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Detection of Brugia malayi in laboratory and wild-caught Mansonioides mosquitoes (Diptera: Culicidae) using Hha I PCR assay

Published online by Cambridge University Press:  09 March 2007

S.L. Hoti ,
V. Vasuki ,
M.W. Lizotte ,
K.P. Patra ,
G. Ravi ,
P. Vanamail ,
A. Manonmani ,
S. Sabesan ,
K. Krishnamoorthy  and
S.A. Williams
S.L. Hoti*
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
V. Vasuki
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
M.W. Lizotte
Affiliation:
Clark Science Centre, Smith College, Northampton, USA
K.P. Patra
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
G. Ravi
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
P. Vanamail
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
A. Manonmani
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
S. Sabesan
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
K. Krishnamoorthy
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
S.A. Williams
Affiliation:
Clark Science Centre, Smith College, Northampton, USA
*
*Fax: 0413-372422, 372041 E-mail: [email protected]
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Abstract

An Hha 1 based polymerase chain reaction (PCR) assay developed for the detection of Brugia malayi, the causative agent of Brugian lymphatic filariasis, was evaluated for its sensitivity in the laboratory and for its usefulness in measuring changes in transmission of the disease in the field. Laboratory studies showed that the new assay was highly sensitive in comparison with the standard dissection and microscopy technique. The assay can detect as little as 4 pg of parasite DNA or a single microfilaria in pools of up to 100 mosquitoes. The optimum pool size for convenience was found to be 50 mosquitoes per pool. The efficacy of PCR assay was evaluated in filariasis control programmes in operation in endemic areas of Kerala State, South India. The infection rates obtained by the Hha I PCR assay and the conventional dissection and microscopy technique were 1.2% and 1.7% respectively in operational areas and 8.3% and 4.4% respectively, in check areas, which were not significantly different (P < 0.05). Thus, the Hha I PCR assay was found to be as sensitive as the conventional technique and hence it can be used as a new epidemiological tool for assessing parasite infection in field-collected mosquitoes.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 91 , Issue 2 , April 2001 , pp. 87 - 92
Copyright
Copyright © Cambridge University Press 2001

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