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The effect of long term under- and over-feeding on the expression of genes related to glucose metabolism in mammary tissue of sheep

Published online by Cambridge University Press:  23 February 2015

Eleni Tsiplakou ,
Emmanouil Flemetakis ,
Evangelia-Diamanto Kouri ,
Kyriaki Sotirakoglou  and
George Zervas
Eleni Tsiplakou*
Affiliation:
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, GR-11855 Athens, Greece
Emmanouil Flemetakis
Affiliation:
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855 Athens, Greece
Evangelia-Diamanto Kouri
Affiliation:
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855 Athens, Greece
Kyriaki Sotirakoglou
Affiliation:
Department of Mathematics and Statistics, Agricultural University of Athens, Iera Odos 75, GR-11855 Athens, Greece
George Zervas
Affiliation:
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, GR-11855 Athens, Greece
*
*For correspondence; e-mail: [email protected]
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Abstract

Glucose utilisation for lactose synthesis in the mammary gland involves expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, the effect of long term under- and over-feeding on the expression of genes [glucose transporter 1: GLUT1, glucose transporter 3: GLUT3, Sodium glucose contransporter 1: SGLT1, two isoforms of β- (1,4) galactosyltransferase: β- (1,4) GAT1, β- (1,4) GAT3 and α-lactalbumin: LALBA] related to glucose metabolism in sheep mammary tissue (MT) was examined. Twenty-four lactating dairy sheep were divided into three homogenous sub-groups and fed the same ration in quantities which met 70% (underfeeding), 100% (control) and 130% (overfeeding) of their energy and crude protein requirements. The results showed a significant reduction on mRNA of GLUT1 and LALBA gene in the MT of underfed sheep, compared with the respective controls and overfed and a significant reduction on mRNA level of SGLT1 and β- (1,4) GAT1 in the MT of underfed sheep, compared with the overfed ones. A significant increase in the GLUT3 mRNA accumulation in the MT of both under- and over- fed sheep was found. Additionally, a trend of reduction on β- (1,4) GAT3 mRNA level in the MT of the underfed sheep, compared with the overfed, was observed. A close positive relationship was obtained between the mRNA transcripts accumulation of GLUT1, SGLT1, β- (1,4) GAT1 and LALBA gene with the milk lactose content and milk lactose yield respectively. In conclusion, feeding level and consequently nutrient availability, may affect glucose uptake and utilisation in sheep MT by altering the GLUT1, GLUT3, SGLT1, β- (1,4) GAT1 and LALBA gene expression involved in their metabolic pathways.

Keywords

Underfeedingoverfeedingfeeding levelglucose genes expressionsheepmammary gland
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 2 , May 2015 , pp. 228 - 235
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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