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A diagnostic strategy to determine the Shiga toxin-producing Escherichia coli O157 status of pens of feedlot cattle

Published online by Cambridge University Press:  26 February 2004

D. R. SMITH ,
J. T. GRAY ,
R. A. MOXLEY ,
S. M. YOUNTS-DAHL ,
M. P. BLACKFORD ,
S. HINKLEY ,
L. L. HUNGERFORD ,
C. T. MILTON  and
T. J. KLOPFENSTEIN
D. R. SMITH
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA
J. T. GRAY
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA Present address: USDA, ARS, Antimicrobial Resistance Research Unit, Athens, GA, USA.
R. A. MOXLEY
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA
S. M. YOUNTS-DAHL
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA Present address: Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, USA.
M. P. BLACKFORD
Affiliation:
Department of Animal Science, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA
S. HINKLEY
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA
L. L. HUNGERFORD
Affiliation:
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA Present address: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
C. T. MILTON
Affiliation:
Department of Animal Science, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA Present address: 10249 W. Loup River Road, Dannebrog, NE, USA.
T. J. KLOPFENSTEIN
Affiliation:
Department of Animal Science, Institute of Agriculture and Natural Resources, University of Nebraska–Lincoln, USA
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Abstract

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Although cattle are reservoirs, no validated method exists to monitor Shiga toxin-producing Escherichia coli O157 (STEC O157) on farms. In 29 Midwestern United States feedlot pens we compared culturing faeces from the individual cattle to: (1) culturing rope devices that cattle rub or chew; and (2) culturing a composite of faecal pats. Eighty-six per cent (68–96%) of pens were classified correctly using rope devices to detect pens with at least 16% of the cattle shedding STEC O157 [sensitivity=82% (57–96%); specificity=92% (62–100%)]. Ninety per cent of pens (73–98%) were classified correctly using composite faeces to detect pens with at least 37% of the cattle shedding STEC O157 [sensitivity=86% (42–100%); specificity=91% (71–99%)]. Ranking pens into three risk levels based on parallel interpretation of the pen-test results correlated (Spearman's r=0·76, P<0·0001) with the pen's prevalence. This strategy could identify pens of cattle posing a higher risk to food safety.

Type
Research Article
Information
Epidemiology & Infection , Volume 132 , Issue 2 , April 2004 , pp. 297 - 302
Copyright
© 2003 Cambridge University Press