Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-24T23:02:00.806Z Has data issue: false hasContentIssue false
  • English
  • Français

The influence of time, temperature, pH and calcium carbonate on the activity of the phytase of certain cereals

Published online by Cambridge University Press:  27 March 2009

R. Hill  and
C. Tyler
R. Hill
Affiliation:
Royal Agricultural College, Cirencester*
C. Tyler
Affiliation:
Department of Agricultural Chemistry, The University, Reading
Get access Rights & Permissions [Opens in a new window]

Extract

1. Tests have been carried out to investigate the hydrolysis of phytate in wheat, bran, and oats.

2. Oats showed practically no phytase activity.

3. Wheat phytase had an optimum pH of 5·0–5·1 and functioned at pH values down to 3·0. When hydrolysis had been arrested by acidifying to pH 2·5 for 5 min. or longer, the phytase did not recover when the pH was raised to 5·0 by the addition of sodium bicarbonate.

4. There was an almost uniform increase in the rate of hydrolysis between 15 and 50° C.

5. The rate of phytate hydrolysis decreased as the reaction proceeded, but the reaction continued until only a very small amount of soluble substrate remained.

6. The presence of calcium, under conditions in which it could form insoluble phytate, reduced phytate hydrolysis to a considerable degree.

7. These reactions are considered in relation to phytate hydrolysis in the digestive tracts of animals.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 44 , Issue 3 , June 1954 , pp. 306 - 310
Copyright
Copyright © Cambridge University Press 1954

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, R. J. (1915). J. Biol. Chem. 20, 483.CrossRefGoogle Scholar
Common, R. H. (1940). J. Agric. Sci. 30, 113.CrossRefGoogle Scholar
Cruickshank, E. W. H., Duckworth, J., Kosterlitz, H. W. & Warnock, J. M. (1945). J. Physiol. 104, 41.CrossRefGoogle Scholar
Dukes, H. H. (1947). The Physiology of Domestic Animals, pp. 283 and 330. New York: Comstock Publishing Co.Google Scholar
Giri, K. V. (1938). Chem. Abstr. 32, 9105.Google Scholar
Hassan, K. (1947). Factors affecting the utilisation of phosphorus in the form of phytic acid by ruminants. Thesis. University of Aberdeen.Google Scholar
Hill, R. & Tyler, C. (1954). J. Agric. Sci., 44, 293.CrossRefGoogle Scholar
Hoff-Jørgensen, E. (1947). Nature, Lond., 159, 99.CrossRefGoogle Scholar
Kolobkova, E. V. (1937). Chem. Abstr. 31, 7449.Google Scholar
Krieger, C. H., Bunkfeldt, R. & Steenbock, H. (1940 a). J. Nutr. 20, 7.CrossRefGoogle Scholar
Krieger, C. H., Bunkfeldt, R. & Steenbock, H. (1940 b). J. Nutr. 20, 15.CrossRefGoogle Scholar
Lowe, J. T. & Steenbock, H. (1936). Biochem. J. 30, 1126.CrossRefGoogle Scholar
McCance, R. A. & Widdowson, E. M. (1935). Biochem. J. 29, 2694.CrossRefGoogle Scholar
McCance, R. A. & Widdowson, E. M. (1944). Nature, Lond., 153, 650.CrossRefGoogle Scholar
McGinnis, J., Norris, L. C. & Heuser, G. F. (1944). Poult. Sci. 23, 157.CrossRefGoogle Scholar
Mellanby, E. (1925). Spec. Rep. Ser. Med. Res. Coun., Lond., no. 93.Google Scholar
Mellanby, E. (1949). J. Physiol. 109, 488.CrossRefGoogle Scholar
Mellanby, E. (1950). A Story of Nutritional Research, pp. 224–431. Baltimore: The Williams and Wilkins Co.Google Scholar
Møllgaard, H. (1946). Biochem. J. 40, 589.CrossRefGoogle Scholar
Monroe, C. P. & Perkins, A. E. (1939). J. Dairy Sci. 22, 983.CrossRefGoogle Scholar
Moore, J. H. (1952). The absorption and excretion of minerals by pigs. Thesis, University of Reading.Google Scholar
Patwardhan, V. N. (1937). Biochem. J. 31, 560.CrossRefGoogle Scholar
Pedersen, J. G. A. (1940). 193de Beretning fra Forsøgslaboratoriet. København. (English summary.)Google Scholar
Peers, F. G. (1953). Biochem. J. 53, 102.CrossRefGoogle Scholar
Phillipson, A. T. (1942). J. Exp. Biol. 19, 186.CrossRefGoogle Scholar
Plimmer, R. H. A. (1913). Biochem. J. 7, 43.CrossRefGoogle Scholar
Pringle, W. J. S. & Moran, T. (1942). J. Soc. Chem. Ind., Lond., 61, 108.CrossRefGoogle Scholar
Reid, R. L., Franklin, M. C. & Hallsworth, E. G. (1947). Austr. Vet. J. 23, 136.CrossRefGoogle Scholar
Schulerud, A. (1944). Acta physiol. scand. 8, 259.CrossRefGoogle Scholar
Singsen, E. P. & Mitchell, H. H. (1945). Poult. Sci. 24, 479.CrossRefGoogle Scholar
Spitzer, R. R. & Phillips, R. H. (1945). J. Nutr. 30, 183.CrossRefGoogle Scholar
Starkenstein, E. (1910). Biochem. Z. 30, 56.Google Scholar
Tyler, C. (1946). J. Agric. Sci. 36, 275.CrossRefGoogle Scholar