Published online by Cambridge University Press: 27 March 2009
Six nitrosoguanidine-induced manganese-resistant mutants of Azospirillum brasilense were isolated and used for associative N2 fixation studies with cheena (Panicum miliaceum L.) in acid soil. Three mutants showed cross-resistance to aluminium and neomycin, but of these three only one showed cross-resistance to streptomycin. Inoculation of cheena with A. brasilense and its mutants at different rates of applied nitrogen led to a significant increase in associative N2 fixation, N content, dry-matter production and grain yield in acid soil. A saving of about 20 kg N/ha could be achieved by inoculating seeds of cheena with these mutant strains in acid soil.