No CrossRef data available.
Article contents
Effect of moderate wine consumption on the activity of enzymes involved in platelet-activating factor metabolism and thrombotic biomarkers: a randomised, single-blind, parallel, clinical study in CHD men patients
Published online by Cambridge University Press: 13 February 2025
Abstract
A randomised parallel intervention study was conducted with male patients diagnosed with CHD. Participants were assigned to three groups: Group A abstained from alcohol (n 20), Group B consumed red wine (n 21) and Group C (n 16) consumed an alcoholic beverage without wine micro-constituents. Biological samples were collected at baseline, 4 and 8 weeks. Enzyme activities of acetyl-CoA:lyso-platelet-activating factor (PAF) acetyltransferase, cytidine 5’-diphospho (CDP)-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-cholinephosphotransferase), PAF-acetylhydrolase in leukocyte homogenates, serum lipoprotein-associated phospholipase-A2 and plasma markers of thrombosis were measured. PAF-, ADP- and collagen-induced platelet aggregation was measured in human platelet-rich plasma. Red wine consumption led to a 15·3 % reduction in LysoPAF-acetyltransferase activity at 4 weeks (P= 0·008) compared with baseline and Group A (P= 0·01). PAF-cholinephosphotransferase activity was reduced by 11·1 % at 8 weeks (P= 0·04) compared with baseline and by 24·9 % compared with Group C (P= 0·02). PAF-acetylhydrolase activity was reduced by 36·2 % at 8 weeks compared with baseline (P= 0·001) and compared with Group A (P< 0·000) and Group C (P= 0·009). Fibrinogen levels in Group B reduced by 6–9 % at 4 (P= 0·04) and 8 weeks (P= 0·01) compared with baseline while D-dimer in Group C increased by 16·1 % at 8 weeks (P= 0·005) compared with baseline. Platelet aggregation against PAF and collagen was reduced in Group B (82·6 and 35·4 %, respectively), and in Group C (158·4 and 37·1 %, respectively) compared with baseline and Group A (P< 0·05). In conclusion, moderate wine consumption improved the activity of PAF-metabolism enzymes regardless of ethanol and reduced platelet aggregation, probably through mechanisms different from those of ethanol.
- Type
- Research Article
- Information
- Copyright
- © The Author(s), 2025. Published by Cambridge University Press on behalf of The Nutrition Society
References
Bruins, MJ, Van Dael, P & Eggersdorfer, M (2019) The role of nutrients in reducing the risk for noncommunicable diseases during aging. Nutrients 11, 85.Google Scholar
Mozaffarian, D (2016) Dietary and policy priorities for cardiovascular disease, diabetes, and obesity: a comprehensive review. Circulation 133, 187–225.Google Scholar
Costanzo, S, Di Castelnuovo, A, Donati, MB, et al. (2011) Wine, beer or spirit drinking in relation to fatal and non-fatal cardiovascular events: a meta-analysis. Eur J Epidemiol 26, 833–850.Google Scholar
Visseren, FLJ, Mach, F, Smulders, YM, et al. (2021) 2021 ESC Guidelines on cardiovascular disease prevention in clinical practice. Eur Heart J 42, 3227–3337.Google Scholar
O’Keefe, EL, DiNicolantonio, JJ, O’Keefe, JH, et al. (2018) Alcohol and CV health: Jekyll and Hyde J-Curves. Prog Cardiovasc Dis 61, 68–75.Google Scholar
Fragopoulou, E & Antonopoulou, S (2020) The French paradox three decades later: role of inflammation and thrombosis. Clin Chim Acta 510, 160–169.Google Scholar
Fragopoulou, E, Demopoulos, CA & Antonopoulou, S (2009) Lipid minor constituents in wines. A biochemical approach in the French paradox. Int J Wine Res 1, 131–143.Google Scholar
Vallverdú-Queralt, A, Boix, N, Piqué, E, et al. (2015) Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS. Food Chem 181, 146–151.Google Scholar
Demopoulos, CA, Karantonis, HC & Antonopoulou, S (2003) Platelet activating factor— a molecular link between atherosclerosis theories. Eur J Lipid Sci Technol 105, 705–716.Google Scholar
Snyder, F (1995) Platelet-activating factor and its analogs: metabolic pathways and related intracellular processes. Biochim Biophys Acta 1254, 231–249.Google Scholar
Shindou, H, Hishikawa, D, Nakanishi, H, et al. (2007) A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells. Cloning and characterization of acetyl-CoA:LYSO-PAF acetyltransferase. J Biol Chem 282, 6532–6539.Google Scholar
Snyder, F (1997) CDP-choline:alkylacetylglycerol cholinephosphotransferase catalyzes the final step in the de novo synthesis of platelet-activating factor. Biochim Biophys Acta 1348, 111–116.Google Scholar
Stafforini, DM (2009) Biology of platelet-activating factor acetylhydrolase (PAF-AH, Lipoprotein Associated Phospholipase A2). Cardiovasc Drugs Ther 23, 73–83.Google Scholar
Zimmerman, GA, McIntyre, TM, Prescott, SM, et al. (2002) The platelet-activating factor signaling system and its regulators in syndromes of inflammation and thrombosis. Crit Care Med 30, S294–S301.Google Scholar
Verouti, SN, Fragopoulou, E, Karantonis, HC, et al. (2011) PAF effects on MCP-1 and IL-6 secretion in U-937 monocytes in comparison with OxLDL and IL-1β effects. Atherosclerosis 219, 519–525.Google Scholar
Fragopoulou, E, Antonopoulou, S & Demopoulos, CA (2002) Biologically active lipids with antiatherogenic properties from white wine and must. J Agric Food Chem 50, 2684–2694.Google Scholar
Fragopoulou, E, Antonopoulou, S, Nomikos, T, et al. (2003) Structure elucidation of phenolic compounds from red/white wine with antiatherogenic properties. Biochim Biophys Acta 1632, 90–99.Google Scholar
Xanthopoulou, MN, Asimakopoulos, D, Antonopoulou, S, et al. (2014) Effect of Robola and Cabernet Sauvignon extracts on platelet activating factor enzymes activity on U937 cells. Food Chem 165, 50–59.Google Scholar
Vlachogianni, IC, Fragopoulou, E, Stamatakis, GM, et al. (2015) Platelet Activating Factor (PAF) biosynthesis is inhibited by phenolic compounds in U-937 cells under inflammatory conditions. Prostaglandins Other Lipid Mediat 121, 176–183.Google Scholar
Angel-Morales, G, Noratto, G & Mertens-Talcott, S (2012) Red wine polyphenolics reduce the expression of inflammation markers in human colon-derived CCD-18Co myofibroblast cells: potential role of microRNA-126. Food Funct 3, 745.Google Scholar
Calabriso, N, Scoditti, E, Massaro, M, et al. (2016) Multiple anti-inflammatory and anti-atherosclerotic properties of red wine polyphenolic extracts: differential role of hydroxycinnamic acids, flavonols and stilbenes on endothelial inflammatory gene expression. Eur J Nutr 55, 477–489.Google Scholar
Chalons, P, Amor, S, Courtaut, F, et al. (2018) Study of potential anti-inflammatory effects of red wine extract and resveratrol through a modulation of interleukin-1-beta in macrophages. Nutrients 10, 1856.Google Scholar
Fragopoulou, E, Choleva, M, Antonopoulou, S, et al. (2018) Wine and its metabolic effects. A comprehensive review of Clinical Trials. Metabolism 83, 102–119.Google Scholar
Chiva-Blanch, G, Urpi-Sarda, M, Llorach, R, et al. (2012) Differential effects of polyphenols and alcohol of red wine on the expression of adhesion molecules and inflammatory cytokines related to atherosclerosis: a randomized clinical trial. Am J Clin Nutr 95, 326–334.Google Scholar
Vázquez-Agell, M, Sacanella, E, Tobias, E, et al. (2007) Inflammatory markers of atherosclerosis are decreased after moderate consumption of cava (sparkling wine) in men with low cardiovascular risk. J Nutr 137, 2279–2284.Google Scholar
Estruch, R, Sacanella, E, Badia, E, et al. (2004) Different effects of red wine and gin consumption on inflammatory biomarkers of atherosclerosis: a prospective randomized crossover trial. Effects of wine on inflammatory markers. Atherosclerosis 175, 117–123.Google Scholar
Karatzi, K, Papamichael, C, Aznaouridis, K, et al. (2004) Constituents of red wine other than alcohol improve endothelial function in patients with coronary artery disease. Coron Artery Dis 15, 485–490.Google Scholar
Agewall, S (2000) Does a glass of red wine improve endothelial function? Eur Heart J 21, 74–78.Google Scholar
Whelan, AP, Sutherland, WHF, McCormick, MP, et al. (2004) Effects of white and red wine on endothelial function in subjects with coronary artery disease. Intern Med J 34, 224–228.Google Scholar
Argyrou, C, Vlachogianni, I, Stamatakis, G, et al. (2017) Postprandial effects of wine consumption on Platelet Activating Factor metabolic enzymes. Prostaglandins Other Lipid Mediat 130, 23–29.Google Scholar
Choleva, M, Argyrou, C, Detopoulou, M, et al. (2022) Effect of moderate wine consumption on oxidative stress markers in coronary heart disease patients. Nutrients 14, 1377.Google Scholar
Fragopoulou, E, Argyrou, C, Detopoulou, M, et al. (2021) The effect of moderate wine consumption on cytokine secretion by peripheral blood mononuclear cells: a randomized clinical study in coronary heart disease patients. Cytokine 146, 155629.Google Scholar
Bountziouka, V, Bathrellou, E, Giotopoulou, A, et al. (2012) Development, repeatability and validity regarding energy and macronutrient intake of a semi-quantitative food frequency questionnaire: methodological considerations. Nutr Metab Cardiovasc Dis 22, 659–667.Google Scholar
Panagiotakos, DB, Pitsavos, C, Arvaniti, F, et al. (2007) Adherence to the Mediterranean food pattern predicts the prevalence of hypertension, hypercholesterolemia, diabetes and obesity, among healthy adults; the accuracy of the MedDietScore. Prev Med 44, 335–340.Google Scholar
Kavouras, SA, Maraki, MI, Kollia, M, et al. (2016) Development, reliability and validity of a physical activity questionnaire for estimating energy expenditure in Greek adults. Sci Sports 31, e47–e53.Google Scholar
Detopoulou, P, Nomikos, T, Fragopoulou, E, et al. (2009) Platelet activating factor (PAF) and activity of its biosynthetic and catabolic enzymes in blood and leukocytes of male patients with newly diagnosed heart failure. Clin Biochem 42, 44–49.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.Google Scholar
Bligh, EG & Dyer, WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.Google Scholar
Little, RJ, D’Agostino, R, Cohen, ML, et al. (2012) The prevention and treatment of missing data in clinical trials. N Engl J Med 367, 1355–1360.Google Scholar
Anderson, BO, Berdzuli, N, Ilbawi, A, et al. (2023) Health and cancer risks associated with low levels of alcohol consumption. Lancet Public Health 8, e6–e7.Google Scholar
Minzer, S, Estruch, R & Casas, R (2020) Wine intake in the framework of a Mediterranean diet and chronic non-communicable diseases: a short literature review of the last 5 years. Molecules 25, 5045.Google Scholar
Boban, M, Stockley, C, Teissedre, P-L, et al. (2016) Drinking pattern of wine and effects on human health: why should we drink moderately and with meals? Food Funct 7, 2937–2942.Google Scholar
Ninio, E (2005) Phospholipid mediators in the vessel wall: involvement in atherosclerosis. Curr Opin Clin Nutr Metab Care 8, 123–131.Google Scholar
Liapikos, TA, Antonopoulou, S, Karabina, SP, et al. (1994) Platelet-activating factor formation during oxidative modification of low-density lipoprotein when PAF-acetylhydrolase has been inactivated. Biochim Biophys Acta 1212, 353–360.Google Scholar
Yanoshita, R, Chang, HW, Son, KH, et al. (1996) Inhibition of lyso PAF acetyltransferase activity by flavonoids. Inflamm Res 45, 546–549.Google Scholar
Balestrieri, ML, Castaldo, D, Balestrieri, C, et al. (2003) Modulation by flavonoids of PAF and related phospholipids in endothelial cells during oxidative stress. J Lipid Res 44, 380–387.Google Scholar
Detopoulou, P, Fragopoulou, E, Nomikos, T, et al. (2015) The relation of diet with PAF and its metabolic enzymes in healthy volunteers. Eur J Nutr 54, 25–34.Google Scholar
Swellam, M, Sayed Mahmoud, M & Abdel-Fatah Ali, A (2009) Clinical implications of adiponectin and inflammatory biomarkers in type 2 diabetes mellitus. Dis Markers 27, 269–278.Google Scholar
Wang, W-Q, Zhang, H-F, Gao, G-X, et al. (2011) Adiponectin inhibits hyperlipidemia-induced platelet aggregation via attenuating oxidative/nitrative stress. Physiol Res 60, 347–354.Google Scholar
Xanthopoulou, MN, Kalathara, K, Melachroinou, S, et al. (2017) Wine consumption reduced postprandial platelet sensitivity against platelet activating factor in healthy men. Eur J Nutr 56, 1485–1492.Google Scholar
Knight, CJ (2003) Antiplatelet treatment in stable coronary artery disease. Heart 89, 1273–1278.Google Scholar
Fragopoulou et al. supplementary material
Fragopoulou et al. supplementary material
File
53.2 KB