefGoogle ScholarPubMed
Sporn, M. B., Roberts, A. B., Wakefield, L. M. & De Crombrugghe, B. 1987 Some recent advances in the chemistry and biology of transforming growth factor-beta. Journal of Cell Biology 105 1039–1045CrossRefGoogle ScholarPubMed
Ten Dijke, P., Hansen, P., Iwata, K. K., Pieler, C. & Foulkes, J. G. 1988 Identification of another member of the transforming growth factor type β gene family. Proceedings of the National Academy of Sciences of the USA 85 4715–4719CrossRefGoogle ScholarPubMed
Tokuyama, H. & Tokuyama, Y. 1989 Bovine colostric transforming growth factor-β-like peptide that induces growth inhibition and changes in morphology of human osteogenic sarcoma cells (MG63). Cell Biology International Reports 13 251–258CrossRefGoogle Scholar
Tokuyama, H., Tokuyama, Y. & Migita, S. 1990 a Isolation of two new proteins from bovine colostrum which stimulate epidermal growth factor-dependent colony formation of NRK-49F cells. Growth Factors 3 105–114CrossRefGoogle ScholarPubMed
Tokuyama, H., Tokuyama, Y. & Migita, S. 1990 b Purification of bovine colostric growth factors, BC-1 and BC-2. and their biological functions. Cancer Research Institute Reports, Kanazawa University (1988–1990) 21–22Google Scholar
Tsunawaki, S., Sporn, M. B., Ding, A. & Nathan, C. 1988 Deactivation of macrophages by transforming growth factor-β. Nature 334 260–202CrossRefGoogle ScholarPubMed
Wozney, J. M., Rosen, V., Celeste, A. J., Mitsock, L. M., Whitters, M. J., Kriz, R. W., Hewick, R. M., & Wang, E. A. 1988 Novel regulators of bone formation: molecular clones and activities. Science 242 1528–1534CrossRefGoogle ScholarPubMed
Wrann, M., Boomer, S., De Martin, R., Siepl, C., Hofer-Warbinek, R., Frei, K., Hofer, E. & Fontana, A. 1987 T cell suppressor factor from human glioblastoma cells is a 12–5-kd protein closely related to transforming growth factor-β. EMBO Journal 6 1633–1636CrossRefGoogle ScholarPubMed