Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T03:22:59.350Z Has data issue: false hasContentIssue false
  • English
  • Français

Insulin-like growth factor-I and analogues increase growth in artificially-reared neonatal pigs

Published online by Cambridge University Press:  09 March 2007

Frank R. Dunshea ,
Chung S. Chung ,
Phil C. Owens ,
John F. Ballard  and
Paul E. Walton
Frank R. Dunshea*
Affiliation:
Victorian Institute of Animal Science, 600 Sneydes Rd., Werribee, Victoria 3030, Australia
Chung S. Chung
Affiliation:
Department of Animal Science, Chungbuk Natl U, Kaesin-Dong San 48, Cheongju, 360-763, Republic of Korea
Phil C. Owens
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, Adelaide 5000, Australia
John F. Ballard
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, Adelaide 5000, Australia
Paul E. Walton
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, Adelaide 5000, Australia
*
*Corresponding author:Dr Frank R. Dunshea, fax +61 3 9742 0400, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Exogenous insulin-like growth factor (IGF)-I has been shown to increase growth rate in neonatal pigs while an analogue of IGF-I, long arginine (LR3) IGF-I, has been shown to be more potent than IGF-I in the rat. Therefore, two studies were conducted to determine whether IGF-I and LR3IGF-I increase growth in the artificially-reared neonatal pig. Expt 1 involved forty-two (2 kg initial weight) pigs infused with either control, IGF-I (2, 4 or 8 μg/h) or LR3IGF-I (2, 4 or 8 μg/h) infusions for 8 d. Pigs were weighed and then offered 1·7 MJ (gross energy) milk replacer/kg0·75 per d. Expt 2 involved eighteen pigs (2 kg initial weight) treated with control saline, IGF-I (8 μg/h) or LR3IGF-I (8 μg/h) infusions. After 9 d an additional pump was inserted to increase the infusion rates of each of the growth factors (16 μg/h) for a further 9 d. Cows' milk was provided ad libitum. In Expt 1 there was no overall effect of growth factors on daily weight gain or slaughter weight. However, milk intake was greater in pigs infused with growth factors (909 v. 867 g/d, P=0·027), with an apparently greater milk intake by the pigs infused with IGF-I compared with LR3IGF-I (920 v. 898 g/d, P=0·12). Infusion of LR3IGF-I decreased plasma IGF-I concentrations, but had no effect on plasma IGF-II concentrations. In Expt 2, neither IGF-I nor LR3IGF-I infusion had any effect upon daily weight gain over the first 9 d of the study. However, over the second 9 d of the study, daily weight gain was increased in LR3IGF-I-infused pigs (457 v. 386 g/d, P<0·01), but not in pigs infused with IGF-I (413 v. 386 g/d, P=0·15). Milk intake was not different during the first 9 d of the study but was significantly greater in pigs infused with growth factors over the second half of the study (3407 v. 2905 g/d, P<0·01). Plasma IGF-binding protein-3 concentrations were highly correlated (R=0·85) with average daily gain over the 3 d preceding blood sampling. In conclusion, exogenous IGF-I and particularly LR3IGF-I can increase growth rate and milk intake in artificially-reared pigs fed ad libitum but not in limit-fed piglets.

Keywords

Insulin-like growth factor-IGrowthLactationSomatotropinPig
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 6 , June 2002 , pp. 587 - 593
Copyright
Copyright © The Nutrition Society 2002

References

Auldist, DE, Stevenson, FL, Kerr, MG, Eason, P & King, RH (1997) Lysine requirements of pigs from 2 to 7 kg liveweight. Animal Science 65, 501–507.CrossRefGoogle Scholar
Baxter, RC & Martin, JL (1986) Radioimmunoassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. Journal of Clinical Investigation 78, 1504–1512.CrossRefGoogle ScholarPubMed
Boyd, DR, Kensinger, RS, Harrell, RJ & Bauman, DE (1995) Nutrient uptake and endocrine regulation of milk synthesis in mammary tissue of lactating sows. Journal of Animal Science 73, Suppl. 2, 36–56CrossRefGoogle Scholar
Buonomo, FC & Klindt, J (1993) Ontogeny of growth hormone (GH), insulin-like growth factors (IGF-I and IGF-II) and IGF binding protein-2 (IGFBP-2) in genetically lean and obese swine. Domestic Animal Endocrinology 10, 257–265.CrossRefGoogle ScholarPubMed
Carr, JM, Owens, JA, Grant, PA, Owens, PC & Wallace, JC (1995) Circulating insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs) and tissue mRNA levels of IGFBP-2 and IGFBP-4 in the ovine fetus. Journal of Endocrinology 145, 545–557.CrossRefGoogle ScholarPubMed
Duchamp, C, Butron, KA, Herpin, P & Dauncey, MJ (1996) Perinatal ontogeny of porcine growth hormone receptor gene expression is modulated by thyroid status. European Journal of Endocrinology 134, 524–531.CrossRefGoogle ScholarPubMed
Dunaiski, V, Dunshea, FR & Goddard, C (1997) Regulation of insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) mRNA by GH in different porcine tissues. Journal of Endocrinology 155, 559–565.CrossRefGoogle Scholar
Dunshea, FR, King, RH, Owens, PC & Walton, PE (1999) Moderate doses of porcine somatotropin do not increase plasma insulin-like growth factor-I or insulin-like growth-factor binding protein-3. Domestic Animal Endocrinology 16, 149–157.CrossRefGoogle Scholar
Francis, GL, Owens, PC, McNeil, KA, Wallace, JC & Ballard, FJ (1989) Purification, amino-acid sequences and assay cross-reactivities of porcine insulin-like growth factor I and II. Journal of Animal Science 72, Suppl. 1, 253Google Scholar
Giordano, M & DeFronzo, RA (1995) Acute effect of human recombinant insulin-like growth factor I on renal function in humans. Nephron 71, 10–15.CrossRefGoogle ScholarPubMed
Harrell, RJ, Thomas, MJ, Boyd, RD, Bauman, DE, Czerwinski, S & Steele, NC (1997) Ontogenic dependent response to exogenous porcine somatotropin in growing pigs. Journal of Animal Science 77, Suppl. 1, 253Google Scholar
Holl, RW, Snehotta, R, Siegler, B, Scherbaum, W & Heinze, E (1991) Binding protein for hormone: effects of age and weight. Hormone Research 35, 190–197.CrossRefGoogle ScholarPubMed
Malmloff, K, Cortova, Z, Saxerhalt, H, Karlsson, E & Skottner, A (1994) IGF-I and GH: Metabolic effects during experimentally induced catabolism. Growth Regulation 4, Suppl. 1, 51Google Scholar
Martin, AA, Tomas, FM, Owens, PC, Knowles, SE, Ballard, FJ & Read, LC (1991) IGF-I and its variant, des-(1–3)IGF-I, enhance growth in rats with reduced renal mass. American Journal of Physiology 261, F626–F633.Google ScholarPubMed
Matteri, RL & Carroll, JA (1997) Somatotroph function in the neonatal pig. Domestic Animal Endocrinology 4, 241–249.Google Scholar
Payne, RW, Lane, PW & Genstat 5 Committee (1993) Genstat 5 Reference Manual. Oxford: Oxford Science Publications.CrossRefGoogle Scholar
Owens, PC, Campbell, RG, Francis, GL & Quinn, KJ (1994) Growth hormone, gender and insulin-like growth factors: relationship to growth performance in pigs. Journal of Animal Science 72, Suppl. 1, 253Google Scholar
Owens, PC, Campbell, RG & Luxford, BG (1997) Experimental correlations between insulin-like growth factors (IGF's) and growth rate show that endocrine IGF's are growth reporters, not drivers. In Manipulating Pig Production VI, p. 171 [Cranwell, PD, editor]. Werribee, Victoria: APSA.Google Scholar
Owens, PC, Gatford, KL, Walton, PE, Morley, W & Campbell, RG (1999) The relationship between endogenous insulin-like growth factors and growth in pigs. Journal of Animal Science 77, 2098–2103.CrossRefGoogle ScholarPubMed
Owens, PC, Johnson, RJ, Campbell, RG & Ballard, FJ (1990) Growth hormone increases insulin-like growth factor-I (IGF-I) and decreases IGF-II in plasma of growing pigs. Journal of Endocrinology 124, 269–275.CrossRefGoogle ScholarPubMed
Schnoelbelen-Combes, S, Louveau, I, Postel-Vinay, MC & Bonneau, M (1996) Ontogeny of GH receptor and GH-binding protein in the pig. Journal of Endocrinology 148, 249–255.CrossRefGoogle Scholar
Schoknecht, PA, Ebner, S, Skottner, A, Burrin, DG, Davis, TA, Ellis, K & Pond, WG (1997) Exogenous insulin-like growth factor-I increases weight gain in intrauterine growth-retarded neonatal pigs. Pediatric Research 42, 201–207.CrossRefGoogle ScholarPubMed
Thissen, JP, Davenport, ML, Pucilowska, JB, Miles, MV & Underwood, LE (1992) Increased serum clearance and degradation of 125I-labeled IGF-I in protein-restricted rats. American Journal of Physiology 262, E406–E411.Google ScholarPubMed
Tomas, FM, Knowles, SE, Chandler, CS, Francis, GL, Owens, PC & Ballard, FJ (1993) Anabolic effects of insulin-like growth factor-I (IGF-I) and an IGF-I variant in normal female rats. Endocrinology 137, 413–421.CrossRefGoogle Scholar
Tomas, FM, Knowles, SE, Owens, PC, Chandler, CS, Francis, GL, Read, LC & Ballard, FJ (1992) Insulin-like growth factor-I (IGF-I) and especially IGF-I variants are anabolic in dexamethasone-treated rats. Biochemical Journal 282, 91–97.CrossRefGoogle ScholarPubMed
Walton, PE & Etherton, TD (1989) Effects of porcine growth hormone and insulin-like growth factor-I (IGF-I) on immunoreactive IGF-binding protein concentration in pigs. Journal of Endocrinology 120, 153–160.CrossRefGoogle ScholarPubMed
Walton, PE, Dunshea, FR & Ballard, FJ (1995) In vivo actions of IGF analogues with poor affinities for IGFBPs: metabolic and growth effects in pigs of different ages and GH responsiveness. Progress in Growth Factor Research 6, 385–395.CrossRefGoogle ScholarPubMed