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NADPH-cytochrome P450 reductase involved in the lambda-cyhalothrin susceptibility on the green mirid bug Apolygus lucorum

Published online by Cambridge University Press:  02 October 2024

Congai Zhen Open the ORCID record for Congai Zhen [Opens in a new window] ,
Rui Wu ,
Yao Tan Open the ORCID record for Yao Tan [Opens in a new window] ,
Ansheng Zhang  and
Lei Zhang Open the ORCID record for Lei Zhang [Opens in a new window]
Congai Zhen
Affiliation:
Department of Entomology, China Agricultural University, Beijing 100193, P.R. of China
Rui Wu
Affiliation:
Department of Entomology, China Agricultural University, Beijing 100193, P.R. of China
Yao Tan*
Affiliation:
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China
Ansheng Zhang
Affiliation:
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Plant Virology, Jinan 250100, China
Lei Zhang*
Affiliation:
Department of Entomology, China Agricultural University, Beijing 100193, P.R. of China
*
Corresponding authors: Lei Zhang; Email: [email protected]; Yao Tan; Email: [email protected]
Corresponding authors: Lei Zhang; Email: [email protected]; Yao Tan; Email: [email protected]
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Abstract

NADPH-cytochrome P450 reductase (CPR) is crucial for the detoxification process catalysed by cytochrome P450, which targets various exogenous xenobiotics, as well as pesticides. In our research, we successfully obtained the complete cDNA sequence of Apolygus lucorum's CPR (AlCPR) using reverse transcription PCR along with rapid amplification of cDNA ends technology. Bioinformatics analysis exhibited that the inferred amino acid sequence of AlCPR is characteristic of standard CPRs, featuring an N-terminal membrane anchor and three conserved FMN, FAD and NADP binding sites. Phylogenetic result revealed that AlCPR was positioned within the Hemiptera cluster, showing a close evolutionary relationship with the CPR of Cimex lectularius. The real-time quantitative PCR results demonstrated widespread expression of AlCPR across various life stages and tissues of A. lucorum, with the most prominent expression in adults and the abdominal region. Injecting double-stranded RNA of AlCPR only significantly increased the lambda-cyhalothrin susceptibility in lambda-cyhalothrin-resistant strain rather than the susceptible strain. These findings suggest a potential link between AlCPR and the P450-dependent defence mechanism against lambda-cyhalothrin in A. lucorum.

Keywords

Apolygus lucorumlambda-cyhalothrin resistanceNADPH-cytochrome P450 reductase
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 5 , October 2024 , pp. 699 - 706
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

These authors have contributed equally to this work.

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