Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-26T03:45:02.873Z Has data issue: false hasContentIssue false
  • English
  • Français

The contribution of protozoa to the protein entering the duodenum of sheep

Published online by Cambridge University Press:  09 December 2008

D. G. Harrison ,
D. E. Beever  and
D. F. Osbourn
D. G. Harrison
Affiliation:
Department of Agricultural Biochemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NEI 7RU
D. E. Beever
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berkshire SL6 5LR
D. F. Osbourn
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berkshire SL6 5LR
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Four sheep, each fitted with a rumen fistula and a re-entrant cannula at the proximal duodenum were fed a semi-purified diet containing urea as the only nitrogen source. The quantities of total protozoal amino acid-N (TPAN) present in the rumen and entering the duodenum were determined when the mean rumen dilution rate (D) was low (0.034/h) and when D was increased to 0.078/h by the intraruminal infusion of artificial saliva.

2. Increasing the dilution rate had no significant effect upon the proportions of TPAN present in the total microbial amino acid-N (TMAN) of the rumen fluid and duodenal digesta. With both dilution rates the mean proportion of TPAN in the duodenal TMAN (0.24) was markedly less than the equivalent proportion (0.45) found in the rumen fluid.

3. The daily flow of TPAN, as measured at the duodenal cannula at both dilution rates was equivalent to only 41 % of the flow of TPAN as predicted from measurements of rumen outflow, indicating that a substantial proportion of rumen protozoal protein was retained within the rumen.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 41 , Issue 3 , May 1979 , pp. 521 - 527
Copyright
Copyright © The Nutrition Society 1979

References

Abe, M. & Kumeno, F. (1973). J. Anim. Sci. 36, 941.CrossRefGoogle Scholar
Beever, D. E., Harrison, D. G., Thomson, D. J., Cammell, S. B. & Osbourn, D. F. (1974). Br. J. Nutr. 32, 99.CrossRefGoogle Scholar
Beever, D. E., Thomson, D. J., Pfeffer, E. & Armstrong, D. G. (1971). Br. J. Nutr. 26, 123.CrossRefGoogle Scholar
Ben-Ghedalia, D., McMeniman, N. P. & Armstrong, D. G. (1978). Br. J. Nutr. 39, 37.CrossRefGoogle Scholar
Boyne, A. W., Eadie, J. M. & Raitt, K. (1957). J. gen. Microbiol. 17, 414.CrossRefGoogle Scholar
Brown, G.F., Armstrong, D. G. & MacRae, J. C. (1968). Br. vet. J. 124, 78.CrossRefGoogle Scholar
Clarke, E. H. W., Ellinger, B. M. & Phillipson, A. T. (1966). Proc. R. Soc. B 166, 63.Google Scholar
Coleman, G. S. (1971). Proc. Soc. gen. Microbiol. 61, 4.Google Scholar
Corbett, J. L., Greenhalgh, J. F. D., McDonald, J. & Florence, E. (1960). Br. J. Nutr. 14, 289.CrossRefGoogle Scholar
Harrison, D. G. (1974). Newsl. app. Nucl. Meth. Biol. Agric. 3, 8.Google Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1973). J. agric. Sci., Camb. 81, 391.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1975). J. agric. Sci., Camb. 85, 93.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1976). J. Sci. Fd Agric. 27, 617.CrossRefGoogle Scholar
Harrop, C. J. F. (1974). J. agric. Sci., Camb. 83, 249.CrossRefGoogle Scholar
Hungate, R.. E. (1966). The Rumen and its Microbes. New York: Academic Press.Google Scholar
Hungate, R. E., Reichl, J. & Prins, R. (1971). Appl. Microbiol. 22, 1104.CrossRefGoogle Scholar
Hutton, K., Bailey, J. F. & Annison, E. F. (1971). Br. J. Nutr. 26, 123Google Scholar
Kennedy, P. M. & Milligan, L. P. (1978). Br. J. Nutr. 39, 105.CrossRefGoogle Scholar
Ling, J. R. & Buttery, P. J. (1978). Br. J. Nutr. 39, 165.CrossRefGoogle Scholar
McDougall, E. I. (1948). Biochem. J. 43, 99.CrossRefGoogle Scholar
MacRae, J. C. & Armstrong, D. G. (1969). Br. J. Nutr. 23, 15.CrossRefGoogle Scholar
Martin, H. H. (1966). A Rev. Biochem. 35, 457.CrossRefGoogle Scholar
Mason, V.C. & White, F. (1971). J. agric. Sci., Camb. 77, 91.CrossRefGoogle Scholar
Minson, D. J. (1966). Br. J. Nutr. 20, 757CrossRefGoogle Scholar
Purser, P. B. & Buechler, S. M. (1966). J. Dairy Sci. 30, 988.Google Scholar
Rahman, S. A., Purser, D. B. & Tyznik, W. J. (1964). J. Protozool 11, 51.CrossRefGoogle Scholar
Smith, R. H. (1969). J. Dairy Res. 36, 313.CrossRefGoogle Scholar
Stevenson, A. E. & de Langen, H. (1960). N.Z. Jl agric. Res. 3, 314.CrossRefGoogle Scholar
Synge, R. L. M. (1953). J. gen. Microbiol. 9, 407.Google Scholar
Warner, A. C. I. & Stacy, B. D. (1968). Br. J. Nutr. 22, 369.CrossRefGoogle Scholar
Weller, R. A. & Pilgrim, A. F. (1974). Br. J. Nutr. 32, 341.CrossRefGoogle Scholar