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The genetic basis of response in mouse lines divergently selected for body weight or fat content. II. The contribution of genes with a large effect

Published online by Cambridge University Press:  14 April 2009

Roel F. Veerkamp ,
Chris S. Haley ,
Sara A. Knott  and
Ian M. Hastings
Roel F. Veerkamp*
Affiliation:
Genetics and Behaviourial Sciences Department, Scottish Agricultural College, West Mains Road, Edinburgh, EH9 3JG, Scotland AFRC Roslin Institute (Edinburgh), Roslin, EH25 9PS, Scotland
Chris S. Haley
Affiliation:
AFRC Roslin Institute (Edinburgh), Roslin, EH25 9PS, Scotland
Sara A. Knott
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland
Ian M. Hastings
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland
*
* Corresponding author
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Gene action underlying selection responses has been studied using crossbreeding. Maximum likelihood based segregation analysis has been presented for analysing backcross data for the presence of genes with a large effect. Two sets of divergently selected lines (P-lines for body weight and F-lines for fat content) were reciprocally crossed and the F1s were crossed to the high and low lines to produce all possible backcrosses. Earlier analysis had shown that the difference in body weight at 10 weeks (n = 595) between the high and low P-lines was largely (75–80%) explained by autosomal, additive genes with the remainder explained by additive genes on the X chromosome. Maximum likelihood segregation analysis suggested the presence of a major effect on the X chromosome, but as there was only one round of recombination between the X chromosomes in the forming of the backcrosses, linked genes on the X chromosome could have acted together to give the appearance of a single major gene. The difference in fat content between the F-lines (n = 578) could be explained by autosomal genes of largely additive effect. Segregation analysis suggested the presence of a major gene with complete dominance, but this was attributed to a relationship between the mean and the variance: transformation of the data resulted in only polygenic additive genes being of importance. This study concluded that maximum likelihood based analysis and crosses between selected lines provide a powerful means for studying the gene action underlying responses to selection.

Type
Research Article
Information
Genetics Research , Volume 62 , Issue 3 , December 1993 , pp. 177 - 182
Copyright
Copyright © Cambridge University Press 1993

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