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The effect of bovine whey protein on ectopic bone formation in young growing rats

Published online by Cambridge University Press:  09 March 2007

Owen Kelly ,
Siobhan Cusack  and
Kevin D. Cashman
Owen Kelly
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Republic of Ireland
Siobhan Cusack
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Republic of Ireland
Kevin D. Cashman*
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Republic of Ireland Department of Medicine, University College, Cork, Republic of Ireland
*
*Corresponding author:Professor Kevin D. Cashman, fax +353 21 4270244, email [email protected]
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Abstract

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The beneficial effect of bovine whey protein (WP) on bone metabolism has been shown in adult human subjects and ovariectomised rats. However, its effect on bone formation in earlier life, particularly during periods of bone mineral accrual, has not been investigated. Twenty-one male rats (4 weeks old, Wistar strain) were randomised by weight into three groups of seven rats each and fed ad libitum on a semi-purified low-Ca diet (3·0 g Ca/kg diet) containing 0 (control), 10 (diet WP1) or 20 (diet WP2) g bovine WP/kg for 47 d. On day 34 of the dietary intervention, all rats had two gelatine capsules containing demineralised bone powder implanted subcutaneously in the thorax region (a well-established in vivo model of ectopic bone formation). At 14 d after implantation, alkaline phosphatase activity (reflective of bone formation) in the bone implants from animals fed WP1 and -2 diets was almost 2-fold (P<0·01) that of control animals. Insulin-like growth factor (IGF)-I mRNA levels were about 3-fold (P<0·05) higher in implants from animals fed the WP diets compared with those from control animals. Serum- and urine-based biomarkers of bone metabolism and bone mineral composition in intact femora were unaffected by WP supplementation. In conclusion, the present findings suggest that bovine WP can enhance the rate of ectopic bone formation in young growing rats fed a Ca-restricted diet. This effect may be mediated by an increased synthesis of IGF-I in growing bone. The effect of WP on bone formation warrants further investigation.

Keywords

Bone formationEctopic boneWhey proteinRats
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 3 , September 2003 , pp. 557 - 564
Copyright
Copyright © The Nutrition Society 2003

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