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Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose

Published online by Cambridge University Press:  09 December 2008

J. R. Ebiner ,
K. J. Acheson ,
A. Doerner ,
E. Maeder ,
M. J. Arnaud ,
E. Jéquier  and
J. P. Felber
J. R. Ebiner
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
K. J. Acheson
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
A. Doerner
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
E. Maeder
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
M. J. Arnaud
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
E. Jéquier
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
J. P. Felber
Affiliation:
Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
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Abstract

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1. Carbohydrate (CHO) oxidation was measured simultaneously in a group of five normal subjects after an oral load of 100 g naturally-labelled [13C]glucose, using indirect calorimetry and mass spectrometry.

2. CHO utilization, calculated from the results of indirect calorimetry, increased 30 min after the glucose load to reach a peak at 90 min. It then decreased to reach basal values at 380 min. Cumulative total CHO oxidation at 480 min was 83±8g, and CHO oxidized above basal levels, 37±3 g.

3. Enrichment of expired carbon dioxide with 13C began at 60 min and maximum values were observed at 270 min. At 480 min, cumulative CHO oxidation measured by use of [13C]glucose was 29 g. The difference from calorimetric values can be attributed in part to the slow isotopic dilution in the glucose and bicarbonate pools.

4. Thus, approximately 30% of the glucose load was oxidized during the 8 h after its ingestion and this accounts for a significant part of the increased CHO oxidation (37 g), as measured by indirect calorimetry.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 41 , Issue 3 , May 1979 , pp. 419 - 429
Copyright
Copyright © The Nutrition Society 1979

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