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A study of enterotoxigenic Escherichia coli, serogroup 0126, by bacterial restriction endonuclease DNA analysis (BRENDA)

Published online by Cambridge University Press:  19 October 2009

R. B. Marshall ,
P. J. Winter ,
A. J. Robinson  and
K. A. Bettelheim
R. B. Marshall
Affiliation:
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand
P. J. Winter
Affiliation:
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand
A. J. Robinson
Affiliation:
M.R.C. Virus Research Unit, University of Otago, P.O. Box 56, Dunedin, New Zealand
K. A. Bettelheim
Affiliation:
National Health Institute, Porirua, New Zealand
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Summary

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Sixteen isolates of Escherichia coli were subjected to bacterial restriction endonuclease DNA analysis (BRENDA). Nine of these isolates were from an outbreak of human diarrhoea and produced stable toxin, the remaining seven were non-toxigenic strains from animal and human sources. The isolates from the outbreak produced indistinguishable DNA electrophoretic patterns in spite of their assignment to seven different H serotypes. Their BRENDA patterns were markedly different from the other isolates examined. These results support the epidemiological evidence that a single-strain outbreak had occurred, and they cast doubt on the value of H typing for this particular investigation.

Type
Research Article
Information
Journal of Hygiene , Volume 94 , Issue 3 , June 1985 , pp. 263 - 268
Copyright
Copyright © Cambridge University Press 1985

References

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