The future biosphere on Earth (as with its past) will be made up predominantly of unicellular micro-organisms. Unicellular life was probably present for at least 2.5 Gyr before multicellular life appeared and will likely be the only form of life capable of surviving on the planet in the far future, when the ageing Sun causes environmental conditions to become more hostile to more complex forms of life. Therefore, it is statistically more likely that habitable Earth-like exoplanets we discover will be at a stage in their habitable lifetime more conducive to supporting unicellular, rather than multicellular life. The end stage of habitability on Earth is the focus of this work. A simple, latitude-based climate model incorporating eccentricity and obliquity variations is used as a guide to the temperature evolution of the Earth over the next 3 Gyr. This allows inferences to be made about potential refuges for life, particularly in mountains and cold-trap (ice) caves and what forms of life could live in these environments. Results suggest that in high latitude regions, unicellular life could persist for up to 2.8 Gyr from present. This begins to answer the question of how the habitability of Earth will evolve at local scales alongside the Sun's main sequence evolution and, by extension, how the habitability of Earth-like planets would evolve over time with their own host stars.

With the aim to visualize the span of time since the formation of our Universe we have set up a nature and hiking trail called ‘Time Trek’. The 13.7 km length of the trail corresponds to the age of the Universe, and portrays its history including events important for Earth and life. One kilometre corresponds to a billion years, and one metre to a million years of time. The trek combines astronomical, physical, geological and biological time lines, and presents a holistic view of the history of time. It helps people to comprehend the causal and temporal connections of different phenomena. To the trekker, it offers a concrete experience of the lengths and proportions of different time periods, which otherwise are very difficult to understand.

Previous work in studying interstellar exploration by one or several probes has focused primarily either on engineering models for a spacecraft targeting a single star system, or large-scale simulations to ascertain the time required for a civilization to completely explore the Milky Way Galaxy. In this paper, a simulated annealing algorithm is used to numerically model the exploration of the local interstellar neighbourhood (i.e. of the order of ten parsecs of the Solar System) by a fixed number of probes launched from the Solar System; these simulations use the observed masses, positions and spectral classes of targeted stars. Each probe visits a pre-determined list of target systems, maintains a constant cruise speed, and only changes the direction from gravitational deflection at each target. From these simulations, it is examined how varying design choices – differing the maximum cruise speed, number of probes launched, number of target stars to be explored, and probability of avoiding catastrophic system failure per parsec – change the completion time of the exploration programme and the expected number of stars successfully visited. In addition, it is shown that improving this success probability per parsec has diminishing returns beyond a certain point. Future improvements to the model and possible implications are discussed.

If confirmed, the extremely low concentrations of methane (CH4) detected in the Martian atmosphere may represent reservoirs and emission processes that would normally be considered negligible on Earth. One such process is the release of ancient volatiles from fluid inclusions and interstitial sites in rocks and minerals during erosion or geothermal activity. Using a highly sensitive rock-crushing and mass-spectrometry technique previously shown to detect CH4 in serpentinites and hydrothermal mineral deposits, we have demonstrated that CH4 and other ancient volatiles can be recovered from basalt, the dominant rock type on the Martian surface. Basalt samples from a wide range of ages and geological systems were tested, all of which released CH4 when crushed. Oxidative weathering was associated with lower quantities of CH4. Otherwise, CH4 recoverability showed no relationship with age or geological context. Mineral veins, cross-cutting one locality were found to share the volatile composition of the basalt. In general, the results suggest that CH4-release from ancient basalts could be a significant process on Mars, which could be further investigated by Martian rovers using a similar rock-crushing and mass spectrometry technique in situ.

Although astrobiology is a science midway between the life and physical sciences, it has surprisingly remained largely disconnected from recent trends in certain branches of both life and physical sciences. We discuss potential applications to astrobiology of approaches that aim at integrating rather than reducing. Aiming at discovering how systems properties emerge has proved valuable in chemistry and in biology. The systems approach should also yield insights into astrobiology, especially concerning the ongoing search for alternative abodes for life. This is feasible since new data banks in the case of astrobiology – considered as a branch of biology – are of a geophysical/astronomical kind, rather than the molecular biology data that are used for questions related firstly, to genetics in a systems context and secondly, to biochemistry for solving fundamental problems, such as protein or proteome folding. By focusing on how systems properties emerge in astrobiology we consider the question: can life in the universe be interpreted as an emergent phenomenon? In the search for potential habitable worlds in our galactic sector with current space missions, extensive data banks of geophysical parameters of exoplanets are rapidly emerging. We suggest that it is timely to consider life in the universe as an emergent phenomenon that can be approached with methods beyond the science of chemical evolution – the backbone of previous research in questions related to the origin of life. The application of systems biology to incorporate the emergence of life in the universe is illustrated with a diagram for the familiar case of our own planetary system, where three Earth-like planets are within the habitable zone (HZ) of a G2 V (the complete terminology for the Sun in the Morgan–Keenan system) star. We underline the advantage of plotting the age of Earth-like planets against large atmospheric fraction of a biogenic gas, whenever such anomalous atmospheres are discovered in these worlds. A prediction is made as to the nature of the atmospheres of the planets that lie in the stellar HZs.

Titan, the largest satellite of Saturn, has a thick atmosphere containing nitrogen and methane. A variety of organic compounds have been detected in the atmosphere, most likely produced when atmospheric gases are exposed to ultraviolet light, electrons captured by the magnetosphere of Saturn and cosmic rays. The Cassini/Huygens probe showed that the average temperature on the surface of Titan is 93.7 K, with lakes of liquid ethane and methane. Sub-surface mixtures of liquid ammonia and water may also be present. We have synthesized complex organic compounds (tholins) by exposing a mixture of nitrogen and methane to plasma discharges, and investigated their interactions with several different liquids that simulate Titan's liquidosphere. We found that coacervates formed when tholins were extracted in non-polar solvents followed by exposure to aqueous ammonia solutions. The results suggest that coacervates can self-assemble in Titan's liquidosphere which have the potential to undergo further chemical evolution. Similar processes are likely to occur in the early evolution of habitable planets when tholin-like compounds undergo phase separation into microscopic structures dispersed in a suitable aqueous environment.

Bioleaching has been suggested as an alternative to traditional mining techniques in extraterrestrial environments because it does not require extensive infrastructure and bulky hardware. In situ bioleaching of silicate minerals, such as those found on the moon or Mars, has been proposed as a feasible alternative to traditional extraction techniques that require either extreme heat and/or substantial chemical treatment. In this study, we investigated the biotic and abiotic leaching of basaltic rocks (analogues to those found on the moon and Mars) and the mineral ilmenite (FeTiO3) in aqueous environments under acidic (pH ∼ 2.5) and circumneutral pH conditions. The biological leaching experiments were conducted using Acidithiobacillus ferrooxidans, an iron (Fe)-oxidizing bacteria, and Pseudomonas mendocina, an Fe-scavenging bacteria. We found that both strains were able to grow using the Fe(II) derived from the tested basaltic rocks and ilmenite. Although silica leaching rates were the same or slightly less in the bacterial systems with A. ferrooxidans than in the abiotic control systems, the extent of Fe, Al and Ti released (and re-precipitated in new solid phases) was actually greater in the biotic systems. This is likely because the Fe(II) leached from the basalt was immediately oxidized by A. ferrooxidans, and precipitated into Fe(III) phases which causes a change in the equilibrium of the system, i.e. Le Chatelier's principle. Iron(II) in the abiotic experiment was allowed to build up in solution which led to a decrease in its overall release rate. For example, the percentage of Fe, Al and Ti leached (dissolved + reactive mineral precipitates) from the Mars simulant in the A. ferrooxidans experimental system was 34, 41 and 13% of the total Fe, Al and Ti in the basalt, respectively, while the abiotic experimental system released totals of only 11, 25 and 2%. There was, however, no measurable difference in the amounts of Fe and Ti released from ilmenite in the experiments with A. ferrooxidans versus the abiotic controls. P. mendocina scavenged some Fe from the rock/mineral substrates, but the overall amount of leaching was small (<2% of total Fe in rocks) when compared with the acidophilic systems. Although the mineralogy of the tested basaltic rocks was roughly similar, the surface areas of the lunar and Mars simulants varied greatly and thus were possible factors in the overall amount of metals released. Overall, our results indicate that the presence of bacteria does not increase the overall silica leaching rates of basaltic rocks; however, the presence of A. ferrooxidans does lead to enhanced release of Fe, Al and Ti and subsequent sequestration of Fe (and other metals) in Fe(III)-precipitates.

In the past 15 years, astronomers have revealed that a significant fraction of the stars should harbour planets and that it is likely that terrestrial planets are abundant in our galaxy. Among these planets, how many are habitable, i.e. suitable for life and its evolution? These questions have been discussed for years and we are slowly making progress. Liquid water remains the key criterion for habitability. It can exist in the interior of a variety of planetary bodies, but it is usually assumed that liquid water at the surface interacting with rocks and light is necessary for emergence of a life able to modify its environment and evolve. The first key issue is thus to understand the climatic conditions allowing surface liquid water assuming a suitable atmosphere. These have been studied with global mean one-dimensional (1D) models which have defined the ‘classical habitable zone’, the range of orbital distances within which worlds can maintain liquid water on their surfaces (Kasting et al. 1993). A new generation of 3D climate models based on universal equations and tested on bodies in the solar system are now available to explore with accuracy climate regimes that could locally allow liquid water. The second key issue is now to better understand the processes which control the composition and the evolution of the atmospheres of exoplanets, and in particular the geophysical feedbacks that seem to be necessary to maintain a continuously habitable climate. From that point of view, it is not impossible that the Earth's case may be special and uncommon.

Cyanobacteria are capable of surviving the adverse conditions of low Earth orbit (LEO). We have previously demonstrated that Gloeocapsa strain OU_20, Chroococcidiopsis and akinetes of Anabaena cylindrica were able to survive 548 days of exposure to LEO. Motivated by an interest to understand how cyanobacteria can survive in LEO, we studied the strategies that Gloeocapsa strain OU_20 employs to survive in its natural environment, the upper region of the intertidal zone. Here, cyanobacteria are exposed to fluctuations in temperature, UV radiation, desiccation and salinity. We demonstrated that an increase in salinity from 6.5‰ (BG-11 medium) to 35.7‰ (similar to that of seawater), resulted in increased resistance to UV radiation (254 nm), vacuum (0.7×10−3±0.01 kPa) and cold temperatures (–20 °C). Concomitantly, biochemical analyses demonstrated that the amount of fatty acids and mycosporine-like amino acids (a UV absorbing pigment) were higher in the stressed cells. Morphological analysis demonstrated that the electron density and thickness of the mucilaginous sheath were also greater than in the control cells. Yet, the control and stressed cells both formed aggregates. As a result of studying the physiological adaptation of Gloeocapsa strain OU_20 in response to salinity, we postulate that survival in the high-intertidal zone and LEO involves a dense extracellular mucilaginous sheath and the formation of aggregates. We conclude that studying the physiological adaptation of cyanobacteria in the intertidal zone provides insight into understanding survival in LEO.

We consider the suggestion that, as a matter of caution, the deliberate broadcast of signals to the cosmos should be proscribed. We explore the likely capabilities of extraterrestrial societies that might conceivably pose a threat to our species and show that this suggestion is without merit because even if followed, it would fail to keep our existence secret. In addition, trying to limit signalling activities would be a burden on our descendants, crippling their own activities for the indefinite future. A corollary to these considerations affects the search for extraterrestrial intelligence (SETI): namely, it seems inarguable that technologically advanced beings will assume that other societies are capable of detecting their leakage. Consequently, the premise that SETI should expect to find deliberate beacons from other worlds may be mistaken.

Subglacial ecosystems have recently become of interest within the astrobiological community, as they represent a potentially habitable location in otherwise uninhabitable environments. We used data from Blood Falls, particularly the periodic discharge from the subglacial reservoir beneath Taylor Glacier, Antarctica, to construct an ecosystem model of the putative subglacial microbial community residing there using system dynamics modelling. The model results were, for the most part, within an order of magnitude of the geochemical field data. Productivity was quite low, at 6.4×10−5 g carbon l−1 yr−1. Based on the results, we draw the following conjectures for the search for life on Mars: A similar ecosystem would require a continual supply of oxidized iron for energy and generate significant amounts of reduced iron as a waste product, be relatively resilient to temporary disturbances, and, thermodynamically, would require at least 0.003 kJ mol l−1 of energy to survive at that level of productivity. These results may help to better identify the constraints and boundaries of ecosystems in extreme environments, on Earth and other planetary bodies.

The paper considers the two-century-old problem of how solar spots influence biological objects on the Earth. It describes the modern state of the kT-problem, which for a long time has been the most difficult obstacle in explaining solar activity effects. Based on recent advances in spin chemistry and magnetoplasticity physics, it is shown that a ‘molecular target’ sensitive to weak electromagnetic fields is spins in non-equilibrial states of the molecular system. A way of how solar spots can influence Earth's molecular, including biological, processes through a ‘transparency window’ in the Earth's atmosphere is proposed.

The fI term of Drake's equation – the fraction of life-bearing planets on which ‘intelligent’ life evolved – has been the subject of much debate in the last few decades. Several leading evolutionary biologists have endorsed the thesis that the probability of intelligent life elsewhere in the universe is vanishingly small. A discussion of this thesis is proposed here that focuses on a key issue in the debate: the existence of evolutionary trends, often presented as trends towards higher complexity, and their possible significance. The present state of knowledge on trends is reviewed. Measurements of quantitative variables that describe important features of the evolution of living organisms – their hierarchical organization, size and biodiversity – and of brains – their overall size, the number and size of their components – in relation to their cognitive abilities, provide reliable evidence of the reality and generality of evolutionary trends. Properties of trends are inferred and frequent misinterpretations (including an excessive stress on mere ‘complexity’) that prevent the objective assessment of trends are considered. Finally, several arguments against the repeatability of evolution to intelligence are discussed. It is concluded that no compelling argument exists for an exceedingly small probability fI. More research is needed before this wide-ranging negative conclusion is accepted.

In the present work, the interactions between forsterite-91 with distilled water and forsterite-91 with artificial seawater were studied at two pHs (2.0 and 8.0) using different techniques. A large increase in pH was observed for samples incubated at an initially acidic pH (2.0) due to the dissolution of forsterite-91 in distilled water and artificial seawater. Thus, in acidic hydrothermal vents, an increase in the amount of hydrocarbons and magnetite should be expected due to the release of Fe(II). The pHPZC decreased and the pHIEP increased when forsterite-91 was treated with distilled water and artificial seawater. The ions from the artificial seawater had an effect on zeta potential. Scanning electron microscopy (SEM) images and X-ray diffractograms showed halite in the samples of forsterite-91 mixed with artificial seawater. The presence of halite or adsorption of ions on the surface of forsterite-91 could affect the synthesis of magnetite and hydrocarbons in hydrothermal vents, due to a decrease in the dissolution rates of forsterite-91. The dissolution of forsterite-91 yields low concentrations of Fe(III) and Mn(II) as detected by electron paramagnetic resonance (EPR) spectroscopy. Microanalysis of forsterite-91 showed a higher amount of Mn, with an oxidation that was likely not +II, as Mn in supernatant solutions was only detected by EPR spectroscopy after mixing with artificial seawater at pH 2.0. As Fe(III) and Mn(II) are catalyst constituents of magnetite and manganese oxide, respectively, their presence is important for synthesis in hydrothermal vents. Etch pits were observed only in the forsterite-91 sample mixed with distilled water at pH 8.0. Na, Cl, S, Ca and K were detected in the samples mixed with artificial seawater by SEM–EDS. Si, Mg, Fe and Al were detected in almost all supernatant samples due to forsterite-91 dissolution. Cr was not dissolved in the experiments, thus Cr in the mineral could serve as an effective catalyst for Fischer Tropsch Types (FTT) reactions in hydrothermal vent systems. X-ray diffractograms of the original forsterite-91 also showed peaks arising from zeolites and clinochlore. After the samples were treated with artificial seawater, X-ray diffractograms showed the dissolution of zeolite. Experiments should be performed in the natural environment to verify the potential for zeolites to act as a catalyst in hydrothermal vents.

Gale crater formed from an impact on Mars ∼3.6 billion years ago. It hosts a central mound nearly 100 km wide and ∼5 km high, consisting of layered rocks with a variety of textures and spectral properties. The oldest exposed layers contain variably hydrated sulphates and smectite clay minerals, implying an aqueous origin, whereas the younger layers higher on the mound are covered by a mantle of dust. Fluvial channels carved into the crater walls and the lower mound indicate that surface liquids were present during and after deposition of the mound material. Numerous hypotheses have been advocated for the origin of some or all minerals and layers in the mound, ranging from deep lakes to playas to mostly dry dune fields to airfall dust or ash subjected to only minor alteration driven by snowmelt. The complexity of the mound suggests that multiple depositional and diagenetic processes are represented in the materials exposed today. Beginning in August 2012, the Mars Science Laboratory rover Curiosity will explore Gale crater by ascending the mound's northwestern flank, providing unprecedented new detail on the evolution of environmental conditions and habitability over many millions of years during which the mound strata accumulated.

This paper discusses ideas that impact the fc factor as defined by Frank Drake in 1961, i.e. the fraction of planets with intelligent creatures capable of interstellar communication. This factor remains one of the most speculative terms of the equation. We suggest that the ability of sharing information is an important parameter to take into account in evaluating the tendency of a civilization to make contact (or share data) with other civilizations. Thus, we give special consideration to the fraction of planets with intelligent creatures capable of producing and sharing large amount of data. First, we determine the level of our own civilization in the framework of Sagan's energy- and information-based classification, by taking into account the recent improvements in computing and networking technologies. Second, we distinguish two types of organization, hierarchical and heterarchical, with respect to information sharing. We illustrate this distinction in the case of SETI and we show that the probability to detect a civilization would be greater if it is heterarchical than if it is hierarchical and if we utilize heterarchical principles for SETI.

We proffer a contemporary solution to the so-called Fermi Paradox, which is concerned with conflict between Copernicanism and the apparent paucity of evidence for intelligent alien civilizations. In particular, we argue that every community of organisms that reaches its space-faring age will (1) almost immediately use its rocket-building computers to reverse-engineer its genetic chemistry and (2) self-destruct when some individual uses said technology to design an omnicidal pathogen. We discuss some of the possible approaches to prevention with regard to Homo sapiens’ vulnerability to bioterrorism, particularly on a short-term basis.

Fermi's paradox is founded on the idea that one or more Galactic extraterrestrial civilizations (ETCs) existed long ago and sustained exploration for millions of years, but in spite of their advanced knowledge, they could not find a way to explore the Galaxy other than with fleets of starships or self replicating probes. Here, I question this second assumption: if advanced technology generally allows long-distance remote sensing, and if ETCs were motivated by gaining information rather than conquest or commerce, then such voyages would be unnecessary, thereby resolving Fermi's paradox.