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The chemical characterization of the epicuticular hydrocarbons of Aedes aegypti (Diptera: Culicidae)

Published online by Cambridge University Press:  09 March 2007

G.L. Horne  and
A.A. Priestman
G.L. Horne
Affiliation:
Department of Biological Sciences, School of Sciences, Staffordshire University, College Road, Stoke-on-Trent, ST4 2DE, UK
A.A. Priestman*
Affiliation:
Department of Biological Sciences, School of Sciences, Staffordshire University, College Road, Stoke-on-Trent, ST4 2DE, UK
*
*Fax: +44 (0) 1782 745506 E-mail: [email protected]
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Abstract

The chemical characterization of the hydrocarbon fraction of the epicuticular lipids of the vector mosquito Aedes aegypti (Linnaeus) was performed using gas chromatography (GC) and gas chromatography–electron impact mass spectrometry (GC–MS). Seventy eight compounds were detected in purified hexane extracts and of these, 42 hydrocarbons were identified and several of the remaining compounds were partially characterized. The hydrocarbon classes present were n-alkanes, monomethylalkanes, dimethylalkanes and alkenes and the results were similar to those published for other Aedes species. Quantitative comparisons of cuticular hydrocarbon profiles were made between males and females, different age groups and between a standard laboratory strain and a recently colonized strain of A. aegypti. These results provide baseline data for further studies on the possible role of mosquito cuticular hydrocarbons in the modification of mating behaviour.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 92 , Issue 4 , August 2002 , pp. 287 - 294
Copyright
Copyright © Cambridge University Press 2002

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References

Blomquist, G.J., Nelson, D.R. & de Renobles, M. (1987) Chemistry, biochemistry and physiology of insect cuticular lipids. Archives of Insect Biochemistry and Physiology 6, 227–265.CrossRefGoogle Scholar
Carlson, D.A. & Schlein, Y. (1991) Unusual polymethyl alkenes in tsetse flies acting as abstinon in in Glossina morsitans. Journal of Chemical Ecology 17, 267–284.CrossRefGoogle Scholar
Carlson, D.A., Mayer, M.S., Silhacek, D.L., James, J.D., Beroza, M. & Bierl, B.A. (1971) Sex attractant pheromone of the housefly: isolation, identification and synthesis. Science 174, 76–77.CrossRefGoogle Scholar
Carlson, D.A., Langley, P.A. & Huyton, P. (1978) Sex pheromone of the tsetse fly: isolation, identification and synthesis of contact aphrodisiacs. Science 201, 750–753.CrossRefGoogle ScholarPubMed
Chen, C.S., Mulla, M.S., March, R.B. & Chaney, J.D. (1990) Cuticular hydrocarbon patterns in Culex quinquefasciatus as influenced by age, sex and geography. Bulletin of the Society of Vector Ecology 15, 129–139.Google Scholar
Desena, M.L., Clark, J.M., Edman, J.D., Symington, S.B., Scott, T.W. & Peters, T.M. (1999) Potential for ageing female Aedes aegypti (Diptera: Culicidae) by gas chromatographic analysis of cuticular hydrocarbons, including a field evaluation. Journal of Medical Entomology 36, 811–823.CrossRefGoogle ScholarPubMed
Horne, G.L. (2000) The biochemistry and biological roles of the epicuticular lipid layer of the mosquito. In Aedes aegypti. (Diptera: Culicidae). PhD thesis, Staffordshire University.Google Scholar
Jackson, L.L. & Blomquist, G.J. (1976) Insect waxes. pp. 201–233. In Kolattukudy, P.E. (Ed.) Chemistry and biochemistry of natural waxes. Amsterdam: Elsevier.Google Scholar
Kruger, E.L. & Pappas, C.D. (1993) Geographic variation of cuticular hydrocarbons among fourteen populations of Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology 30, 544–548.CrossRefGoogle ScholarPubMed
Kruger, E.L., Pappas, C.D. & Howard, R.W. (1991) Cuticular hydrocarbon geographic variation among seven North American populations of Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology 28, 859–864.CrossRefGoogle ScholarPubMed
Lockey, K.H. (1988) Lipids of the insect cuticle: origin, composition and function. Comparative Biochemistry and Physiology 89, 595–645.Google Scholar
Milligan, P.J.M., Phillips, A., Molyneux, D.H., Subbarao, S.K. & White, G.B. (1986) Differentiation of Anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components. Bulletin of Entomological Research 76, 529–537.CrossRefGoogle Scholar
Munstermann, L.E. (1990) Gene map of the yellow fever mosquito (Aedes (Stegomyia) aegypti). pp 3.170–3.183. in O'Brien, S.J. (Ed.), Genetic maps: locus maps of complex genomes. 5th edn. Cold SpringHarbor Laboratory Press.Google Scholar
Nelson, D.R. (1978) Long chain methyl-branched hydrocarbons: occurrence, biosynthesis and function. Advances in Insect Physiology 13, 1–33.CrossRefGoogle Scholar
Oguma, Y., Nemoto, T. & Kuwhara, Y. (1992) (Z)-11-Pentacosene is the major sex pheromone component in Drosophila virilis (Diptera). Chemoecology 3, 60–64.CrossRefGoogle Scholar
Oguma, Y., Nemoto, T. & Kuwhara, Y. (1992) A sex pheromone study of a fruit fly Drosophila virilis Sturtevant (Diptera: Drosophilidae): additive effect of cuticular alkadienes to the major sex pheromone. Applied Entomology and Zoology 27, 499–505.CrossRefGoogle Scholar
Pappas, C.D., Bricker, B.J., Christen, J.A. & Rumbaugh, S.A. (1994) Cuticular hydrocarbons of Aedes hendersoni Cockerell and A. triseriatus (Say). Journal of Chemical Ecology 20, 1121–1136.CrossRefGoogle Scholar
Phillips, A., Sabatini, A., Milligan, P.J.M., Boccolini, D., Broomfield, G. & Molyneux, D.H. (1990) The Anopheles maculipennis complex (Diptera: Culicidae) comparison of the cuticular hydrocarbon profiles determined in adults of five Palaearctic species. Bulletin of Entomological Research 80, 459–464.CrossRefGoogle Scholar
Uebel, E.C., Sonnet, P.E., Bierl, B.A. & Miller, R.W. (1975) Sex pheromone of the stable fly: isolation and preliminary identification of compounds that induce mating strike behaviour. Journal of Chemical Ecology 1, 377–385.CrossRefGoogle Scholar
Uebel, E.C., Sonnet, P.E., Menzer, R.E., Miller, R.W. & Lusby, W.R. (1977) Mating-stimulant pheromone and cuticular lipid constituents of the little house fly, Fannia canicularis (L.). Journal of Chemical Ecology 3, 269–278.CrossRefGoogle Scholar