A field experiment was made with chickpea (Cicer arietinum L.) at the Indian Agricultural Research Institute, New Delhi during the winters of 1984–5 and 1985–6 to study the effects of plant type and population density on growth and yield of chickpea. Cultivars BG 257, BG 261, BG 268 and BG 274 produced signficantly more grain with 1000 × 103 and 660 × 103 plants/ha, whereas the cultivar BG 209 produced the most at 500 × 103 plants/ha. Among the cultivars, BG 257 and BG 268 gave highest grain yield with 660 × 103 plants/ha.

To study the response of three sugarcane genotypes (CO 1148, COJ 64 and CO 1158) to variations in moisture availability in sandy loam soil (entisol), field trials were conductedat Lucknow (26·5° N, 80·5° E, 120 m altitude) during 1984–5 and 1985–6. Three moisture regimes, i.e. wet (irrigation at 75% available soil moisture (ASM)), moist (irrigation at 50% ASM) and dry (irrigation at 25% ASM) were maintained during the pre-monsoon (before June) period in spring-planted (February-March) sugarcane. During the summer months (until June)the variety CO 1148 had a significantly greater sheath moisture percentage than COJ 64 and CO 1158. Under stress conditions, leaf area index was reduced most in COJ 64 and least in CO 1148.Underground shoots and roots grew faster in CO 1148, and the growth of above-ground parts was quicker in COJ 64. Compared with the 75% ASM regime the reduction in cane yield in the 25% regime was more in COJ 64 and CO 1158 (31 t/ha) than in CO 1148 (12 t/ha). The water requirement of COJ 64 was greater than that of the other varieties. Therefore, for higheryields COJ 64 needed frequent irrigation whereas CO 1148 performed well even under moderate irrigation (50% ASM).

This paper re-analyses three experiments for which results have previously been published. A simpler model for aggression, based only on the proportions of the component yields of the mixed plots, is adopted. This greatly simplifies the analysis, which requires only a straightforward application of least squares, and gives much more accurate estimates of competitive power. Omission of some combinations from the mixtures included in an experiment presents little extra difficulty.

Various secondary matters, some of which have implications for the design of experiments of this type, are discussed in the paper. Graphical plots which throw light on the effect of sole yield differences on competitive power are exhibited. A logarithmic transformation of the data, often used to equalize the variance, was found to lead to underestimation of enhancement.

Gestational age-related changes in plasma free thyroxine (FT4) concentration in maternal and foetal goats were determined for 65–140 days of pregnancy. The placental permeability to maternal thyroxine (T4) and iodine (I) during the last trimester of pregnancy was evaluated.

The presence of T4 in foetal circulation at 65 days' gestation, the absence of a significant correlation between maternal and foetal levels and the absence of any transplacental diffusion of 125I–T4 showed autonomous foetal thyroid function during pregnancy in goats. Significantly low maternal FT4 was measured at 95 and 110 days' gestation (32·7 and 31·5 pmol/1 respectively). Plasma concentration increased to 48·6 pmol/1 at day 125 then fell before parturition to a level similar to that of non-pregnant goats (41·9 pmol/1). In contrast, foetal FT4 increased with gestational age to 110 and 125 days when levels of 64·0 and 59·1 pmol/1 respectively were greater than those at all other ages, suggesting that the foetal hypothalamic-pituitary-thyroid is functioning at a higher rate than the maternal system around this time. This was supported by increased foetal thyroid affinity for I when compared with the maternal thyroid at 110–140 days' gestation.

A significant cubic relationship between foetal FT4 and gestational age was established.

Particulate fractions prepared from meadow hay, ranging in size from 0·1 to 2 mm, were incubated with rumen digesta from four cannulated Romney sheep fed the same hay. The rates of gas production, lipolysis of corn oil and hydrogenation of linoleic acid were measured.

The rate of gas production per g fermentable particles (FP) was approximately 30% lower with 1–2 mm than with the 0·1–0·4 mm particles. However, per m2 surface area the rate for the larger particles was found to be approximately 600% greater.

The rates of lipolysis of triacylglycerols and hydrogenation of linoleic acid were respectively 25 and 60% higher per g FP and 1100 and 1200% higher per m2 FP surface area with the 1–2 mm particulate fraction.

The same hay particulate fractions were incubated with pure cultures of Ruminococcus flavefaciens, since this organism is active in both lipid metabolism and cellulose fermentation. The rate of gas production and the number of organisms adhering to the particles were determined.

The effects of particle size on gas production were similar to those found when incubations were carried out with rumen digesta. Per g FP the rate was 40% lower with 1–2 mm than with 0·1–0·4 mm particles. However, per m2 surface area the rate was found to be approximately 450% greater with the former.

It was further found that although the density of the bacterial population on 1–2 mm particles was 600% higher than on the 0·1–0·4 mm particles, the rate of gas production per 109 bacteria remained unchanged.

We conclude that per m2 surface area fermentation, lipolysis and hydrogenation were more rapid with particles ranging from 1 to 2 than from 0·1 to 0·4 mm in size. This was due, at least in part, to microbial population density.

A trial to investigate possibilities of berry control by cultivation depth is described. Tubers planted at 10 cm produced significantly more potato plants in flower than those planted at 30 cm at the commencement of flowering. However, 3 weeks later the difference in numbers of flowers had disappeared. Emergence of overwintered tubers was about 53%, and of spring-planted tubers about 97%. Depth of planting did not affect emergence. A comparison of overwintered and spring-planted potato tubers showed earlier flowering and berry production by the overwintered tubers of about 1 week on average compared with the spring-planted tubers.

Six chemical treatments were compared at different concentrations and growth stages of potatoes and peas to assess potential control of berry production in potato volunteers growing in a vining pea crop.

Best control was found to be achieved with a combination of 4-(2-methyl-4-chlorophenoxy) butyric acid (MCPB) at 8300 mg a.i./l and Bentazone at 7300 mg a.i./l applied with the potato volunteers at full foliage to flowering stage, and before the pea crop reached the flowering bud stage, or by application of MCPB alone at 8300 mg a.i./l with the potato plants at full foliage to the pre-flowering stage. No chemical treatment either totally destroyed potato foliage, or completely suppressed potato flower and berry production, but it is argued that growers may reduce the effects considerably by careful observation and use of the appropriate chemical at the appropriate time.

In intermediate deepwater conditions (15–50 cm), direct seeding was superior to transplanting in 20 cm of water. Varieties with greater plant height performed better under transplanted conditions. Nitrogen fertilizer application at 40 kg/ha increased the grain yield under both direct-seeded and transplanted conditions. Though yield of the transplanted crop was less than that of the direct-seeded crop, the decrease was less in the crop given nitrogen fertilizer.

Under conditions of semi-deep water (51–100 cm) rice varieties with greater plant height produced more dry matter and grain yield as N fertilizer was increased from 0 to 20 and 40 kg/ha. The varieties which performed better in a situation of slow rise in water level to a depth of 120 cm could not survive a quick rise in water level owing to their lesser plant height and elongation ability. The loss of dry matter (dead and dried leaves) was also greater in varieties susceptible to deep water than varieties tolerant of deep water.

With the increase in N fertilizer, there was increase in plant height, number of tillers, dry-matter production and grain yield. The loss of dry matter was less in crops given N fertilizer than in those not given N fertilizer. The varieties capable of producing higher grain yield in response to N fertilizer under semi-deep water did not improve their grain yield under intermediate deep water (15–50 cm). With increase in application of N fertilizer from 0 to 40 kg/ha the N concentration in grain increased. N concentration did not vary in straw under intermediate deep water, there was no definite trend in semi-deep water, and it was higher under conditions of semi-deep than intermediate deep water.

The dry-matter digestibilities of 13 important herbaceous and browse forages from an arid rangeland in north-western Kenya were evaluated in a comparative in vitro trial using rumen fluid from indigenous sheep, goats, a camel, and Merino sheep kept at an agricultural laboratory. All inoculum donors had varied diets preceding the experiment, but little substantial difference was observed in the ability of each fluid to digest test materials. Tt was concluded that the Merino sheep inoculum provided an adequate substitute for inocula from indigenous animals for studies of the nutritional ecology of free-ranging livestock.

Relationships between slaughter weight, side weight, linear carcass measurements, carcass joints, carcass composition, and prediction equations for carcass composition have been established for Sudan Desert lambs.

Of the carcass linear measurements, chest depth had the highest coefficient of correlation with slaughter weight (r = 0·62) and side weight (r = 0·64) followed by the circumference of the buttocks with correlation coefficients of 0·49 and 0·63 respectively.

Correlation coefficients between percentage tissue in the side and percentage tissue in the different joints showed that percentage lean in middle neck and shoulder, leg and best end, in this order were strongly associated with percentage lean in side (P < 0·001). Percentage bone in middle neck and shoulder, leg and loin was positively associated with percentage bone in the side. Fat in leg and best end neck gave a significant correlation (P < 0·001) with percentage fat in the side.

Trivariate regression equations for prediction of carcass composition were calculated for both sexes in the two breed types and the best end neck joint was found to be the best practical predictor with R2a (adjusted coefficient of determination) of 0·81–0·98 for lean prediction and 0·70–0·92 for fat prediction.

The phenotype of medium duration pearl millet varieties grown in West Africa differs from that of the shorter duration millets grown in India. African varieties are usually much taller, have longer panicles, fewer productive tillers, and a lower ratio of grain to above-ground dry-matter (harvest index). The effect of crop duration on plant phenotype was investigated in two hybrids using extended daylengths to increase the duration of the vegetative phase (GSl: sowing to panicle initiation). The two hybrids, 841A × J104 and 81A × Souna B, were considered to represent the Indian and African phenotype, respectively. Tiller production and survival, leaf area, and dry-matter accumulation and partition, were monitored over the season. Grain yield and its components were determined at maturity.

The two hybrids responded similarly to the short and long daylength treatments. The duration of GSl was increased from 20 to 30 days, resulting in increased number of leaves, leaf area, and stem and total dry-matter accumulation; there was no effect on tiller production and survival, or on panicle growth rate. Grain yield was, therefore, the same in both GSl treatments, and harvest index (HI) was much reduced in the long GSl treatment owing to the increased stem growth. One evident effect of a longer GSl was on dry-matter partitioning between shoots; partitioning to the main stem (MS) was increased, whereas partitioning to the tillers was reduced.

There was no difference in crop development, growth or yield between the two hybrids in either GSl treatment. The only significant differences were in the efficiency with which intercepted radiation was converted to dry matter, which was greater in 841A × J104 than in 81A × Souna B, and in the balance between MS and tillers; the grain yield of the MS was significantly greater in 81A x Souna B than in 841A × J104, but at the expense of number of productive tillers.

The results demonstrate that both African and Indian phenotypes are equally productive under good agronomic conditions. The lower HI in longer duration African millets is a consequence of a much extended stem growth phase and therefore increased competition between stem and panicle during grain filling. Possible ways to increase grain yield in the medium duration African millets are considered.

Six nitrosoguanidine-induced manganese-resistant mutants of Azospirillum brasilense were isolated and used for associative N2 fixation studies with cheena (Panicum miliaceum L.) in acid soil. Three mutants showed cross-resistance to aluminium and neomycin, but of these three only one showed cross-resistance to streptomycin. Inoculation of cheena with A. brasilense and its mutants at different rates of applied nitrogen led to a significant increase in associative N2 fixation, N content, dry-matter production and grain yield in acid soil. A saving of about 20 kg N/ha could be achieved by inoculating seeds of cheena with these mutant strains in acid soil.

The relationship between germination rate within a carrot seed lot and subsequent seedling performance was studied in four seed lots of different viability. Seedling performance was assessed using slope tests conducted under controlled conditions and in the field following fluid drilling under a range of environmental conditions. Germination rate within a seed lot was positively related to vigour measured by slope tests, percentage emergence and seedling weight, and was negatively related to the spread of emergence times (In variance days) and the coefficient of variation (c.v.) of seedling weights. Sowing seeds selected as fast germinating resulted in greater seedling emergence percentages over a range of environmental conditions than when sowing seeds selected as slowly germinating.

The percentage (P) of incident photosynthetically active radiation (PAR) reaching the ground was measured in winter wheat crops throughout the season, and related to projected area index (L) of leaves, plus sheaths and stems when present. Between unfolding of the first leaf and appearance of the flag leaf the mean value for the exponential extinction coefficient (K) was 0·46±0·007. The value did not vary with the growth stage of the wheat and was scarcely affected by experimental treatments, apart from effects resulting from the presence of senescent tissues that intercepted light but whose area was not included in L. After anthesis, in crops having between 340 and 540 ears/m2, between 68 and 89% of incident PAR penetrated to below the ears. Relation between the further penetration to the base of the crop canopy and the area of green plus senescent leaf and stem tissue gave K = 0·50. The relation did not change significantly between anthesis and complete senescence.

The relation between P and L was similar whether P was obtained from instantaneous measurements made near midday, 24 h integrals of PAR or percentage ground cover measured photographically.

Development was studied in winter wheat sown after rape or after oats in September 1984, 1985 and 1986, and in October 1985. N03-N residues from rape in October exceeded those from oats by 60, 50 and 40 kg N/ha respectively in the 3 years. Previous crops affected development only in the 1984–5 season. The first 12 apical primordia were initiated faster after rape; thereafter rates were similar, wheat after rape having two more primordia than wheat after oats. Final numbers of leaves and spikelets were each increased by one. The first five leaves appeared faster after rape so that from December onwards the number of emerged leaves was one more after rape than after oats. The double ridge and terminal spikelet stages occurred 8 and 4 days respectively earlier after rape than after oats. Development was unaffected by N fertilizer applied in November or at various times from February onwards, although growth and N uptake were increased. Drought during autumn 1985 delayed emergence of the September-sown wheat and slowed the rates of initiation of the first ten primordia and appearance of the first four leaves. Consequently the number of shoots per plant in December was only about three cf. six in the other 2 years.

Winter wheat grown following potatoes on a sandy loam at Woburn in 1978–9, 1980–1 and 1981–2 was compared with that on a clay loam at Rothamsted in 1978–9 and 1980–1, and on a silty clay (alluvium) at Woburn in 1981–2. The cultivar was Hustler in the harvest years 1979 and 1981 and Avalon in 1982. On each soil in each year multifactorial experiments tested effects of combinations of six factors, each at two levels.

The best 4-plot mean grain yield ranged from 89 to 11·1 t/ha during the 3 years; it was smaller on the sandy soil than on the clay soil in 1979, but larger on sand than on the clay in 1981 and 1982. Until anthesis the number of shoots, dry weight and N content of the wheat giving these best yields were less on sand than on clay. Unlike grain weight, straw weight was always less on sand.

Sowing in mid-September instead of mid-October increased grain yield on clay in each year (by 0·4·0·7 t/ha) and increased yield on sand only in 1981 (by 1·6 t/ha). Early sowing always increased dry weight, leaf area, number of shoots and N uptake until May. The benefits were always greater on clay than on sand immediately before N fertilizer was applied in the spring and usually lessened later on both soils.

Aldicarb as an autumn pesticide increased grain yield of early-sown wheat on both soils in 1981 by lessening infection with barley yellow dwarf virus. Aldicarb increased yield on clay in 1982; it also decreased the number of plant parasitic nematodes.

Wheat on sand was more responsive to nitrogen in division, timing and amount than was wheat on clay. In 1979 yield of wheat on sand was increased by dividing spring N between March, April and May, instead of giving it all in April, and in 1982 by giving winter N early in February. In 1981 division and timing on sand interacted with sowing date. Yield of early-sown wheat given N late, i.e. in March, April and May, exceeded that given N early, i.e. in February, March and May, by 1·4 t/ha; single dressings given all in March or all in April also yielded less than the late divided dressing. Yield of later-sown wheat given all the N in April was at least 1·2 t/ha less than with all N given in March or with divided N. In all years treatments that increased yield usually also increased N uptake. Grain yield on clay was never affected by division or timing of spring N or by application of winter N. This was despite the fact that all treatments that involved a delay in the application of N depressed growth and N uptake in spring on both sand and clay. The mean advantage in N uptake following early application of spring N eventually reversed on both soils, so that uptake at maturity was greater from late than from early application. Increasing the amount of N given in spring from the estimated requirement for 9 t/ha grain yield to that for 12 t/ha increased yield in 1982, especially on sand. The larger amount of N always increased the number of ears but often decreased the number of grains per ear and the size of individual grains.

Irrigation increased grain yield only on the sandy soil, by 1·1 t/ha in 1979 and by 07 t/ha in 1981 and 1982. The component responsible was dry weight per grain in 1979 and 1982, when soil moisture deficits reaching maximum values of 136 and 110 mm respectively in the 2 years developed after anthesis; the component responsible was number of ears/m2 in 1982 when the maximum deficit of 76 mm occurred earlier, in late May.

Field experiments were conducted at the Punjab Agricultural University, Ludhiana from 1980–1 to 1984–5 to study the response of lentil genotypes to phosphorus application and Rhizobium inoculation. The number and dry weight of nodules increased consistently with increasing rates of application of phosphorus from 0 to 60 kg P2O5/ha. Nitrogenase activity of intact root nodules increased from 17 530 to 22 390 nmol/h per g dry weight of nodules with 20 kg P2O6/ha and to 27391 and 29170 nmol/h per g with 40 and 60 kg P2O5/ha, respectively. Rhizobium inoculation also increased nodulation, nitrogenase activity and grain yield. Interaction between phosphorus and Rhizobium inoculation was significant in 3 out of 5 years, indicating that the combination of Rhizobium and 20 kg P2O6/ha gave yield equivalent to 40 kg P2O6/ha without Rhizobium.

The expression of symptoms of stem nematode reproduction on a total of 53 white clovers (26 cultivars, 14 genepools and 13 introductions from plant collections) was studied in a series of field and glasshouse experiments. Seedlings or stolon-tip cuttings were inoculated with nematodes and the clovers classified by the proportion of plants which developed symptoms. Significant differences were found between varieties although in each test the majority was intermediate between more resistant and susceptible extremes. There was significant positive correlation between tests, in spite of different inoculation methods and different average levels of susceptibility. Very large-leaved cv. Aran was more resistant than most other clovers tested, and small-leaved cv. S. 184 was more susceptible. There was no general correlation of leaf size with reaction to stemnematode. Small-leaved cv. Pronitro was also resistant while several large-leaved cultivars were susceptible. In observations of plants exposed to nematodes over a long period, either by sequential inoculations or through perpetuating latent infections, apparently resistant plants eventually succumbed and supported nematode reproduction. It has not been determined whether this was because selection for virulence in the nematodes had occurred.

Two experiments were carried out to examine the value of copper, cobalt and selenium contained in soluble glass boluses as a means of providing supplementary trace elements to goats.

In the first experiment 36 purebred Toggenburg goats were used and divided into two groups. One group was treated with soluble glass boluses while the other was left as control.

In the second experiment 46 Saanen half-bred goats were used, divided into three groups. The first group was treated with soluble glass boluses, the second was treated with selenium injectable compound and the third was left as control.

Blood samples were collected on four occasions, during an experimental period of almost 12 months and plasma copper, serum vitamin B12 and blood selenium concentrations were determined. All those measurements showed significant increases for about a year, and were due to treatment. The advantages of glass boluses were not shared by the selenium injectable compound.

All combinations of four levels of potassium and two of nitrogen were applied in each of 2 years to a hill sward on a brown podzolic soil with an 8 cm peaty surface horizon anda very low level of extractable K. The sward had been surface-sown 11 years before with white clover (Trifolium repens L.) and perennial ryegrass (Lolium perenne L.). During the course of the experiment, harvesting was by cutting, with an average interval between cuts of 5 weeks. The previous management had been sheep grazing.

The proportion of clover in harvested herbage was increased by applied K and reduced by applied N. The clover grew strongly in the 1st year of cutting management where K, but no N, was applied, but there was much less clover in the 2nd year on all plots. The concentration of K in both clover and grass was greatly increased by applied K, but only the clover responded strongly in terms of extra herbage produced. This suggests that grass growth was N-limited, whereas the clover had sufficient N from symbiotic fixation.

Applied K increased the size and weight of clover leaves, the rate of extension of the stolon main axis, and the number and length of stolon branches, but did not affect the rate of emergence of leaves on the stolon main axis. Applied N had a generally negative effect on the aspects of clover growth and morphology which were measured. The length and weight per ryegrass leaf blade were generally slightly increased by both applied K and applied N.