Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-07T13:27:43.776Z Has data issue: false hasContentIssue false

Enteral administration of soyabean lecithin enhanced lymphatic absorption of triacylglycerol in rats

Published online by Cambridge University Press:  09 March 2007

Megumi Nishimukai
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan
Hiroshi Hara*
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan
Yoritaka Aoyama
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan
*
*Corresponding author:Dr Hiroshi Hara, fax +81 11 706 2504, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

As the physiological roles of dietary lecithin have not yet been clearly defined, we examined the effects of lecithin on lipid absorption in male Wistar rats with a mesenteric lymph cannula. Lymphatic absorption was observed after the infusion of 1 ml emulsion containing 100 mg test oil emulsified with sodium taurocholate (10 g/l) in three separate experiments. Test oils (100 mg) were: soyabean oil (triacylglycerol (TG) source, SO) and soyabean oil + lecithin (75 mg soyabean oil+25 mg lecithin, LE) in Expt 1; SO, LE or soyabean oil + lysolecithin (75 mg soyabean oil plus 25 mg lysolecithin, LY) in Expt 2; hydrolysed soyabean oil (HSO) or HSO+lysolecithin (75 mg HSO+25 mg lysolecithin, HLY) in Expt 3. After LE and LY infusions, lymph flow and the lymphatic output of TG was higher than after SO infusion at 0-30 min and 0-90 min respectively (Expts 1 and 2). Lecithin-induced increases in lymph TG output remained constant when HSO was infused (Expt 3). There were no differences in the TG:phospholipid ratio in the lymph after infusion among the groups; nevertheless, the lymphatic output of TG was much higher after infusion with LE than with SO. Fatty acid was released more efficiently from SO than from LE and LY by in vitro digestion with rat bile–pancreatic juice. These present results demonstrate that a TG emulsion containing soyabean lecithin or its hydrolysates promote lymphatic TG output and suggest that the increases in TG absorption do not depend on TG digestion.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2003

References

Ahn, J & Koo, SI (1995) Intraduodenal phosphatidylcholine infusion restores the lymphatic absorption of vitamin A and oleic acid in zinc-deficient rats. J Nutr Biochem 6, 604612.CrossRefGoogle Scholar
Alexander, JW (1998) Immunonutrition: the role of omega-3 fatty acids. Nutrition 14, 627633.CrossRefGoogle ScholarPubMed
Bamba, T, Shimoyama, T, Sasaki, M, et al. (2003) Dietary fat attenuates the benefits of an elemental diet in active Crohn's disease: a randomized, controlled trial. Eur J Gastroenterol Hepatol 15, 151157.Google Scholar
Bohlen, HG & Unthank, JL (1989) Rat intestinal lymph osmolarity during glucose and oleic acid absorption. Am J Physiol 257, G438G446.Google ScholarPubMed
Bollman, JL, Cain, JC & Grindlay, JH (1948) Techniques for the collection of lymph from the liver, small intestine or thoracic duct of the rat. J Lab Clin Med 33, 13491352.Google Scholar
Borgström, B (1980) Importance of phospholipids, pancreatic phospholipase A2, and fatty acid for the digestion of dietary fat: in vitro experiments with the porcine enzymes. Gastroenterology 78, 954962.Google Scholar
Davidson, NO, Kollmer, ME & Glickman, RM (1986) Apolipoprotein B synthesis in rat small intestine: regulation by dietary triglyceride and biliary lipid. J Lipid Res 27, 3039.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497509.CrossRefGoogle ScholarPubMed
Granger, DN, Korthuis, RJ, Kvietys, PR & Tso, P (1988) Intestinal microvascular exchange during lipid absorption. Am J Physiol 255, G690G695.Google ScholarPubMed
Hamilton, RL, Wong, JS, Cham, CM, Nielsen, L & Young, SG (1998) Chylomicron-sized lipid particles are formed in the setting of apolipoprotein B deficiency. J Lipid Res 39, 15431557.Google Scholar
Hollander, D & Morgan, D (1980) Effect of plant sterols, fatty acids and lecithin on cholesterol absorption in vivo in the rat. Lipids 15, 395400.Google Scholar
Homan, R & Hamelehle, KL (1998) Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-2. J Lipid Res 39, 11971209.CrossRefGoogle ScholarPubMed
Jiang, Y, Noh, SK & Koo, SI (2001) Egg phosphatidylcholine decreases the lymphatic absorption of cholesterol in rats. J Nutr 131, 23582363.Google Scholar
Koo, SI & Noh, SK (2001) Phosphatidylcholine inhibits and lysophosphatidylcholine enhances the lymphatic absorption of alpha-tocopherol in adult rats. J Nutr 131, 717722.Google Scholar
Le Kim, D & Betzing, H (1976) Intestinal absorption of polyunsaturated phosphatidylcholine in the rat. Hoppe Seylers Z Physiol Chem 357, 13211331.Google Scholar
Luddy, FE, Barford, RA, Herb, SF, Magidman, P & Riemenschneider, RW (1964) Pancreatic lipase hydrolysis of triglycerides by a semimicro technique. J Am Oil Chem Soc 41, 693696.Google Scholar
Murota, K, Matsui, N, Kawada, T, Takahashi, N & Fushuki, T (2001) Inhibitory effect of monoacylglycerol on fatty acid uptake into rat intestinal epithelial cells. Biosci Biotechnol Biochem 65, 14411443.Google Scholar
Noh, SK & Koo, SI (1997) The lymphatic absorption of lipids is normalized by enteral phosphatidylcholine infusion in ovariectomized rats with estrogen replacement. J Nutr Biochem 8, 152161.Google Scholar
Noh, SK & Koo, SI (2001) Enteral infusion of phosphatidylcholine increases the lymphatic absorption of fat, but lowers alpha-tocopherol absorption in rats fed a low zinc diet. J Nutr Biochem 12, 330337.CrossRefGoogle ScholarPubMed
O'Doherty, PJ, Kakis, G & Kuksis, A (1973) Role of luminal lecithin in intestinal fat absorption. Lipids 8, 249255.CrossRefGoogle ScholarPubMed
Reynier, MO, Lafont, H, Crotte, C, Sauve, P & Gerolami, A (1985) Intestinal cholesterol uptake: comparison between mixed micelles containing lecithin or lysolecithin. Lipids 20, 145150.CrossRefGoogle ScholarPubMed
Tso, P, Ding, K, DeMichele, S & Huang, YS (2002) Intestinal absorption and lymphatic transport of a high gamma-linolenic acid canola oil in lymph fistula Sprague–Dawley rats. J Nutr 132, 218221.CrossRefGoogle ScholarPubMed
Tso, P, Kendrick, H, Balint, JA & Simmonds, WJ (1981) Role of biliary phosphatidylcholine in the absorption and transport of dietary triolein in the rat. Gastroenterology 80, 6065.Google Scholar
van Greevenbroek, MM, Robertus-Teunissen, MG, Erkelens, DW & de, Bruin TW (1998) Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in Caco-2 cells: interaction with saturated and unsaturated dietary fatty acids. J Lipid Res 39, 173185.Google Scholar
Wang, H, Du, J, Lu, S, Yao, Y, Hunter, F & Black, DD (2001) Regulation of intestinal apolipoprotein A-I synthesis by dietary phosphatidylcholine in newborn swine. Lipids 36, 683687.Google Scholar