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Influence of dietary lysine content on wool follicle function in pre-ruminant lambs

Published online by Cambridge University Press:  27 March 2009

R. E. Chapman ,
W. F. Colebrook  and
J. L. Black
R. E. Chapman
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
W. F. Colebrook
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
J. L. Black
Affiliation:
Division of Animal Production, C.S.I.R.O., PO Box 239, Blacktoum, 2148 New South Wales, Australia
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Summary

Wool follicles and fibres of pre-ruminant lambs abomasally fed isoenergetic diets with daily amounts of lysine ranging from 0·12 to 1·94 g/(kg live weight)0·75 were examined by light and electron microscopy. In lambs which received ca. 0·9 g lysine/kg 0·76 daily, the follicles appeared normal, whereas in those which received less than 0·9 g lysine/kg0·75 daily several follicle abnormalities developed and the percentages of follicles affected increased as the amount of lysine was decreased. These abnormalities included the formation of autophagic vacuoles (or apoptotic bodies) in follicle bulb cells, and retarded and incomplete keratinization of the wool fibres, which led to the kinking of fibres at the mid-dermal level and gross distortion and partial degradation of fibres in the distal parts of follicles with thickened outer root sheaths. Following the introduction of a low-lysine diet, autophagic vacuoles developed in most follicle bulbs within 24 h. Impaired keratinization and kinking of fibres were evident in increasing proportions of follicles after 2 days, and gross distortion of fibres after 3–4 days. When the lambs were returned to a diet with adequate lysine recovery of normal follicle structure followed a similar time pattern, except that gross distortion of fibres persisted in the distal parts of some follicles for longer than 1 week. In some lambs given more than 0·9 g lysine/kg0·75 daily a small percentage of follicles had short keratogenous zones. Clumping of fibres, which occurred in the proximal parts of a small number of follicles, was unrelated to the amount of lysine in the diet.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 101 , Issue 1 , August 1983 , pp. 139 - 145
Copyright
Copyright © Cambridge University Press 1983

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