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PCR–RFLP identification of endemic Chilean species of Rhagoletis (Diptera: Tephritidae) attacking Solanaceae

Published online by Cambridge University Press:  09 March 2007

M. Salazar ,
C. Theoduloz ,
A. Vega ,
F. Poblete ,
E. González ,
R. Badilla  and
L. Meza-Basso
M. Salazar
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
C. Theoduloz
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
A. Vega
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
F. Poblete
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
E. González
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
R. Badilla
Affiliation:
Servicio Agrícola y Ganadero, VII Región, TalcaChile
L. Meza-Basso*
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
*
*Fax: 56 71 200279 E-mail: [email protected].
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Abstract

Four species of the genus Rhagoletis are native to Chile: R. nova (Schiner), R. conversa, (Brèthes), R. penela Foote and R. tomatis Foote. Currently, identification of these species is based on morphological criteria, but their strong similarity makes precise recognition difficult. To clarify species separation for quarantine purposes, a reliable method based on a PCR–RFLP procedure is reported. A DNA region containing mitochondrial NADH dehydrogenase genes was selected as a target sequence for the analysis. The amplification products (c. 1 kb) were digested with either SspI or DdeI, yielding specific patterns that differentiated each of the endemic species. Complete nucleotide sequences were determined, confirming empirical restriction maps. This report updates information on the geographical distribution of Rhagoletis species in Chile.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 92 , Issue 4 , August 2002 , pp. 337 - 341
Copyright
Copyright © Cambridge University Press 2002

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