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Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective

Published online by Cambridge University Press:  12 May 2014

Katja Metfies ,
Anja Nicolaus ,
Lena Von Harbou ,
Ulrich Bathmann  and
Ilka Peeken
Katja Metfies*
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
Anja Nicolaus
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Lena Von Harbou
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Ulrich Bathmann
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany Leibniz-Institute for Baltic Sea Research, Seestrasse 15, 18119 Rostock, Germany
Ilka Peeken
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany MARUM – Center for Marine Environmental Sciences, University of Bremen, 28334 Bremen, Germany
*
[email protected]
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Abstract

The diet of Antarctic salps was elucidated by investigating their gut content using automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Salp samples were collected during the Lazarev Sea Krill Study in the western Weddell Sea (summer 2005–06 and 2007–08, autumn 2004 and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai, both occur in the Southern Ocean and can overlap geographically and seasonally. We provide evidence that, despite the non-selective feeding mechanism, the two co-occurring salp species might have different niches within a habitat. ARISA-patterns of 93 gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae, constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae, differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate-based diet, even though microscopic analyses identified diatoms as the dominant component of salp gut contents.

Keywords

454-pyrosequencingARISAdietIhlea racovitzaiSalpa thompsoniSouthern Ocean
Type
Biological Sciences
Information
Antarctic Science , Volume 26 , Supplement 5 , October 2014 , pp. 545 - 553
Copyright
© Antarctic Science Ltd 2014 

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