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Influence of dietary lysine content on wool follicle function in pre-ruminant lambs

Published online by Cambridge University Press:  27 March 2009

R. E. Chapman ,
W. F. Colebrook  and
J. L. Black
R. E. Chapman
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
W. F. Colebrook
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
J. L. Black
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
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Summary

Wool follicles and fibres of pre-ruminant lambs abomasally fed isoenergetic diets with daily amounts of lysine ranging from 0·12 to 1·94 g/(kg live weight)0·75 were examined by light and electron microscopy. In lambs which received ca. 0·9 g lysine/kg 0·76 daily, the follicles appeared normal, whereas in those which received less than 0·9 g lysine/kg0·75 daily several follicle abnormalities developed and the percentages of follicles affected increased as the amount of lysine was decreased. These abnormalities included the formation of autophagic vacuoles (or apoptotic bodies) in follicle bulb cells, and retarded and incomplete keratinization of the wool fibres, which led to the kinking of fibres at the mid-dermal level and gross distortion and partial degradation of fibres in the distal parts of follicles with thickened outer root sheaths. Following the introduction of a low-lysine diet, autophagic vacuoles developed in most follicle bulbs within 24 h. Impaired keratinization and kinking of fibres were evident in increasing proportions of follicles after 2 days, and gross distortion of fibres after 3–4 days. When the lambs were returned to a diet with adequate lysine recovery of normal follicle structure followed a similar time pattern, except that gross distortion of fibres persisted in the distal parts of some follicles for longer than 1 week. In some lambs given more than 0·9 g lysine/kg0·75 daily a small percentage of follicles had short keratogenous zones. Clumping of fibres, which occurred in the proximal parts of a small number of follicles, was unrelated to the amount of lysine in the diet.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 101 , Issue 1 , August 1983 , pp. 139 - 145
Copyright
Copyright © Cambridge University Press 1983

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References

Austin, S. A. & Clemens, M. J. (1981). The regulation of protein synthesis in mammalian cells by amino acid supply. Bioscience Reports 1, 35–44.CrossRefGoogle ScholarPubMed
Barrnett, R. J. & Seligman, A. M. (1952). Histochemical demonstration of protein-bound sulphydryl groups. Science 116, 323–327.CrossRefGoogle Scholar
Bennett, H. S. & Luft, J. H. (1959). 8-Collidine as a basis for buffering fixatives. Journal of Biophysical and Biochemical Cytology 6, 113–114.CrossRefGoogle ScholarPubMed
Chapman, R. E. (1971). Cell migration in wool follicles of sheep. Journal of Cell Science 9, 791–803.CrossRefGoogle ScholarPubMed
Chapman, R. E. (1980). A comparison of the effects of some defleecing compounds on wool follicles, fibres and skin of sheep. In Wool Harvesting Research and Development. Proceedings of the first Conference on Wool Harvesting Research and Development, Melbourne, May 14–16, 1979, pp. 271–286 (ed. Hudson, P. R. W.). Melbourne: Australian Wool Corporation.Google Scholar
Chapman, R. E. (1981). Autophagy – a means of cell removal in wool follicles. Journal of Anatomy 132, 304.Google Scholar
Chapman, R. E. & Black, J. L. (1981). Abnormal wool growth and alopecia of artificially reared lambs. Australian Journal of Biological Sciences 34, 11–26.CrossRefGoogle Scholar
Chapman, R. E. & Reis, P. J. (1978). Effects of abomasal supplements of methionine on the wool follicles and skin of wheat-fed sheep. Australian Journal of Biological Sciences 31, 161–172.CrossRefGoogle ScholarPubMed
Chapman, R. E. & Wheeler, J. L. (1963). Dye-banding: a technique for fleece growth studies. Australian Journal of Science 26, 53–54.Google Scholar
Colebrook, W. F. & Black, J. L. (1978). Comparison of methods for estimating the lysine requirement of lambs. Proceedings of the Nutrition Society of Australia 3, 73.Google Scholar
Colebrook, W. F. & Black, J. L. (1981). Influence of dietary lysine content on energy utilization in preruminant lambs. Animal Production 33, 253–258.Google Scholar
Downes, A. M. & Sharry, L. F. (1971). Measurement of wool growth and its response to nutritional changes. Australian Journal of Biological Sciences 24, 117–130.CrossRefGoogle ScholarPubMed
Gemmell, R. T. & Chapman, R. E. (1971). Formation and breakdown of the inner root sheath and features of the pilary canal epithelium of the wool follicle. Journal of Ultrastructure Research 36, 355–356.CrossRefGoogle ScholarPubMed
Kerr, J. F. R., Wyllie, A. H. & Currie, A. R. (1972). Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. British Journal dermato of Cancer 26, 239–257.CrossRefGoogle ScholarPubMed
Mortimore, G. E. (1982). Mechanisms of cellular protein catabolism. Nutrition Reviews 40, 1–12.CrossRefGoogle ScholarPubMed
Mortimore, G. E. & Schworer, C. M. (1977). Induction of autophagy by amino-acid deprivation in perfused rat liver. Nature, London 270, 174–176.CrossRefGoogle ScholarPubMed
Raphael, K. A., Chapman, R. E., Frith, P. A. & Pennycuik, P. R. (1982). The structure of hair and follicles of mice carrying the naked (N) gene. Genetical Research, Cambridge 39, 139–148.CrossRefGoogle ScholarPubMed
Reis, P. J. & Colebrook, W. F. (1972). The utilization of abomasal supplements of proteins and amino acids by sheep with special reference to wool growth, Australian Journal of Biological Sciences 25, 1057–1071.CrossRefGoogle ScholarPubMed
Reis, P. J. & Downes, A. M. (1971). The rate of response of wool growth to abomasal supplements of casein. Journal of Agricultural Science, Cambridge 76, 173–176.CrossRefGoogle Scholar
Reis, P. J. & Tunks, D. A. (1976). The influence of abomasal supplements of zein and some amino acids on wool growth rate and plasma amino acids. Journal of Agricultural Science, Cambridge 86, 475–482.CrossRefGoogle Scholar
Sanderson, C. J. (1976). The mechanism of T cell mediated cytotoxicity. II. Morphological studies of cell death by time-lapse microcinematography. Proceedings of the Royal Society of London, Series B, 192, 241–255.Google ScholarPubMed
Weedon, D., Searle, J. & Kerr, J. F. R. (1979). Apoptosis. Its nature and implications for dermatoof pathology. The American Journal of Dermatopathology 1, 133–144.CrossRefGoogle Scholar