The variation of protein fractions in the milk of indigenous Greek goats fCapra prisca) during the milking period was studied. Total nitrogen (TN), non-casein or soluble nitrogen (SN), rennet whey nitrogen (WN), non-protein nitrogen (NPN) were determined and from these analyses crude protein (CP), true protein (TP), casein (Cas) rennet casein or coagulable protein (Coag P) were calculated. The NPN component is lost during the cheese process whereas Cas and Coag P are important constituents of the cheese itself. The ratios NPNICP, CaslTP and Coag PITP throughout milking, were for the groups A and B 0·0646 and 0·0648 (s.e.d. = 0·00025), 0·846 and 0·845 (s.e.d. = 0·0141) and 0·916 and 0·904 (s.e.d. = 0·0112), respectively, suggesting a high level of suitability for conversion of milk into cheese.

With the objective of studying the response patterns of two biotypes of different body (structural) size to stocking rate (SR) in terms of individual growth rate (ADG), meat production per ha (PROD) and backfat thickness (BFT) under grazing conditions, 64 steers of two biotypes of the Angus breed of different body size were used: small-sized (SB: frame score of 1·3 (s.d. 0·7)) and medium-sized (MB: 2·9 (s.d. 0·6)). Four levels of SR were applied (1·8, 2·3, 2·8 and 3·3 steers per ha). The experiment lasted 241 days (April to December). Animals were weighed every 14 days, forage availability (FA) was estimated on four occasions and BFT was recorded at the end of the experiment (between 12th and 13th ribs). There were no differences between biotypes in ADG. SB tended to be earlier maturing showing higher BFT (4·39 v. 3·97 mm; P = 0·22). ADG was affected by SR and was higher at lower SR (0·612, 0·529, 0·414 and 0·375 kg/day, for 1·8, 2·3, 2·8 and 3·3 steers per ha, respectively). Individual average daily gain of the MB was more variable across the seasons of the year. None of the variables showed effects of the biotype × SR interaction but differences in ADG between biotypes as a function of the SR were observed as a trend (P = 0·29). Despite the difference in frame score between biotypes it was not possible to associate it with consistent differences in the response variables. Individual productivity (potential) of MB was not reflected in a greater productivity per surface unit. The data from this experiment do not allow the choice of a particular biotype in order to optimize the productivity of the pastoral production systems.

The relative performances of crossbred ewes sired by Finnish Landrace or by non-Finnish rams were measured in trials at 18 farms which yielded a total of about 2600 ewe lambing records. The dams of the crossbred ewes were Hill breeds (40%), Upland (22%), Long-wool x Hill (20%) and other breed types (18%). The non-Finnish sires were Longwool breeds (35 %), Down and Down crosses (33 %), Upland (22%) and other breeds (10%).

Finnish crossbred ewes were lighter at mating than the average of non-Finnish crossbreds by 10% at 7 months, increasing to 24% at 31 and 43 months, with an average difference of 18% for the first 4 years of life. Over the same period of 4 years Finnish crossbreds had a 27 % higher lambing percentage and 21 % higher weaning percentage than non-Finnish crossbreds.

Lambs born to Finnish crossbreds were, over the 4-year period, 16% lighter for singles and 20 % lighter for multiples at birth, and 11 % for single born and 12% for multiple born at 10 weeks, than those born to non-Finnish crossbreds. But the total litter weight of lamb reared to 10 weeks per 50-kg metabolic ewe weight (at mating) was 16% more for Finnish crossbreds than for non-Finnish crossbreds.

This study was carried out to investigate: (1) changes in plasma oestradiol-17β and progesterone concentrations in six pre-pubertal beef heifers, 9 months old, subjected to a superovulatory treatment, consisting of a single dose of PMSG followed 120 h later by a single injection of human chorionic gonadotrophin (HCG); and (2) the ability of this treatment to trigger cyclic gonadal activity. After PMSG administration, oestradiol-17β levels increased from 11·8 (s.d. 10·78) to 92·9 (s.d. 38·15) ng/1 just before HCG injection. The oestradiol-17β increase during the superovulatory treatment and numbe r of ovulations were correlated (r = 0·83; P < 0·05). After HCG injection, progesterone concentrations rose significantly reaching values which in some cases were higher than 60 [μg/1. The progesterone peak on days 9 and 10 post HCG was strongly correlated with the number of corpora lutea (r = 0·95; P < 0·01 and r = 0·92; P < 0·01, respectively). Progesterone determinations were performed for a period of 90 days after HCG administration. No evidence was found for the induction of permanent cyclic ovarian activity since all heifers returned, after the induced luteal phase, to their pre-pubertal condition.

The aim of the present study was to evaluate water absorbing capacity and the drying and cooling rates of fur in farmbred male mink (Mustela vison) following 10 s of simulated diving. Comparisons were made between adult animals with summer and winter fur. The water loss from a swimming pool following diving in summer amounted to 146 (s.e. 14) g and 152 (s.e. 9) g in dry and wet mink, respectively (P > 0·05). During winter the corresponding values averaged 148 (s.e. 9) and 125 (s.e. 7) g, respectively (not significant: P > 0·05). No significant differences between summer and winter were found for dry mink. In wet-coated mink, on the other hand, water loss from the swimming pool was significantly greater (P 0·05) in summer than in winter. Drying of the fur was slow and significantly (P 0·05) affected by season so that half of the 100 g water absorbed by fur evaporated within 20 min during winter (at –2°C) whereas in summer (at +18°C) it took 60 min. A slower drying rate in summer could be benefical as it allows long-term body cooling in warm environment. Cooling constants of winter-coated dry mink (0·01955 (s.e. 0·00183) per min) were not significantly different (P > 0·05) from those of winter-coated wet mink (0·02091 (s.e. 0·00144) per min) indicating that energy costs of wet fur after diving are not critical for the survivial of the mink during winter.

Imported semen from 10 Italian Chianina bulls was used to inseminate beef and dairy cattle throughout England and Southern Scotland. A total of 2134 records of calving performance were subjected to least squares analyses. Dystocia score was significantly affected by county of birth, parity of dam, sire, type of birth, birth weight (P < 0·001), season of birth and sex of calf (P < 0·01). There were no significant effects of breed or size of cow. Serious dystocia was encountered in 3% of calvings, with the incidence for individual sires ranging from 1·7% to 6·7%. Arithmetic mean gestation length was 284·2 days and it was significantly affected by county, sire (P < 0·05), sex of calf (P < 0·01), and type of birth (P < 0·001). Arithmetic mean birth weight of the calves was 41·2 kg and this was significantly affected by all main effects studied except season of birth and type of herd.

It is well established that the genetic merit of the Holstein-Friesian dairy cow for milk production has increased over the past 20 years. Previous studies have examined the effect of feeding system on indices of body tissue reserves of medium genetic merit Holstein-Friesian dairy cows. The aim of the current study was to examine the weight and concentration of body components in high genetic merit Holstein-Friesian dairy cows, managed on four different grassland-based feeding regimes, using direct measures of body composition. Results indicate that there was no significant effect of different grassland-based feeding regimes on the weight or composition of body components of high genetic merit cows. Therefore, high levels of cow performance can be sustained from very different grassland-based systems of milk production without having a detrimental effect on body tissue reserves.

The efficacy of feed additives in enhancing the performance of growing pigs, together with the effect of different protein levels (18 v. 16% from 13 to 18 kg body weight and 16 v. 14% from 18 to 39 kg body-weight), were evaluated in three experiments with 412 pigs. In Experiment 1, from 13 to 39 kg body weight, pigs receiving 55 p.p.m. carbadox (C) produced a greater (P<0·05) average daily live-weight gain than did the non-medicated (NM) pigs and those receiving 275 p.p.m. chlortetracycline-sulphamethazine-penicillin (ASP) with a similar but non-significant (P>0·05) trend n i daily feed intake and gain/feed ratio. The ASP pigs also produced a greater (P<0·05) weight gain than the NM pigs. The higher dietary protein level sequence produced a greater (JP<0·05) weight gain and gain/feed ratio.

In Experiment 2, from 16 to 39 kg body weight, NM control pigs achieved high weight gains, and all the feed additive treatments studied [C+106 p.p.m. of the worming agent pyrantel tartrate (C+P); ASP+8 mg of the worming agent levamisole-HCl per kg body weight fed once on day 7 of the experiment (ASP+L); and 113 p.p.m. tylosin+13 p.p.m. of the worming agent hygromycin B] were similar to the NM controls in daily feed intake, weight gain and gain/feed ratio. However, in Experiment 3, the C+P and ASP+L treatments resulted in a greater (P<0·05) weight gain with a trend toward greater daily feed intake than did the NM control treatment from 17 to 35 kg. The weight gain of the ASP+L pigs was also greater (P<0·05) than that of 165 p.p.m. oxytetracycline and NM pigs from 35 to 57 kg. From 57 to 96 kg all the pigs received the same NM control diet and their weight gains were similar. The gain/feed ratio was similar among the treatments within each of the weight periods in Experiment 3. Pigs slaughtered at the beginning and at various stages during Experiments 2 and 3 were free of internal parasites and migration damage.

The test proofs of 29 Holstein-Friesian sires (12 Canadian and 17 US) with milking daughters in both Canada and the USA were compared. The mean test rating of the Canadian and US sires respectively was 125±74 kg and 265±57 kg higher in Canada than in the United States.

These results were supported by others obtained in milking trials in Denmark and West Germany.

The fat percentage of US Holstein progeny was higher in Canada and Europe than in the USA, suggesting that the low fat percentage of the US Holstein in its country of origin cannot be attributed simply to lack of genetic potential.

It is suggested that the US Holstein be considered as a substitute for the Canadian for the improvement of milking performance in European Friesian populations.

The paper reports the results of experiments to study the effects of different levels of dietary protein on the performance and carcass quality of bacon pigs fed on a high-energy diet: (a) to make similar rates of live-weight gain on all treatments, (b) to make similar rates of live-weight gain but with the level of protein on some treatments being reduced at 130 lb., (c) to allow ad libitum feed intake on all treatments, (d) to obtain two distinct

patterns of growth (fast-fast and slow-fast) on each of two levels of dietary protein.

In the comparison of the two contrasting patterns of growth there was no significant interaction between rate of gain during the period 40–125 lb. live-weight and level of dietary protein. Differences in carcass density and linear measurements were significant and the results suggest that irrespective of growth pattern there was a carcass response to level of protein.

Where diets of different protein content were given ad libitum or to obtain a uniform growth pattern on all treatments, growth rates were generally good, with little difference in feed intake between the treatments within each experiment; variations in level of protein therefore had little effect on growth rate. In some of the comparisons, significant carcass differences were found in terms of density, dissection data and linear measurements; in others only the density differences were significant.

The results suggest that with each of the feeding systems a level of about 17% crude protein, associated with 12% fish meal, is more likely than lower levels to give lean bacon carcasses. In the one experiment in which the protein level was reduced at 130 lb. live-weight there was no marked effect on carcass composition.

Six sheep were used in a Latin-square experiment to investigate the effect of pre-harvest treatment of pasture with glyphosate (Roundup, a translocated herbicide) on the palatability of hay. Four hays were used: control (C), Roundup (R), quinine (Q, 100 mg/kg) and Roundup with quinine (QR). During each week one of the following choices was given to each animal: (a) C/C; (b) C/R; (c) R/R; (d) Q/C; (e) Q/R; and (f) C/QR. There were no significant effects of treatment on the proportion chosen or on total daily intake, mean meal size or rate of eating. It was concluded that neither pre-harvest treatment with Roundup nor post-harvest treatment with quinine affected the palatability of hay.

One hundred and twenty-five boars were given diets containing five levels of methionine: 1·5, 1·7, 1·9, 2·1 and 2·3 g methionine per kg diet, from 21 to 49 days of age. The mean weight gain of the pigs given the diet containing 1·5 g methionine per kg was 71 g/day and this increased linearly to 346 g/day for the pigs given the diets containing 2·3 g methionine per kg.

From 49 to 97 days of age all pigs were given a commercial pig grower diet containing 160 g protein per kg. Live-weight gains over this period increased linearly from 477 to 689 g/day with increasing methionine content of the starter diets. There was no compensatory weight gain.

With the increasing availability of large amounts of data in the livestock domain, we face the challenge to store, combine and analyse these data efficiently. With this study, we explored the use of a data lake for storing and analysing data to improve scalability and interoperability. Data originated from a 2-day animal experiment in which the gait score of approximately 200 turkeys was determined through visual inspection by an expert. Additionally, inertial measurement units (IMUs), a 3D-video camera and a force plate (FP) were installed to explore the effectiveness of these sensors in automating the visual gait scoring. We deployed a data lake using the IMU and FP data of a single day of that animal experiment. This encompasses data from 84 turkeys for which we preprocessed by performing an ‘extract, transform and load’ (ETL-) procedure. To test scalability of the ETL-procedure, we simulated increasing volumes of the available data from this animal experiment and computed the ‘wall time’ (elapsed real time) for converting FP data into comma-separated files and storing these files. With a simulated data set of 30 000 turkeys, the wall time reduced from 1 h to less than 15 min, when 12 cores were used compared to 1 core. This demonstrated the ETL-procedure to be scalable. Subsequently, a machine learning (ML) pipeline was developed to test the potential of a data lake to automatically distinguish between two classses, that is, very bad gait scores v. other scores. In conclusion, we have set up a dedicated customized data lake, loaded data and developed a prediction model via the creation of an ML pipeline. A data lake appears to be a useful tool to face the challenge of storing, combining and analysing increasing volumes of data of varying nature in an effective manner.

N-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) are subject of growing interest as they are of particular relevance for meat quality and human health. However, their content in the muscles of cattle is generally low probably as the complex result of their biosynthesis from dietary n-3 PUFA in the muscle and/or in other tissues/organs and of their subsequent uptake by the muscle. In view of this, this study aimed at understanding whether the changes in the muscle n-3 LCPUFA content, depending on the diet (maize silage v. grass) or the muscle type (Rectus abdominis, RA v.Semitendinosus, ST) in 12 Charolais steers, were related to variations in the gene expression of proteins involved in n-3 LCPUFA biosynthesis or cellular uptake. Tissue fatty acid composition was analysed by gas-liquid chromatography and mRNA abundance of proteins by quantitative real-time PCR. The grass-based diet resulted in a 2.3-fold (P < 0.0002) increase in both RA and ST n-3 LCPUFA content compared with the maize silage-based diet, whereas no difference in the expression of genes involved in n-3 LCPUFA biosynthesis and uptake was observed between diets. ST exhibited a 1.5-fold higher n-3 LCPUFA content than RA (P < 0.003), whereas the gene expression of proteins involved in n-3 LCPUFA biosynthesis and uptake was 1.3- to 18-fold higher in RA than in ST (P < 0.05). In conclusion, diet- or muscle type-dependent changes in the muscle n-3 LCPUFA content of Charolais steers did not seem to be mediated by the gene expression regulation of proteins involved in the biosynthesis or uptake of these fatty acids.

The feed value of whole crop maize silage (WCMS) depends on nutrient composition, ruminal degradability and whole tract digestibility. However, as the ruminal degradation rate is involved in physical regulation of feed intake, ruminal degradability of WCMS may also affect feed intake and milk production of dairy cows. Thus, the aim of this study was to examine relationships between nutrient composition, ruminal degradability, and whole tract digestibility of WCMS and feed intake and milk production of dairy cows. Nine varieties were tested in 3 consecutive years. Nutrient composition analyses included proximate analysis and determination of cell wall constituents. Whole tract digestibility was determined in vivo using wethers and ruminal degradability was examined in situ using four rumen-fistulated steers. Feed intake and milk production were measured using nine cows per variety. Cows were fed a ration consisting of 75.0% WCMS, 8.5% hay and 16.5% soya bean meal (dry matter basis) ad libitum. Variety did not influence nutrient composition, except for the concentration of ADF (ADFom), ADL and utilisable CP (uCP). In contrast, variety had a significant effect (P < 0.05) on ruminal degradability of NDF (aNDFom) and on whole tract digestibility of organic matter (OM) and non-fibre carbohydrates. Dry matter intake (DMI) of WCMS tended to be affected by variety (0.05<P < 0.10) whereas no effect on energy-corrected milk production (ECM) was observed. The year of harvest had an influence on net energy for lactation and uCP concentration, ruminal degradability and whole tract digestibility of nutrients, as well as on DMI and ECM of dairy cows. Whole tract aNDFom digestibility of WCMS was positively correlated with aNDFom concentration (R2 = 0.48) and whole tract OM digestibility (R2 = 0.58). Furthermore, ruminal OM degradability was positively correlated with DMI (R2 = 0.57) and ECM (R2 = 0.49) of dairy cows. The results indicate that ruminal degradability and whole tract digestibility have a greater impact on the feed value of WCMS than nutrient composition and should be focused on to optimise feed intake and milk production of dairy cows. Thus, maize breeders should include ruminal degradability and whole tract digestibility parameters in variety testing programs to increase the informative value of variety descriptions for farmers.

Fermentative catabolism (FAAC) of dietary and endogenous amino acids (AA) in the small intestine contributes to loss of AA available for protein synthesis and body maintenance functions in pigs. A continuous isotope infusion study was performed to determine whole body urea flux, urea recycling and FAAC in the small intestine of ileal-cannulated growing pigs fed a control diet (CON, 18.6% CP; n=6), a high fibre diet with 12% added pectin (HF, 17.7% CP; n=4) or a low-protein diet (LP, 13.4% CP; n=6). 15N-ammonium chloride and 13C-urea were infused intragastrically and intravenously, respectively, for 4 days. Recovery of ammonia at the distal ileum was increased by feeding additional fibre when compared with the CON (P<0.05) but was not affected by dietary protein (0.24, 0.39 and 0.14 mmol nitrogen/kg BW/day for CON, HF and LP, respectively; P<0.05). Lowering protein intake reduced urea flux (25.3, 25.7 and 10.3 mmol nitrogen/kg BW/day; P<0.01), urinary urea excretion (14.4, 15.0 and 6.2 mmol N/kg BW/day; P<0.001) and urea recycling (12.1, 11.3 and 3.23 mmol nitrogen/kg BW/day; P<0.01) compared with CON. There was a rapid reduction in 15N-ammonia enrichment in digesta along the small intestine suggesting rapid absorption of ammonia before the distal ileum and lack of uniformity of enrichment in the digesta ammonia pool. A two-pool model was developed to determine possible value ranges for nitrogen flux in the small intestine assuming rapid absorption of ammonia. Maximum estimated FAAC based on this model was significantly lower when dietary protein content was decreased (32.9, 33.4 and 17.4 mmol nitrogen/kg BW/day; P<0.001). There was no impact of dietary fibre on estimates of small intestine nitrogen flux (P>0.05) compared with CON. The two-pool model developed in the present study allows for estimation of FAAC but still has limitations. Quantifying FAAC in the small intestine of pigs, as well as other non-ruminants and humans, offers a number of challenges but warrants further investigation.

This experiment investigates the effects of maternal nutrient restriction in late gestation on the offsprings’ postnatal metabolism and performance. Forty purebred Shropshire twin lambs born to ewes fed either a high-nutrition diet (H) (according to standard) or a low-nutrition (L) diet (50% during the last 6 weeks of gestation) were studied from birth until 145 days of age. In each feeding group, two different sires were represented, ‘growth’ (G) and ‘meat’ (M), having different breeding indices for the lean : fat ratio. Post partum all ewes were fed the same diet. Lambs born to L-ewes had significantly lower birth weights and pre-weaning growth rates. This was especially pronounced in L-lambs born to the M-ram, which also had markedly lower pre-weaning glucose concentrations than the other three groups of lambs. L-lambs converted milk to live weight with an increased efficiency in week 3 of life. Their glucose concentrations and growth rates were both increased. Plasma glucose concentrations in LM-lambs became similar to those observed in H-lambs post-weaning. However, LM-lambs continued to be lighter than the other groups throughout the experimental period and were unable to compensate for the reduced weight at birth despite having the highest daily fractional growth rates. LG-lambs had the highest plasma glucose concentrations of all four groups of lambs, and they indeed reached body weights comparable to those of the H-lambs by 145 days of age. The increased growth rate post-weaning in L-lambs was not reflected in fat deposition, as L-lambs had lower fat deposition than H-lambs. This may relate to the lower plasma insulin levels found in the L-lambs than in the H-lambs. In conclusion, a 50% reduction of maternal nutrient supply in the last 6 weeks of gestation reduces the birth weight and pre-weaning growth of the offspring due to lower milk intake. Growth rates can be restored when an adequate post-weaning diet is provided, but the prenatal nutrition may programme postnatal metabolism differentially depending on genotype, thus affecting the ability of the ad libitum-fed lamb to achieve a given body weight by a certain age.

Gilt progeny (GP) are born and weaned lighter than sow progeny (SP) and tend to have higher rates of mortality and morbidity. This study quantified the lifetime growth performance differences between GP and SP and, additionally, evaluated whether segregating GP and SP in the grower–finisher period compared to mixing them within common pens reduced this variation. It was hypothesised that GP would be lighter than SP at every stage and segregation would improve growth performance of both GP and SP. All piglets born to 61 gilts (parity 1) and 47 sows (parities 2 to 7; mean 3.5 ± 0.2) were allocated to four treatments at 10 weeks of age: (i) GP housed together (GG), (ii) GP mixed (M) with SP (GM), (iii) SP housed together (SS) and (iv) SP mixed with GP (SM). The GM and SM pigs were housed together in common pens after movement into the grower–finisher facility. Individual live weight of all progeny was recorded at birth, weaning (WWT), 10 weeks of age (10WT) and sale (SWT). Individual hot carcass weight (HCW), fat depth at the head of the last rib (P2) and dressing percentage were measured at slaughter. Gilt progeny were lighter at birth (P = 0.038), weaning (P < 0.001) and through to sale (P = 0.001) than SP. Nursery and grower–finisher performance differences in GP were highly attributable to their lower WWT compared to SP (P < 0.001 when fitted as a covariate). Segregation of GP and SP increased grower–finisher average daily gain (ADG) in SP but decreased ADG and SWT in GP (P < 0.10). Segregated SP had increased average daily feed intake but only in males (P = 0.007); HCW (P < 0.001) and P2 fat depth (P = 0.055) were higher in mixed female GP, but there was no difference (P > 0.10) in female SP, or in males. In conclusion, GP were lighter at every stage than SP and differences after weaning were highly related to the lighter WWT of GP. Under the conditions of this study, overall segregation of GP and SP showed no consistent advantages in growth performance for both groups and differed significantly between males and females.

Ruminants generally succeed in selecting an appropriate diet relative to their nutrient requirements, and macronutrients such as energy and protein are considered as playing a primary role in their food preferences. Diet learning through post-ingestive consequences, evidenced by many studies that involved situations with simple discrimination tasks, may explain this ability to select a suitable diet. However, in more complex feeding environments where animals are faced with many plants varying both in their energy and protein contents, the extent to which such a mechanism operates is still uncertain. Our objective was to investigate a more complex feeding situation than previously tested and to determine whether lambs were able to associate three forages with both energy-based and protein-based post-ingestive consequences of various intensities. The doses of starch and casein used to elicit these post-ingestive consequences were chosen so that the apparent metabolisable energy and crude protein values of hays were within the range found in plants normally encountered under field conditions. We applied five treatments: EmPm, EhPm, EmPh, EhPh, where medium (m) or high (h) levels of nutrients (E = energy and P = protein) were associated with different hays, and E0N0 where no stimuli were associated. Each animal experienced only three treatments: E0N0–EmPm–EhPm or E0N0–EmPh–EhPh, each one being associated with one hay during conditioning, on the basis of one treatment per day over three consecutive days. Animals then had a choice between the hay associated with E0N0 and one of the two others, successively on the two following days. This procedure was repeated through five 1-week-long periods, and applied to two groups of lambs (n = 12) maintained at feeding levels sufficient for maintenance or for a 150 g/day growth rate. This experiment was complemented by a methodological trial that aimed to estimate the doses of starch and casein eliciting preferences and a post-trial that aimed to evaluate the effect of our treatments on sheep ruminal environment. The methodological trial showed that lambs perceived the stimuli and the doses, and the post-trial showed that treatments affected the ruminal environment. In the main experiment, the lambs preferred the rewarded hays but did not discriminate in their choices the variation of post-ingestive consequences due to energy and protein. This suggests that in situations closer to reality where animals experience many foods varying in nutrient contents, the learning process may be impaired. The lambs do not seem to have strictly relied on it for shaping their diet choices.