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A western-style diet reduces bone mass and biomechanical bone strength to a greater extent in male compared with female rats during development

Published online by Cambridge University Press:  09 March 2007

Wendy E. Ward ,
Susie Kim  and
W. Robert Bruce
Wendy E. Ward*
Affiliation:
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 3E2
Susie Kim
Affiliation:
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 3E2
W. Robert Bruce
Affiliation:
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 3E2
*
*Corresponding author:Dr Wendy E. Ward, fax +1 416 978 5882, email [email protected]
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Abstract

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Evidence from epidemiological and animal-feeding trials suggests that a western-style diet that is high in fat, and low in Ca, vitamin D and folic acid may result in low bone mass and poor bone quality: this leads to an increased risk of fragility fracture. The overall objective of the present study was to determine the effect of feeding a western-style diet (low in Ca (0·4 g/kg diet, Ca:P ratio 1:10), cholecalciferol (3 μg/kg diet), folic acid (0·23 mg/kg diet) and fibre (20 g/kg diet), and high in fat (200 g/kg diet)) for 17 weeks on bone mineral content (BMC) and the biomechanical bone strength of rat femurs. A secondary objective was to determine whether femurs from male and female rats (seven to eight rats per group) respond differently to the western-style diet. Male and female rats weighing 150–180 g were fed a western-style diet or a control diet for 17 weeks. At the end of the feeding trial, femur BMC was measured by ashing, and biomechanical properties were determined by three-point bending. Femur BMC and the majority of biomechanical properties measured were lower (P<0·05) among male and female rats fed a western-style diet compared with a control diet, despite similar weight gain and final body weight within genders. However, the western-style diet had a greater negative effect on femur BMC and biomechanical strength properties among male rats compared with females. This may be because male rats experienced greater overall body growth, as assessed by weight gain, than female rats, and suggests that the nutrient composition of the western-style diet did not support the development of strong femurs.

Keywords

Western-style dietBiomechanical bone strengthBone massRats
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 3 , September 2003 , pp. 589 - 595
Copyright
Copyright © The Nutrition Society 2003

References

Alexy, U, Sichert-Hellert, W & Kersting, M (2002) Fifteen-year time trends in energy and macronutrient intake in German children and adolescents: results of the DONALD study. Br J Nutr 87, 595–604.Google Scholar
Anderson, JJB, Sell, ML, Garner, SC & Calvo, MS (2001) Phosphorus. In Present Knowledge in Nutrition, 8th ed. pp. 281–291 [Bowman, BR and Russell, RM, editors]. Washington, DC: ILSI Press.Google Scholar
Atkinson, SA & Ward, WE (2001) Clinical nutrition: 2. The role of nutrition in the prevention and treatment of adult osteoporosis. CMAJ 165, 1511–1514.Google ScholarPubMed
Bacon, WE, Maggi, S, Looker, A, et al. (1996) International comparison of hip fracture rates in 1988–89. Osteoporos Int 6, 69–75.CrossRefGoogle ScholarPubMed
Becker, W (1999) Dietary guidelines and patterns of food and nutrient intake in Sweden. Br J Nutr 81, S113–S117.Google Scholar
Bielaczyc, AR, Golebiewska, M, Citko, A & Rogowski, F (1997) Concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum of young growing rats fed a low calcium and vitamin D-deficient diet. Eur J Clin Chem Clin Biochem 35, 915–918.Google Scholar
Binkley, N & Krueger, D (2002) Osteoporosis in men. WMJ 101, 28–32.Google Scholar
Borody, WL, Brown, TE & Boroditsky, RS (1998) Dietary fat and calcium intakes of menopausal women. Menopause 5, 230–235.Google Scholar
Brown, JP & Josse, RG (2002) 2002 clinical practice guidelines for the diagnosis and management of osteoporosis in Canada. CMAJ 167, S1–S34.Google Scholar
Burr, DB (2002) The contribution of the organic matrix to bone's material properties. Bone 31, 8–11.Google Scholar
Canadian Council on Animal Care (1984) Guide to the Care and Use of Experimental Animals. Institute of Laboratory Animal Resources Commission on Life Sciences. Ottawa, Canada: Canadian Council on Animal Care.Google Scholar
Cavadini, C, Siega-Riz, AM & Popkin, BM (2000) US adolescent food intake trends from 1965 to 1996. Arch Dis Child 83, 18–24.Google Scholar
Choumenkovitch, SF, Selhub, J, Wilson, PW, Rader, JI, Rosenberg, IH & Jacques, PF (2002) Folic acid intake from fortification in United States exceeds predictions. J Nutr 132, 2792–2798.CrossRefGoogle ScholarPubMed
Creedon, A & Cashman, KD (2001) The effect of calcium intake on bone composition and bone resorption in the young growing rat. Br J Nutr 86, 453–459.Google Scholar
Cummings, SR & Melton, LJ (2002) Epidemiology and outcomes of osteoporotic fractures. Lancet 359, 1761–1767.Google Scholar
Dawson-Hughes, B (2001) Osteoporosis. In Present Knowledge in Nutrition, 8th ed. pp. 564–572 [Bowman, BR and Russell, RM, editors]. Washington DC: ILSI Press.Google Scholar
Ejersted, C, Andreassen, TT, Oxlund, H, et al. (1993) Human parathyroid hormone (1–34) and (1–84) increase the mechanical strength and thickness of cortical bone in rats. J Bone Miner Res 8, 1097–1101.CrossRefGoogle ScholarPubMed
Food and Nutrition Board (1997) Dietary Reference Intakes for Calcium, Magnesium, Phosphorus, Vitamin D and Fluoride. Washington, DC: National Academy Press.Google Scholar
Geng, W, DeMoss, DL & Wright, GL (2000) Effect of calcium stress on the skeleton mass of intact and ovariectomized rats. Life Sci 66, 2309–2321.Google Scholar
Geng, W & Wright, GL (2001) Skeletal sensitivity to dietary calcium deficiency is increased in the female compared with the male rat. Can J Physiol Pharmacol 79, 379–385.Google Scholar
Gray-Donald, K, Jacobs-Starkey, L & Johnson-Down, L (2000) Food habits of Canadians: reduction in fat intake over a generation. Can J Public Health 91, 381–385.Google Scholar
Hannon, EM, Kiely, M, Harrington, KE, Robson, PJ, Strain, JJ & Flynn, A (2001) The North/South Ireland Food Consumption Survey: mineral intakes in 18–64–year-old adults. Public Health Nutr 4, 1081–1088.Google Scholar
Ho, SC, Bacon, WE, Harris, T, Looker, A & Maggi, S (1993) Hip fracture rates in Hong Kong and the United States, 1988 through 1989. Am J Public Health 83, 694–697.Google Scholar
Ho, SC, Lau, EM, Woo, J, et al. (1999) The prevalence of osteoporosis in the Hong Kong Chinese female population. Maturitas 32, 171–178.CrossRefGoogle ScholarPubMed
Horn, M, Kadgien, M, Schnackerz, K & Neubauer, S (2000) 31P-nuclear magnetic resonance spectroscopy of blood: a species comparison. J Cardiovasc Magn Reson 2, 143–149.Google Scholar
Kaastad, TS, Reikeras, O, Halvorsen, V, Falch, JA, Obrant, KJ & Nordsletten, L (2001) Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats. Calcif Tissue Int 69, 102–108.CrossRefGoogle ScholarPubMed
Khan, N, Yang, K, Newmark, H, et al. (1994) Mammary ductal epithelial cell hyperproliferation and hyperplasia induced by a nutritional stress diet containing four components of a western-style diet. Carcinogenesis 15, 2645–2648.Google Scholar
Koh, LK (2002) An Asian perspective to the problem of osteoporosis. Ann Acad Med Singapore 31, 26–29.Google Scholar
Krumdieck, CL & Prince, CW (2000) Mechanisms of homocysteine toxicity on connective tissues: implications for the morbidity of aging. J Nutr 130, 365S–368S.Google Scholar
Li, KC, Zernicke, RF, Barnard, RJ & Li, AF (1990) Effects of a high fat-sucrose diet on cortical bone morphology and biomechanics. Calcif Tissue Int 47, 308–313.CrossRefGoogle ScholarPubMed
Lubec, B, Fang-Kircher, S, Lubec, T, Blom, HJ & Boers, GH (1996) Evidence for McKusick's hypothesis of deficient collagen cross-linking in patients with homocystinuria. Biochim Biophys Acta 1315, 159–162.CrossRefGoogle ScholarPubMed
Melton, LJ, Chrischilles, EA, Cooper, C, Lane, AW & Riggs, BL (1992) Perspective. How many women have osteoporosis? J Bone Miner Res 7, 1005–1010.Google ScholarPubMed
Millen, BE, Quatromoni, PA, Gagnon, DR, Cupples, LA, Franz, MM & D'Agostino, RB (1996) Dietary patterns of men and women suggest targets for health promotion: the Framingham Nutrition Studies. Am J Health Promot 11, 42–52.CrossRefGoogle ScholarPubMed
Munoz, KA, Krebs-Smith, SM, Ballard-Barbash, R & Cleveland, LE (1997) Food intakes of US children and adolescents compared with recommendations. Pediatrics 100, 323–329.CrossRefGoogle ScholarPubMed
Newmark, HL, Yang, K, Lipkin, M, et al. (2001) A Western-style diet induces benign and malignant neoplasms in the colon of normal C57Bl/6 mice. Carcinogenesis 22, 1871–1875.CrossRefGoogle ScholarPubMed
Peacock, M, Turner, CH, Econs, MJ & Foroud, T (2002) Genetics of osteoporosis. Endocr Rev 23, 303–326.Google Scholar
Pettifor, JM, Marie, PJ, Sly, MR, et al. (1984) The effect of differing dietary calcium and phosphorus contents on mineral metabolism and bone histomorphometry in young vitamin D-replete baboons. Calcif Tissue Int 36, 668–676.Google Scholar
Ray, JG, Vermeulen, MJ, Boss, SC & Cole, DE (2002) Declining rate of folate insufficiency among adults following increased folic acid food fortification in Canada. Can J Public Health 93, 249–253.Google Scholar
Richter, F, Newmark, HL, Richter, A, Leung, D & Lipkin, M (1995) Inhibition of Western-diet induced hyperproliferation and hyperplasia in mouse colon by two sources of calcium. Carcinogenesis 16, 2685–2689.CrossRefGoogle ScholarPubMed
Risio, M, Lipkin, M, Newmark, H, et al. (1996) Apoptosis, cell replication, and Western-style diet-induced tumorigenesis in mouse colon. Cancer Res 56, 4910–4916.Google ScholarPubMed
Risio, M, Sarotto, I, Rossini, FP, Newmark, H, Yang, K & Lipkin, M (2000) Programmed cell death, proliferating cell nuclear antigen and p53 expression in mouse colon mucosa during diet-induced tumorigenesis. Anal Cell Pathol 21, 87–94.Google Scholar
Salem, GJ, Zernicke, RF & Barnard, RJ (1992) Diet-related changes in mechanical properties of rat vertebrae. Am J Physiol 262, R318–R321.Google ScholarPubMed
Schwartz, AV, Kelsey, JL, Maggi, S, et al. (1999) International variation in the incidence of hip fractures: cross-national project on osteoporosis for the World Health Organization Program for Research on Aging. Osteoporos Int 9, 242–253.Google Scholar
Starkey, LJ, Johnson-Down, L & Gray-Donald, K (2001) Food habits of Canadians: comparison of intakes in adults and adolescents to Canada's food guide to healthy eating. Can J Diet Pract Res 6, 61–69.Google Scholar
Tanaka, M, Nakaya, S, Kumai, T, Watanabe, M, Matsumoto, N & Kobayashi, S (2001) Impaired testicular function in rats with diet-induced hypercholesterolemia and/or streptozotocin-induced diabetes mellitus. Endocr Res 27, 109–117.Google Scholar
Thomas, ML, Simmons, DJ, Kidder, L & Ibarra, MJ (1991) Calcium metabolism and bone mineralization in female rats fed diets marginally sufficient in calcium: effects of increased dietary calcium intake. Bone Miner 12, 1–14.Google Scholar
Volatier, JL & Verger, P (1999) Recent national French food and nutrient intake data. Br J Nutr 81, S57–S59.Google Scholar
Ward, WE, Yuan, YV, Cheung, AM & Thompson, LU (2001 a) Exposure to flaxseed and its purified lignan reduces bone strength in young but not older male rats. J Toxicol Environ Health A 63, 53–65.CrossRefGoogle Scholar
Ward, WE, Yuan, YV, Cheung, AM & Thompson, LU (2001 b) Exposure to purified lignan from flaxseed (Linum usitatissimum) alters bone development in female rats. Br J Nutr 86, 499–505.Google Scholar
Wohl, GR, Loehrke, L, Watkins, BA & Zernicke, RF (1998) Effects of high-fat diet on mature bone mineral content, structure, and mechanical properties. Calcif Tissue Int 63, 74–79.Google Scholar
Xue, L, Lipkin, M, Newmark, H & Wang, J (1999) Influence of dietary calcium and vitamin D on diet-induced epithelial cell hyperproliferation in mice. J Natl Cancer Inst 91, 176–181.Google Scholar
Xue, L, Newmark, H, Yang, K & Lipkin, M (1996) Model of mouse mammary gland hyperproliferation and hyperplasia induced by a western-style diet. Nutr Cancer 26, 281–287.Google Scholar
Zernicke, RF, Salem, GJ, Barnard, RJ & Schramm, E (1995) Long-term, high-fat-sucrose diet alters rat femoral neck and vertebral morphology, bone mineral content, and mechanical properties. Bone 16, 25–31.Google Scholar