We tested for plague (Yersinia pestis) in a puma population in the Greater Yellowstone Ecosystem (GYE) over 9 years, overlapping a case when a boy in the area became infected with plague. Antibodies to Y. pestis were detected in 8 of 17 (47%) pumas tested by complement-enzyme-linked immunosorbent assay, and the organism itself was detected in 4 of 11 (36%) pumas tested after necropsy. Neither puma sex nor age was significantly associated with Y. pestis exposure or mortality, although our sample size was small. The overall prevalence of exposure we recorded was similar to that found along the western slope of Colorado, which is adjacent to the Four Corners region, a known plague hotspot in the USA. This suggests that: (1) Y. pestis may be present at higher levels in the GYE than previously assumed; (2) plague is a significant source of mortality for local pumas (6.6% of sub-adult and adult mortality); and (3) pumas may be a useful sentinel for potential risk of plague exposure to humans throughout the West. We would also emphasize that hunters and others handling pumas in this region should be made aware of the possibility of exposure.

The Ecuadorian coast has two main types of forests, which are differentiated by their phenology: dry forests are deciduous and more humid forests are evergreen. Less of the dry forests on the Ecuadorian coast is protected (13% of its area) than the evergreen forests (28%), and the area devoted to the protection of dry forests (1069 km2) is substantially less than the area devoted to the protection of evergreen forests (2800 km2). Yet the conservation status of dry forests is more critical, with 83% of their area classified as having very low connectivity, 70% as being highly fragile and 86% as being highly threatened. In addition, the dry forests have fewer protected areas than the evergreen forests. It is therefore necessary to increase the protection of deciduous ecosystems as part of a comprehensive national strategy because they support high levels of biodiversity and many endemic species.

Attempts to mitigate the environmental impact of oil-palm expansion by improving production standards have been of limited success. An alternative approach aims at a better understanding of the factors that drive consumers to reduce excessive vegetable oil consumption. Our studies focused on consumers’ environmental knowledge as a potential determinant of palm oil consumption. They revealed critical limitations in consumers’ palm oil-related knowledge across different domains, study settings and assessment approaches. The more our participants knew about palm oil and its environmental consequences, the stronger were their reported intentions to reduce their consumption. This relationship was significantly stronger for subjective versus objective palm oil-related knowledge. In addition, we examined whether consumers can be stimulated to consult information about palm oil by making information access the default option. While this simple situational nudge promoted information access, we did not find it to significantly increase actual engagement with the information material. This result suggests that it might be necessary to complement default nudges for information access with other measures to enhance palm oil-related knowledge and to reduce palm oil consumption to more sustainable levels.

Creating landscapes with connectivity is vital for protecting biodiversity and meeting the environmental targets embedded in the United Nations Sustainable Development Goals, with connectivity specifically mentioned in Target 11 of the Convention on Biological Diversity Aichi Targets. Costa Rica created the National Biological Corridor Program (NBCP) in 2006 to enhance connectivity among protected areas. Targeted investments of payments for environmental services (PES) are the main tools used within the designated biological corridors. We conducted spatially explicit analyses to determine whether Costa Rica’s NBCP, using PES, enhanced landscape connectivity within the Paso de las Nubes Biological Corridor. We conducted landscape modelling in order to determine the connectivity held within PES’s properties by developing connectivity resistance surfaces and electrical current models. The results indicate that PES properties established after the NBCP contributed more to areas with intermediate values of connectivity and less to areas with high connectivity values as compared to properties before the NBCP. Although overall connectivity within the corridor has decreased since NBCP establishment, our results confirm the importance of PES properties for landscape connectivity, but emphasize the need for spatially targeted PES in order to improve viable paths of landscape connectivity among protected areas. Future targeted PES investments could contribute greatly to meeting connectivity goals.

Soybean has undergone the greatest expansion of any global crop, fuelled by the emergence of herbicide-resistant crops. In Uruguay, soybean croplands have increased from virtually zero to more than 1 million ha in 20 years. Uruguay is also implementing its system of protected areas. Here, we assess the presence of pesticides within a Ramsar site and protected area, in a basin dominated by croplands. We consider pesticides as surrogates of the subtle impacts of agribusiness on conservation initiatives and other productions. Pesticides were found in soils, fishes and beehives, both within and around the protected area. Endosulfan was found in all matrices analysed (23 of 80 samples), while glyphosate (0–2.31 mg/kg) and aminomethylphosphonic acid (AMPA; 0–0.61 mg/kg) were found in all soil classes. The study also allowed for a retrospective evaluation of a recent policy banning endosulfan in Uruguay, suggesting that while the protected area has not been immune to the impacts of agribusiness on human health or biodiversity, limiting the use of pesticides reduces or avoids some of them. This has implications for the design of multifunctional landscapes and for the debate on land sharing versus land sparing.

The scope and scale of wildlife crimes around the world have risen in intensity and complexity, yet current enforcement strategies have often not delivered desired effects on illegal activities, even within protected areas. Tackling the array of illegal activities by emphasizing law enforcement above other options is challenging and potentially unsustainable. We explored the potential for social norms, community regulations and socioeconomic factors to promote compliance with wildlife laws by interviewing 334 respondents in 28 villages that share boundaries with protected areas in Nigeria. Using an anonymous direct questioning approach, we recorded a high prevalence of non-compliance behaviours in all studied communities. Injunctive norms (i.e., perceptions of acceptable behaviour within a social group) significantly predicted compliance, as respondents with no complicit friends or family members were more likely to comply with wildlife regulations. Perceived likelihood of community-level sanctions played a more salient role than the fear of arrest by rangers in influencing compliance. In addition, non-compliance increased with number of dependents, but reduced with average monthly household income. Our study demonstrates that clear knowledge of the social norms that drive local behaviour as well as the authorities that enforce them is integral to understanding the forces that drive community involvement and participation in conservation. Incorporating local communities in planning enforcement interventions may help protect threatened species and landscapes.

Despite their legal protection status, protected areas (PAs) can benefit from priority ranks when ongoing threats to their biodiversity and habitats outpace the financial resources available for their conservation. It is essential to develop methods to prioritize PAs that are not computationally demanding in order to suit stakeholders in developing countries where technical and financial resources are limited. We used expert knowledge-derived biodiversity measures to generate individual and aggregate priority ranks of 98 mostly terrestrial PAs on Madagascar. The five variables used were state of knowledge (SoK), forest loss, forest loss acceleration, PA size and relative species diversity, estimated by using standardized residuals from negative binomial models of SoK regressed onto species diversity. We compared our aggregate ranks generated using unweighted averages and principal component analysis (PCA) applied to each individual variable with those generated via Markov chain (MC) and PageRank algorithms. SoK significantly affected the measure of species diversity and highlighted areas where more research effort was needed. The unweighted- and PCA-derived ranks were strongly correlated, as were the MC and PageRank ranks. However, the former two were weakly correlated with the latter two. We recommend using these methods simultaneously in order to provide decision-makers with the flexibility to prioritize those PAs in need of additional research and conservation efforts.

Many of Europe’s unique habitats are highly threatened. In order to tackle these threats, the European Union (EU) is annually financing, mainly through its LIFE programme, conservation actions aimed at improving the conservation status of its habitats. We analysed the allocation of the programme’s budget since its inception in 1992 and we found that the number of projects implemented within the EU member states is only weakly related to the number of habitats within them (R2 = 0.39). In some states, fewer than 25% of the habitats have been funded, while in others, more than 75% of the habitats have been funded. There are also disparities in terms of which habitats are being funded; a quarter of them have never received any funding, while others have been targeted by multiple projects. Transnational cooperation between the states is low, further perpetuating the aforementioned disparities. Projects are implemented almost exclusively within the recipient state, often irrespectively of the conservation status of the targeted habitats in other states. We recommend that the EU addresses these disparities by encouraging projects in underfunded states, especially in habitats with unfavourable conservation statuses. Moreover, the EU should encourage transnational cooperation in order to promote effective conservation across the EU and to help underfunded states build their capacity.

Decisions made by grassland owners are crucial for the conservation of extensive grasslands (EGs); however, the predispositions determining how adults decide to act in certain situations are developed during childhood. Children are future decision-makers and potential grassland owners; therefore, the factors that form and affect their attitudes towards EG conservation need to be examined. Positive attitudes could be developed during environmental education, and this study aimed to understand the influence of elementary school children’s interest in and knowledge of grasslands, sources of learning about grasslands and experience working with grasslands on their attitudes towards preserving EGs. Principal component analysis and structural equation modelling results showed that interest in grasslands directly influenced attitudes towards preserving EGs, while experience and learning also indirectly influenced these attitudes. Children’s interest should be stimulated through gathering experience with grasslands and education in order to resolve any potential misconceptions about EG management.