The nature and availability of phosphorus in long-term fertilized pasture soils was investigated in a series of field trials, which included liming, N fertilizer and cultivation and involved monitoring plant P uptake and changes in topsoil (0–7·5 cm) P fractions for 2 years (1982–83). Liming increased soil organic P mineralization. This was indicated by significant decreases in extractable organic P and concomitant increases in microbial biomass P in the limed soils, although these changes in soil P had no significant effect on pasture yield and P uptake. On the other hand, N fertilizer increased pasture yield and P uptake but had little effect on the amounts of P in the different soil P fractions. In the cultivated soils, increases in plant-available inorganic P were attributed to the release of P during decomposition of plant residues, while the maintenance of fallow conditions decreased amounts of microbial P in these soils.

A series of experiments examining the influences of size and spacing of planted potato tubers (‘seed’ tubers) on tuber yields was conducted at four sites in the UK in 1982–84. A new approach to data analysis was used to estimate the optimum tuber planting density for different ware grades. The optimum density, which maximized returns, decreased with increasing seed tuber weight and ratio of seed-tuber cost to ware value and with lower than average total tuber yields. Changing the required ware grade from 40–65 mm to 40–80 mm had a minimal effect on the optimum tuber planting density.

Optimum tuber planting densities were lower than in published recommendations but this disparity appeared to be accounted for by differences in the number of stems produced by each planted tuber. There was some evidence that, with lower seed-tuber weights, the optimum planting density differed with site and that at optimum economic densities small and medium seed tubers outyielded large seed tubers.

Determinate varieties of Vicia faba are smaller and may require higher plant population densities than their conventional indeterminate counterparts to attain high yields. This hypothesis was examined at the University of Nottingham farm at Sutton Bonington, UK, and at INRA, Dijon, France in 1986 and 1987. The determinate varieties Ticol and TP667 and indeterminate M5.1 and Maris Bead were sown in the spring to give a range of densities of 10–100 plants/m2.

Yields generally increased as population density increased. The optimum density for determinate varieties was higher than for indeterminate varieties. Indeterminate varieties yielded more than determinate varieties at all plant densities, perhaps because of their larger canopy. Seed yields were affected by year and location. Greater yields were associated with heavier seeds and more seeds per pod.

Although several yield components, when expressed per unit area, were significantly correlated with seed yield, their coefficients of determination were never very large. This confirmed the plasticity of yield in field beans.

The effects of year of birth, age of dam, sex of calf, month of birth and weaning age on weaning weights of Mashona calves on six commercial farms in Zimbabwe were estimated by least squares procedures from data of 6365 calves born 1977–85. The least squares mean weight at 205 days among farms ranged from 123 to 159 kg while the linear regression coefficients of weight on age ranged from 0·31 to 0·46 kg. Adjustment for weaning age by the average daily gain from birth to weaning failed to remove the dependence of adjusted weight on age. Sex was the most important source of variation and the mean difference between male and female calves was 12·9 kg. Sex difference was markedly influenced by herd production rate, being greater in herds of above average production.

Weights of calves of 3-, 4- and > 11-year-old dams deviated from those of dams aged 5–10 years (mature) by 14·5, 8·5 and 2·9 kg, respectively. Herd mean influenced the difference between 3-year-old and mature dams, the difference increasing as herd mean declined. Herd-year specific factors seem more appropriate for sex and 3-year-old dams and an annual crop of > 50 calves would confer an advantage to herd-specific over general adjustment factors. On the basis of equality of variances within subclasses, multiplicative factors were superior to additive factors for sex of calf and month of birth; both types of adjustment were suitable for age of dam.

The effects on flower drop and pod set of applications of either the auxin 4-choloroindole, the cytokinin 6-benzylaminopurine (BAP) or gibberellic acid (GA3) to every flower, 1 day before or 1 day after tripping, were studied in broad bean plants grown in a glasshouse in 1988. Control plants shed most of their flowers; pods that did set originated primarily from flowers at proximal positions on the raceme. Most pods set on the lower part of the reproductive portion of the control plants. Chloroindole and GA3 application before tripping had no significant effect on pod-set pattern. However, GA3 applied after tripping significantly enhanced pod set at proximal flowers by 21% but inter-raceme pod set was not significantly altered. Chloroindole applied after tripping significantly increased pod set, particularly at proximal flower positions on the raceme; this treatment increased inter-raceme pod set but the increase was significant only for the basal four racemes and racemes 11, 12 and 13. Application of BAP before or after tripping resulted in almost complete pod set on all racemes; but greater flower drop was observed on the uppermost five racemes of plants treated after tripping than on corresponding racemes of plants treated before tripping. The effects of BAP, chloroindole or GA3 treatment were not due to changes in the synchrony of flower opening. An adequate supply of a cytokinin appeared to be necessary at or before pollination to initiate changes in reproductive organs to prepare them to become net attractors of assimilates from other plant parts. Auxin and gibberellin were only effective in promoting fruit set 24 h after tripping. An adequate supply of auxin, and perhaps of gibberellin, in balance with cytokinin appears to be required after pollination to maintain the flow of adequate assimilates to enable further pod development and for maturation to proceed.

The voluntary food intake, in vivo digestibility and site of nutrient digestion was measured in 12 Polled Dorset ram lambs, from 6 to 24 weeks of age, offered clover hay. Lambs were weaned at 6 weeks of age. Six were fitted with a permanent T-shaped cannula in the abomasum and a temporary rumen catheter during the periods of digesta flow measurement.

Surgical modification of lambs did not influence intake (g/kg live weight (W) per day), in vivo digestibility or marker retention time in the rumen. However, intact lambs grew significantly faster than cannulated lambs (139 v. 109g/day, P < 0·05) apparently because of an overall higher cumulative intake over the whole experimental period rather than any change in efficiency of use of nutrients or maintenance requirement.

Relative to live weight, intake increased rapidly until c. 10 weeks of age and remained relatively constant at c. 37 g DM/kg W per day for the remainder of the experiment. Age had no effect on in vivo digestibility of organic matter (OM) or neutral detergent fibre (NDF) (average 0·69).

The proportion of digestible organic matter apparently digested in the rumen (DOMADR) decreased from 0·63 to 0·47 between 8 and 24 weeks of age whilst intake between these measurement periods increased. Nonammonia N (NAN) flow past the abomasum was similar at all ages in relation to digestible organic matter intake (55·1 g NAN/kg DOMI) but was higher at 16 and 24 weeks of age than at 8 and 12 weeks of age, respectively, in relation to organic matter apparently digested in the rumen (OMADR) (116 and 85 g NAN/kg OMADR, respectively, P < 0·05).

Fractional outflow rate of digesta markers from the rumen, when estimated from abomasal digesta, was significantly higher than when estimated from faeces (103Ru-p 0·121 v. 0·09; 51Cr-EDTA 0·169 v. 0·111/h; P > 0·05).

It was concluded that cannulation did not affect intake and digestion characteristics in these young lambs. The changes in the site of nutrient digestion as the animals aged may have been due to agerelated changes in digestive function but appeared to be associated with the increase in intake that occurred between measurement periods of the cannulated lambs.

In two experiments using cattle implanted with 0 or 300 mg trenbolone acetate and fed restricted diets of low-protein roughage, urea synthesis was measured by plasma dilution of a single intravenous injection of [14C]urea. Urea flow to the gut was calculated as the difference between synthesis and urinary urea output. Because rumen ammonia concentrations were only c. 1 mg/1, it was assumed that urea transfer from the gut to blood was negligible.

In the first experiment, fistulated heifers treated with trenbolone acetate synthesized significantly (P < 0·05) less urea (0·8 v. 1·2 g/h) and had a tendency to excrete less (P = 0·08) urea in urine (0·04 v. 0·14 g/h) and to have a lower (P = 0·07) flow of urea into the gut (0·8 v. 1·1 g/h) than untreated animals. They also had significantly (P < 0·01) lower rumen digestion rates, determined from disappearance of feed dry matter from nylon bags suspended in the rumen for 48 h.

In the second experiment, steers treated with trenbolone acetate synthesized significantly (P < 0·05) less urea (1·0 v. 1·9 g/h) and had a significantly (P < 0·05) lower flow of urea into the gut (0·9 v. 1·8 g/h) than untreated steers.

The results were consistent with an hypothesized sequence of events resulting from treatment with trenbolone acetate. The sequence proposed was a decrease in the rate of tissue protein breakdown, a decrease in the rate of urea synthesis from de-amination of amino acids, a decrease in the amount of urea recycled to the rumen, a decreased rumen digestion rate and thus a decrease in feed intake.

Merino sheep were given abomasal infusions of various amino acids or mixtures of amino acids. Effects on wool growth were measured using autoradiography or a clipping procedure and changes in the concentration of amino acids in plasma were measured in some experiments.

Mixtures of five (28 g/day) or ten (45 g/day) essential amino acids (both mixtures containing 3 g methionine) stimulated wool growth of sheep receiving a maintenance ration; on average, the volume of wool grown increased 48% and 86%, respectively. When cysteine completely replaced methionine in these mixtures, wool growth was markedly reduced, but two-thirds of the methionine could be replaced by cysteine without affecting wool growth. Homocysteine was partially effective in replacing methionine and, when supplemented with betaine, folic acid and vitamin B12, the mixture was still significantly inferior to that containing methionine. In contrast, abomasal supplements of methionine or homocysteine alone were equivalent as supplements for wool growth. The results indicated a specific role for methionine in the control of wool growth, other than the provision of cysteine. This role was postulated to be related to some function of S-adenosylmethionine.

Infusion often essential amino acids caused appreciable increases in the concentrations of cystine, methionine, cystathionine and taurine in plasma; total essential amino acids increased threefold whereas nonessential amino acids decreased in concentration. The replacement of methionine in the infusion by cysteine or homocysteine significantly altered the concentration of cystine, methionine and cystathionine in plasma.

Evidence was obtained that the adverse effects on wool growth of high abomasal doses of methionine (10g/day) could not be reduced or prevented by provision of additional glycine and were not related to the supposed toxic effects of 3-methylthiopropionic acid, a metabolite of the transamination pathway.

Three double-marker systems were used in cannulated steers offered straw-based diets supplemented with barley, fishmeal or a combination of both, in an attempt to reduce the risks of misinterpreting biological results based on calculations of digesta flows using markers. Ruthenium phenanthroline (Ru-phe), ytterbium acetate (Yb-ac) and the indigestible acid detergent fibre fraction (IADF) were used as markers of the particulate phase of digesta in conjunction with Cr-EDTA as a liquid-phase marker to estimate duodenal and ileal digesta flows. The present paper presents the difficulties met in the application of marker techniques.

The particulate marker used in a double-marker system for calculation of nutrient flow modified the degree of statistical significance observed for dietary treatment effects on digesta flow parameters.

The data suggest that the type of diet used affects the behaviour of individual markers in different ways and could incur some bias in the results. It is hypothesized that the physical and chemical characteristics of digesta, in different dietary conditions, can be responsible for differential attachment of markers to particles of the solid phase. In such cases, the distinction made in the double-marker technique between particulate and liquid phases may not be sufficient to correct for lack of representativeness of digesta contents sampled from T-shaped cannulae.

For the diets studied it was concluded that the interpretation of digestion parameters should be based on both Ru-phe and Yb-ac results.

No-tillage and dry ploughing were compared with puddling under identical cultivation conditions for their effect on growth and yield of wet-season rice. The experiment was conducted on an alluvial sandy clay–loam soil over 5 years (1978–82) at Cuttack, India. In the preceding dry season, each plot was split into two halves for growing dry-season crops, with and without tillage. Growth of rice seedlings without applying N to the seed bed was faster and more vigorous after puddling, as shown by greater shoot and root weight per hill and a higher shoot:root ratio, and remained so until maturity. This was reflected in earlier flowering and maturity, followed by greater grain and straw yields. These results are attributed to the ability of the roots to obtain more N from the puddled soil.

Under high water table conditions, extractable and soluble N in the root zone were not much influenced by tillage treatments. Grain and straw yields after no-tillage were similar to those produced by dry ploughing but smaller than those produced by puddling, although the differences were not significant in some years.

Soil strength measured at the end of the rice-growing season in 1980 and 1981 was significantly greater after continuous no-tillage. The decreasing trend in grain and straw yield and number of panicle-bearing tillers/m2 from 1980 onwards after continuous no-tillage, was associated with increasing soil strength. The results suggest that, where percolation losses of water and nutrients are small, puddling, which is capital intensive and detrimental to soil structure, could be replaced by notillage accompanied by suitable N and weed management. However, continuous no-tillage is not recommended for a soil with a lower clay content because the soil will gradually harden with time.

Field studies conducted in 1983/84–1985/86 at Ludhiana showed that the response of physiological and yield characters of Brassica juncea to foliar sprays of chlorocholine chloride as cycocel (CCC) at 250 and 500 p.p.m. and ethrel at 500, 1000 and 1500 p.p.m. differed at different doses of N (O, 50 and 100 kg/ha). The crop did not respond to CCC or ethrel in the absence of N, whereas a significant response was obtained with 250 p.p.m. CCC or 500 p.p.m. ethrel at 50 kg N/ha and 500 p.p.m. CCC or 1000 p.p.m. ethrel at 100 kg N/ha. Response to increasing doses of N increased in the presence of CCC or ethrel spray. The highest concentration of ethrel (1500 p.p.m.) proved detrimental at 0 and N/ha. CCC and ethrel reduced the crop canopy, enhanced the chlorophyll content of leaves, interception of photosynthetically active radiation and sink capacity (number of pods per plant and 1000-seed weight) at 50 and 100 kg N/ha. A higher leaf area index was obtained during the pod development phase with CCC and ethrel sprays. The oil content and germination potential of seeds from crops treated with CCC (250 and 500 p.p.m.) and ethrel (500 and 1000 p.p.m.) were as high as in the untreated crop, irrespective of N dose. However, 1500 p.p.m. of ethrel sprayed on a crop raised without N suppressed germination capacity.

Source–sink balance was studied by imposing different canopy defoliation treatments on wheat crops grown in Los Banos (Philippines) in 1985/86 and 1986/87, Sonora (Mexico) in 1972/73 and 1974/75 and New Delhi (India) in 1987/88. The crops were grown in replicated trials with optimum cultural management. Six defoliation treatments were imposed at anthesis on all shoots in the canopy in an area ranging between 1·65 and 3·0 m2. Defoliation reduced dry weight in proportion to the reduction in percentage light interception. The number of grains per unit land area was reduced slightly, and in most cases not significantly, except when all leaves were removed. Despite reduction of leaf lamina area index to as low as 0·5, the decrease in grain yield was small. In particular, flag leaf removal led to a remarkably small reduction in grain yield. Grain nitrogen content in defoliated crops decreased much less than expected from the amount of N removed by defoliation. The slope of the relation between reduction in grain yield with defoliation and reduction in post-anthesis dry matter accumulation was 0·56, indicating moderate source limitation for grain filling. The crops at the hottest site, in the Philippines, were less limited by source than the other crops. It is suggested that selection for smaller flag leaves may be worthwhile for high-input wheat crops.

Samples of rumen fluid were removed from pairs of sheep on four grass-hay-based diets 7 h after feeding. Micro-organisms were sedimented by centrifugation and the cell-free supernatant was treated with perchloric acid (PCA) to precipitate protein. The remaining fluid was analysed for peptides by several methods to determine how much peptide escaped degradation. Ammonia interfered with analysis by amino group reagents, especially ninhydrin. In this respect, o-phthalaldehyde and trinitrobenzene sulphonic acid were more specific and more useful than ninhydrin. Use of all these reagents showed that significant quantities of amino groups (equivalent to up to 153 mg amino acid N/1 of rumen fluid) were released by hydrolysis of the PCA extract with 6 M-HCI for 24 h. However, fluorescamine analysis indicated that the peptide content of the unhydrolysed PCA extract was < 3 mg N/1. The true amino acid content of different extracts was resolved by chromatographic amino acid analysis: the sum of individual amino acid concentrations in acid-hydrolysed PCA extracts of extracellular rumen fluid ranged from 7·8 to 14·5 mg N/1. Thus most of the free amino N released by hydrolysis of the PCA extract was not from amino acids, and most of the amino acids that were released were originally present in a form that did not react with fluorescamine. Although none of the methods gave a reliable estimate of peptide concentrations, amino acid analysis provided an upper limit. It was therefore concluded that peptide concentrations in extracellular rumen fluid are much lower than indicated by previous ninhydrin estimations, and that little dietary peptide escapes degradation for a prolonged period in the rumen.

Synovex-H®, a combination of testosterone propionate and oestradiol benzoate, was implanted on three occasions at about 90-day intervals into Hereford × Shorthorn (HS), Brahman (B) and BX(B × HS) heifers to try to increase live weights and hence pregnancy rates at first breeding. Half of both the implanted and nonimplanted heifers of each breed was treated every 3 weeks to control cattle ticks and gastro-intestinal helminths. Treatment to control these parasites increased live weights, the increase depending on the breed. Implanting with Synovex-H also increased growth, the magnitude of the increase depending on both breed and treatment to control parasites. When parasites were present in significant numbers in the environment, the implanted groups had higher tick and helminth burdens and, in consequence, their live weight gains in response to the implant were lower than those of the nonimplanted group. The reason for the increase in susceptibility to parasites of the implanted group was not sought. It is suggested that it is associated with the presence, either alone or in combination, of the androgen and oestrogen components of Synovex-H.

By the start of the 10-week breeding season, 290 days after the last dose of Synovex-H was implanted, previous advantages of the implanted groups in live weight had been eroded to the point where the differences between groups were no longer statistically significant. Subsequent fertility both as maidens and as first-calf heifers was generally significantly lower in the previously implanted groups of all breeds. Calves born to implanted heifers were not as heavy as those born to nonimplanted heifers both at weaning and 120 days after weaning. The particular regimen of use of Synovex-H followed in this study cannot be recommended if heifers are to be used for breeding.

Digestibility of neutral detergent fibre (NDF) and monosaccharide components of diets containing 60% untreated straw (UTS) or straw treated with sulphur dioxide (TS) and poultrylitter (1:1) plus 40% concentrate at 700 g/day intake was examined in sheep equipped with rumen and duodenal cannulas. An all-concentrate diet (CD) served as a reference ration. The SO2 treatment of straw increased the apparent digestibility of the NDF, glucose, xylose, arabinose and galactose components of the diet from 58·9, 86·7, 55·7, 82·5 and 91·8%, respectively, in the UTS diet to 73·8, 92·6, 77·8, 88·9 and 94·6%, respectively, in the TS diet. Whereas digestion of NDF and glucose in sheep on the TS diet was slightly lower than in those on the CD diet, digestion of xylose, arabinose and galactose was higher. Thus, the digestibility of total monosaccharides in th TS diet was 90·2 % and that of the CD diet only 61% units higher. The SO2 treatment also increased the total rumen volatile fatty acid (VFA) concentration and the proportion of butyric acid in the total VFA compared with the UTS diet.

Rumen ammonia concentration was 7 mg/100 ml lower and nonammonia nitrogen (NAN) flowto the duodenum was 1·3 g/day higher in sheep fed the TS diet compared with the CD diet. The quantity of duodenal N absorbed in the intestine was 10·7 g/day in the TS diet, close to the value of 11·6 g/day found with the CD diet.

The similarity between the TS and CD diets in total monosaccharides digestion and duodenal Nabsorption, confirms the findings of earlier studies that a TS diet is a highly productive ration.

Complementary interaction between the SO2-treated straw and poultry litter components of the TS diet is discussed.