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GERMINATION OF UNTREATED AND PRIMED SEEDS IN RAPESEED (BRASSICA NAPUS VAR OLEIFERA DEL.) UNDER SALINITY AND LOW MATRIC POTENTIAL

Published online by Cambridge University Press:  06 January 2012

ROBERTA PACE ,
PAOLO BENINCASA ,
MICHEL EDMOND GHANEM ,
MURIEL QUINET  and
STANLEY LUTTS
ROBERTA PACE*
Affiliation:
Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74 – 06121 Perugia, Italy
PAOLO BENINCASA
Affiliation:
Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74 – 06121 Perugia, Italy
MICHEL EDMOND GHANEM
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
MURIEL QUINET
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
STANLEY LUTTS
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
*
‡Corresponding author. Email: [email protected]
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Summary

Salt (NaCl) and matric stress (polyethylene glycol; PEG 6000) impacts on germination were analysed in two rapeseed (Brassica napus var oleifera Del.) cultivars (Exagone: salt and water stress tolerant; Toccata: salt and water stress sensitive), which had the same imbibition kinetics when incubated in distilled water. Final germination percentage (G) and time to reach 50% G (T50) were affected either by 325 mM NaCl or −0.9 MPa PEG in Toccata but not in Exagone. Cultivars did not differ for protein concentration and protease activity in any germination condition; thus, protein metabolism seems not to be implicated in response to stress. Hydroxurea and cytochalasine-D inhibited germination in Toccata exposed to −0.9 MPa PEG but not in Exagone, suggesting that Exagone tolerance could be due to a lower amount of damaged DNA and lower requirement for DNA replication due to cell elongation rather than cell division for radicle elongation. Soluble sugars concentration increased in salt-stressed seeds from Exagone more than in Toccata and this could have allowed osmotic adjustment and therefore cell elongation despite the presence of external NaCl. Priming treatments with gibberellic acid, kinetin, NaCl and PEG hastened seed germination under control conditions but had no effect or even negative effects on germination under both salt and matric stresses.

Type
Research Article
Information
Experimental Agriculture , Volume 48 , Issue 2 , April 2012 , pp. 238 - 251
Copyright
Copyright © Cambridge University Press 2012

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