Sheep were grazed for 2 years at stocking rates of 7, 14, 21 and 28/ha on a pasture comprising Lotononis bainesii and Digitaria decumbens cv. Pangola at Mt Cotton, south–east Queensland. There were six replicates of each treatment grazed in rotation with 3 days' grazing followed by 15 days' rest.

The initial dominance of lotononis was lost after 6 months of grazing and lotononis failed to persist satisfactorily at any stocking rate. Demographic studies showed that lotononis behaved as a short-lived plant, predominantly annual, with some vegetative perennation as stolon-rooted units under heavy grazing. Soil seed reserves varied from 5800 to 400 m2 at the lightest and heaviest stocking rates respectively. Lotononis failed to regenerate under Pangola shading or inopportune high grazing pressure. Soil bulk density (0–7 cm) increased from 1·2 to 1·4 g/cm3 according to stocking rate.

A pasture of Lotononis bainesii and Digitaria decumbens cv. Pangola at Mt Cotton, south-east Queensland was mown on six occasions at intervals of ca. 3 months and after each mowing was subjected in 1 day to the treading intensity expected from sheep grazing at 0, 7, 14, 21 and 28/ha. A movable corridor and folds were employed to walk the sheep the required number of times on eight replicates of each treatment.

Lotononis regrowth was negatively related to sheep treading intensity, whereas Pangola was highly resistant to treading and grew vigorously from underground buds on rhizomes and from persisting stolons. Young lotononis seedlings were more vulnerable than older seedlings which had branched; plants developed from autonomous nodal roots were more resistant than seedlings. Attention is drawn to the contractile growth of the hypocotyl following epigeal germination which leads to a buried crown.

Lotononis plants were short-lived, and seedling regeneration of ca. 27 and 47 plants/m2 occurred in the late summer of the 2nd and 3rd year after sowing. Soil seed reserves averaged 16900/m2. Studies might be undertaken of management systems directed to favouring lotononis plant replacement through accretion to seed reserves, seedling regeneration through the creation of ‘gaps’, and seedling survival through judicious timing of grazing.

Studies were conducted to elucidate the nature and cause of the drastic losses of live weight of cattle grazing buffel grass pastures after first rains at the end of the dry season in northern Australia. This paper examines trends in weight and body composition during the dry and early wet seasons; it shows that although most of the loss in fasted live weight occurred just after first rain, most of the loss of body solids, mainly fat, had already occurred by this time. Losses of body solids in the dry season were not fully reflected in live-weight losses because of increases in total body water and in gut ‘fill’. The greatly accelerated weight loss in the period following first rain appears to have been due mainly to a large reduction in gut contents. Empty-body weight actually increased during this period owing to increases in tissue water. Continuation of this trend in tissue water into the early wet season resulted in the rate of live-weight gain greatly exceeding that of body solids.

Cattle grazing buffel grass pastures during the dry season at Katherine lose large amounts of live weight during a short period following first rain. In Part 1 we showed that these losses were due mainly to reduction in gut contents. Part 2 examines the changes in pasture, diet and grazing behaviour to assess the probable change in nutritional status of cattle following rain.

During the dry season the most valuable pasture component, green leaf, was very scarce and had a nitrogen concentration of about 1%. Diets of oesophageally-fistulated cattle contained less than 0·5% N. Shortly after first rain diet N doubled owing both to rapidly increasing amounts of green leaf and to the increase in N concentration in young leaf to over 3%. Although intake was not measured, literature is cited to show that this increase in dietary N would be expected to increase rumen digestion rates with a resultant marked increase in D.M. intake and a reduction in gut contents.

Although availability of high quality herbage increased very rapidly following first rain, a period of increased nutritional stress immediately following rain could not be ruled out. However, any such decline in nutrition appears to be short-lived.

An emulsion of [3H]triglycerides was used to investigate how circulating triglycerides are partitioned between milk fat, body fat and use as an energy source in lactating sheep. Immediately after intravenous injection of the emulsion the radioactivity in the blood was predominantly associated with the triglycerides of the fraction that contained chylomicrons and very low density lipoproteins. The radioactive triglyceride was used both as a precursor of milk fat and as a source of energy. The proportions utilized for these two purposes were determined experimentally and the amount retained in the carcass lipids was found by difference. In separate control experiments with nonlactating sheep the total radioactivity in the body lipids was determined by carcass analysis. This total together with that accounted for by oxidation amounted to 98% of the total radioactivity injected. These results established that the emulsion was a reliable model for the purpose intended.

The proportion of the labelled triglyceride that appeared in milk fat declined from 45% at 17 days after lambing to 8–10% at 73 days after lambing. The proportion that was oxidized was also lower in late lactation so that retention in carcass lipids was substantially increased at that time.

Hypocuprosis has been diagnosed for 4 years in calves of a beef suckler herd at Warren Farm, Berkshire. The copper (Cu) contents and distribution in the soil at that site, as well as changes in herbage Cu throughout the 1979 grazing season, were examined. The herbage Cu concentration was always less than 7·5 μg/g and decreased markedly to 3·0 μg/g during July. Serum Cu contents of both untreated cows and untreated calves also decreased markedly, but at a much earlier stage than the decrease in herbage concentration. One untreated calf developed severe anaemia, but recovered when treated with Cu Ca EDTA. Although changes in the coefficient of absorption of dietary Cu were caused by changes in S and Mo contents, the calculated availability of Cu was dominated by the Cu content per se. Thus the calculated availability remained relatively high during the period when serum concentration decreased.

The decrease in serum Cu may have occurred as the result of a differing availability of Cu in ensiled and grazed herbages. However, coincident with the decrease in serum Cu was a high concentration of Fe in the grazed herbage which was largely associated with the surfaces of the leaves. It is therefore possible that the development of Cu deficiency immediately after the animals started to graze was precipitated either through an interaction between Cu and Fe in the animal, or because of a reduced availability of Cu through an interaction with ingested soil.

A total of 21 rumen-cannulated wether sheep and six rumen-cannulated Ayrshire cows were used in a series of experiments to investigate fermentation in the rumen in animals given silage diets and the nominal metabolism of silage lactic acid. Dietary experiments with sheep (three experiments) and cows (two experiments) given diets consisting of formic acid–preserved perennial ryegrass (Lolium perenne) silages, in some cases with supplements of rolled barley, were conducted to investigate the ruminal concentrations of lactic acid and of volatile fatty acids (VFA) after feeding. Ingestion of silage was followed by a short-lived peak in lactic acid concentration in the rumen and by a subsequent increase in the concentration of propionate or propionate and butyrate. Butyrate production was more evident in cattle than in sheep. Taken as an average over a 6 h period of sampling the proportions (m-mol/mol total VFA) of acetate, propionate and butyrate in the rumen in animals given silage alone were typically 590–600, 210–250 and 70–150 respectively. When silage was supplemented with barley, in both sheep and cattle, the proportion of propionate was reduced and the proportion of butyrate was increased. In one experiment with sheep these changes in fermentation pattern were shown to be accompanied by an increase in the number of rumen protozoa from 2·78×105/ml to 18·66·105/ml (P <0·01). However, in a subsequent experiment it was shown that defaunation led to an increase in the proportion of butyrate in the rumen.

Twelve estimates were also made with sheep of the metabolism of lactic acid infused into the rumen in a single dose of 20–50 g. These experiments showed that both (L+) and DL-lactio acid were metabolized equally rapidly with a half-life of approximately 25 min. In faunated animals the major products of fermentation were propionate with a small amount of butyrate. In defaunated animals lactate metabolism was slowed and the major product of fermentation was butyrate.

In vitro studies of lactate fermentation were carried out using strained liquor and isolated bacterial and protozoal fractions from the rumen of sheep receiving silage diets. The rates of lactate disappearance (μg/mg protein/h) were 50 and 22·2 for the rumen liquor and bacterial fraction, respectively, which were significantly (P <0·05, n = 5) less than the rate of 294·7 for the protozoal fraction. Rumen liquor, bacterial and protozoal fractions yielded a similar mixture of fermentation end-products, propionate accounting for approximately 60% of the VFA produced.

The results are discussed in relation to the metabolism of silage lactic acid in the rumen, the production of VFA and the yield of ATP for microbial cell synthesis. It is concluded that the pathways of lactate fermentation are regulated by the rumen microbial population and that protozoa may have an important role in the regulation of lactate fermentation.

Generalized lattice designs (a member of the class of incomplete block designs) have been used in 13 potato variety yield trials over 3 years. Results from these designs are shown to be generally more precise than from comparable complete block designs.

Field and pot investigations of the vegetative and early reproductive growth of a winter wheat and a winter rye cultivar over three seasons (1978–9, 1980–2) have shown that higher and earlier forage yields of rye are due to a combination of factors, notably more rapid rates of germination, crop emergence, leaf appearance and leaf expansion coupled with higher leaf area ratios. There was no evidence, from studies of net assimilation rate, that the photosynthetic apparatus of rye plants is better adapted than wheat to the low temperature and light conditions of winter and early spring. However, the earlier initiation of rye stem extension was associated with significant increases in net assimilation rate, which compensated for reductions in the leaf area ratio. Practical aspects of these results are discussed briefly.

Methods of controlling Sitona lineatus adults and larvae were tested in 13 experiments on spring-sown beans, four on autumn-sown beans and two on dry-harvested peas. A total of 16 insecticides were used, though not all combinations of insecticide, application method and crop were tested. Applications included granules or sprays to the foliage, seed-bed treatments, seed-furrow treatments and seed treatments. Phorate as a furrow treatment at sowing at 1·24 kg a.i./ha increased yields of spring-sown beans by an average of 0·5 t/ha but was not persistent enough to protect autumn-sown beans from attack. Foliar applications of systemic insecticides (carbofuran or carbosulfan) at similar doses decreased attacks but effects on yield need further evaluation. Preliminary work indicates that seed-bed or furrow treatments of phorate can also be used to control S. lineatus on peas.

Five experiments which studied the effects of a wide range of dates of planting on contrasting potato varieties in Pembrokeshire are reported. In three experiments (1976–7) four early varieties (Home Guard, Arran Comet, Irish Peace and Ulster Sceptre) were sprouted from the end of dormancy and compared at four dates of planting, which began as soon as soil conditions allowed (February in 1975 and 1976 and March in 1977). In these experiments all early-emerging treatments were damaged by frost and in 1975 and 1976 date of planting had little effect on leaf area index or yield. In 1977 planting in late April delayed and increased peak leaf area index but reduced yields throughout harvesting. In all experiments the emergence of varieties was affected by date of planting. The varieties with the longest sprouts emerged first only from the earliest plantings; at late plantings all varieties emerged together, which suggests that rate of post-planting sprout elongation decreased in this old seed as planting was delayed despite increasing soil temperatures. The implications for testing of early varieties are discussed.

In two further experiments two early varieties (Home Guard in both years and Red Craigs Royal and Arran Comet in 1 year) were compared with three maincrop varieties (Désirée, Maris Piper, Stormont Enterprise) using seed which did not begin to sprout until January at dates of planting beginning in March. Sprout length was again poorly related to earliness of emergence. Delaying planting delayed and increased peak leaf area index in all varieties but only increased yields in the early varieties which had the smallest leaf areas. In maincrop varieties date of planting had little effect on final yields. In these years there were long periods without rain and in 1976 yields were limited by the amount of water available from the soil, for as each treatment exhausted this supply bulking ceased.

Five Romney wethers, fitted with rumen fistulae, were each fed five pelleted diets high in starch (30–50%) containing 0·72, 1·22, 1·72, 2·47 and 3·72% N respectively. Samples of rumen liquor were incubated with glycerol-tri-[1-14C]oleate or with [1-14C]- linoleic acid. The rates of lipolysis or hydrogenation of these substrates were measured. In addition rumen contents of the sheep when fed the 1·22% N diet were incubated with radioactive substrates plus 0, 100 and 200 mg finely ground casein.

It was found that there was an approximately linear increase in the rate of lipolysis between 0·7 and 2·5% N. The rate of hydrogenation did not increase below l·2% N and tended to decrease above 2·5% N. The potential for hydrogenation of unesterified linoleic acid was calculated to be 4–6 times greater with all diets except that containing 1·2% N. This may be one explanation for the elevated concentration of the rumen unsaturated fatty acids in sheep fed diets containing ca. 1–1·5% N.

It was also established that the addition of finely ground casein to the incubate was without effect.

It was concluded that microbial composition is important in regulating the relative rates of lipolysis and hydrogenation which determined the amount of unsaturated dietary fatty acid present in the rumen.

Results are presented from two experiments (1973 and 1974) in which high potato yields were obtained (60–70 t/ha). In both, Désirée and Maris Piper accumulated much more N and K (> 200 and 300 kg/ha respectively) than was applied as fertilizer (160 kg N and 189 kg K/ha). Increasing planting density (to levels above commercial practice) increased nutrient accumulation but had only small effects on yields. Thus, high yields are associated with but not necessarily caused by large accumulations of nutrients. As a general principle, increasing fertilizer rates above those shown to be optimal will not increase yields.

Three varieties of triticale (TL-238, TL-257 and TL-319) bred by the Plant Breeding Department of Punjab Agricultural University were studied for nutrient composition and metabolizable energy (ME) content for egg-type chicks. A feeding trial was also conducted to study the suitability of triticale as a replacement for maize in conventional high- and low-cereal diets for different classes of egg-type chickens.

The crude protein contents of TL-238, TL-257 and TL-319 were respectively 13·4,14·4 and 2·1%. Corresponding values for starch content were 51·5, 49·2 and 54·3%, respectively. ME in triticale varieties closely followed the trend of starch content. Accordingly TL-319 was higher in ME (13·58 MJ/kg D.M.) than TL-238 and TL-257 (12·91 and 12·49 MJ/kg D.M. respectively). There were no significant differences in body-weight gain or in food conversion of chicks fed diets containing different varieties of triticale. The substitution of triticale for maize in a conventional high-cereal diet improved growth and food conversion of starter chicks. In a low-cereal diet, however, triticale had an adverse effect on the growth rate and food conversion of starter chicks. At the end of 18 weeks of age, the differences in body-weight gain, food conversion and efficiency of protein utilization by chicks on all the treatments were not significantly different (P > 0·05). Results of the production phase showed that egg production, egg mass, food conversion and energetic efficiency were significantly (P < 0·05) improved by feeding triticale.

Two sources of sulphur-coated urea (SCU) were compared with calcium ammonium nitrate (CAN) as sources of N for cotton and sorghum in the savannah zone of Nigeria. At the lowest rate of N application (30 kg/ha for cotton and 40 kg/ha for sorghum), SCU-11 with a dissolution rate of 11% in 7 days was found to be more effective than SCU-30 having a dissolution rate of 30% in 7 days and CAN. A single application of SCU-11 produced a higher sorghum yield than a divided application of CAN at the same rate. These results indicate that a slow-release nitrogen fertilizer might be useful for improved grain sorghum and cotton varieties.

The residual effects of sulphur-coated urea (SCU) fertilizers were evaluated by measuring the pH and mineral-N in the soil profile after 2 years of application of these fertilizers to soils in the various ecological zones of the savannah of Nigeria. Changes of 0·2–0·3 pH units relative to the control (without applied N) were detected on the sandier soils at Kadawa and Mokwa but not on the heavier Samaru soils. There was little or no difference among the sulphur-coated urea and calcium ammonium nitrate in their acidification effects. Also residual nitrogen levels were low in plots that received SCU but did not differ significantly from those of calcium ammonium nitrate.

Simulating the accumulation of dead leaf sheaths in perennial ryegrass by masking the tiller bases increased leaf and sheath length and tiller weight but decreased tiller production. Exposure of previously masked tiller bases or stripping back the subtending leaf and sheath to expose the tiller bud in its axil stimulated tiller production. A photomorphogenic effect is postulated which controls the partitioning of dry matter between the growth of new leaf and sheath tissue on existing tillers, and the development of new tillers from tiller buds.

Wool follicles and fibres of pre-ruminant lambs abomasally fed isoenergetic diets with daily amounts of lysine ranging from 0·12 to 1·94 g/(kg live weight)0·75 were examined by light and electron microscopy. In lambs which received ca. 0·9 g lysine/kg 0·76 daily, the follicles appeared normal, whereas in those which received less than 0·9 g lysine/kg0·75 daily several follicle abnormalities developed and the percentages of follicles affected increased as the amount of lysine was decreased. These abnormalities included the formation of autophagic vacuoles (or apoptotic bodies) in follicle bulb cells, and retarded and incomplete keratinization of the wool fibres, which led to the kinking of fibres at the mid-dermal level and gross distortion and partial degradation of fibres in the distal parts of follicles with thickened outer root sheaths. Following the introduction of a low-lysine diet, autophagic vacuoles developed in most follicle bulbs within 24 h. Impaired keratinization and kinking of fibres were evident in increasing proportions of follicles after 2 days, and gross distortion of fibres after 3–4 days. When the lambs were returned to a diet with adequate lysine recovery of normal follicle structure followed a similar time pattern, except that gross distortion of fibres persisted in the distal parts of some follicles for longer than 1 week. In some lambs given more than 0·9 g lysine/kg0·75 daily a small percentage of follicles had short keratogenous zones. Clumping of fibres, which occurred in the proximal parts of a small number of follicles, was unrelated to the amount of lysine in the diet.

This study examined the effect of early sowing of sugar beet on plant establishment, bolting and yield and whether or not the standard of establishment and degree of bolting determined the most appropriate harvesting sequence. At Arthur Rickwood Experimental Husbandry Farm four experiments were made between 1974 and 1978 testing four sowing dates of four varieties each harvested on three occasions during the autumn. At Broom's Barn three dates of sowing of two varieties were tested in three experiments between 1976 and 1978. Sowing dates throughout the experiments covered a range from late February to late April. Also, at Broom's Barn in 1977 there were many bolters (plants which have a tall, flower-bearing stem) where the crop was sown on 4 March. The effects of bolter-control treatments, either cutting down the inflorescence or removing the plant, on sugar yield were compared. Sowing before mid-March often resulted in very gappy crops and many bolters; in consequence yields were seriously reduced. Late-March sowings were almost free from plant establishment problems and, where the bolting-resistant variety ‘Nomo’ was used, bolters were few; yields of Nomo were greater than where sown in early April. Progressively more yield was lost by delaying sowing throughout April. Variation in the extent of bolting between sites, seasons and sowing-date treatments was accounted for by variation in the number of cool days (with a maximum temperature of less than 12 °C) after sowing. Yield was lost at a rate of 0·7% for every 1% of bolters over the range 5–40% bolting. During the autumn, crops at Arthur Rickwood which had more than 5% bolters and/or less than 60000 plants/ha gained 0·45 t/ha less sugar than did those with full, bolterfree plant stands. In 1977 many of the early-sown plants bolted; pulling the bolters or cutting the inflorescences improved yield, but not sufficiently to raise yields to the level produced by later-sown, bolter-free crops.

Opium poppy (Papaver somniferum Linn.) was sown at three sowing dates (10, 25 November, 10 December), row spacings (15, 30, 45 cm) and population densities (6 × 105, 3 × 105, 1·5 × 105 plants/ha) in a split-plot design at Lucknow. Sowing on 10 November resulted in higher yields of opium, seed, total dry matter and morphine content. Delayed sowing, higher plant density and narrow spacing produced low yields with less morphine content. Decline in opium yield was associated with decrease in plant height, leaf area index, capsule size and number of capsules per plant. The crop sown on 10 November removed most N. N concentration and uptake also increased when the number of plants per unit area was decreased. The study suggests that to achieve maximum yields poppy should be sown in rows 30 cm apart on 10 November with 3 × 105 plants/ha.